Nit

• The egg of a louse
• Head lice (often known as "nits" in the United Kingdom, Australia and New Zealand)
• A trivial detail; the object of nitpicking
• Nit (unit), a unit of luminance equivalent to one candela per square metre (1 cd/m²)
• Nits (band), a Dutch musical group
• National Invitation Tournament, a men's college basketball tournament in the United States
• Nagpur Improvement Trust, a local civil government body in Nagpur, India
• Negative income tax, a proposed tax reform
• Neith, an Egyptian goddess
• Nat (information), a logarithmic unit of information or entropy
• Norfolk International Terminals, a port operated by the Virginia Port Authority located in Norfolk, Virginia

Schools

• National Institutes of Technology, 30 engineering schools of national importance located in India
• Naval Institute of Technology, a school in Biliran, Philippines
• Northern Institute of Technology, a school in Germany
• Nekomi Institute of Technology, a fictional university in the anime/manga Oh My Goddess!
• Nagoya Institute of Technology, a college in Nagoya, Japan
• Nippon Institute of Technology, a college in Saitama, Japan
• Nishinippon Institute of Technology, a college in Fukuoka, Japan