NONO

Non-POU domain containing, octamer-binding

PDB rendering based on 2cpj.

Available structures
PDB	2cpj

Identifiers

Symbols

NONO; NMT55; NRB54; P54; P54NRB

External IDs

OMIM: 300084 MGI: 1855692 HomoloGene: 7212 GeneCards: NONO Gene

Gene Ontology
Molecular function	• nucleotide binding • DNA binding • RNA binding • protein binding • identical protein binding
Cellular component	• nucleus • nuclear matrix • paraspeckles
Biological process	• DNA repair • DNA recombination • regulation of transcription, DNA-dependent • mRNA processing • RNA splicing
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr X:
70.5 – 70.52 Mb

Chr X:
98.62 – 98.64 Mb

PubMed search

[1]

[2]

Non-POU domain-containing octamer-binding protein is a protein that in humans is encoded by the NONO gene.^[1]^[2]^[3]

Interactions

NONO has been shown to interact with SFPQ,^[4] SPI1^[5] and Androgen receptor.^[6]

References

^ Dong B, Horowitz DS, Kobayashi R, Krainer AR (Oct 1993). "Purification and cDNA cloning of HeLa cell p54nrb, a nuclear protein with two RNA recognition motifs and extensive homology to human splicing factor PSF and Drosophila NONA/BJ6". Nucleic Acids Res 21 (17): 4085–92. doi:10.1093/nar/21.17.4085. PMC 310009. PMID 8371983. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=310009.
^ Traish AM, Huang YH, Ashba J, Pronovost M, Pavao M, McAneny DB, Moreland RB (Dec 1997). "Loss of expression of a 55 kDa nuclear protein (nmt55) in estrogen receptor-negative human breast cancer". Diagn Mol Pathol 6 (4): 209–21. doi:10.1097/00019606-199708000-00005. PMID 9360842.
^ "Entrez Gene: NONO Non-POU domain containing, octamer-binding". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=4841.
^ Peng, Rui; Dye Billy T, Pérez Ismael, Barnard Daron C, Thompson Amanda B, Patton James G (Oct. 2002). "PSF and p54nrb bind a conserved stem in U5 snRNA". RNA (United States) 8 (10): 1334–47. doi:10.1017/S1355838202022070. ISSN 1355-8382. PMC 1370341. PMID 12403470. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1370341.
^ Hallier, M; Tavitian A, Moreau-Gachelin F (May. 1996). "The transcription factor Spi-1/PU.1 binds RNA and interferes with the RNA-binding protein p54nrb". J. Biol. Chem. (UNITED STATES) 271 (19): 11177–81. doi:10.1074/jbc.271.19.11177. ISSN 0021-9258. PMID 8626664.
^ Ishitani, Ken; Yoshida Tasuku, Kitagawa Hirochika, Ohta Hiroaki, Nozawa Shiro, Kato Shigeaki (Jul. 2003). "p54nrb acts as a transcriptional coactivator for activation function 1 of the human androgen receptor". Biochem. Biophys. Res. Commun. (United States) 306 (3): 660–5. doi:10.1016/S0006-291X(03)01021-0. ISSN 0006-291X. PMID 12810069.

Shav-Tal Y, Zipori D (2002). "PSF and p54(nrb)/NonO--multi-functional nuclear proteins". FEBS Lett. 531 (2): 109–14. doi:10.1016/S0014-5793(02)03447-6. PMID 12417296.
Zhang WW, Zhang LX, Busch RK, et al. (1993). "Purification and characterization of a DNA-binding heterodimer of 52 and 100 kDa from HeLa cells". Biochem. J. 290 ( Pt 1) (Pt 1): 267–72. PMC 1132410. PMID 8439294. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1132410.
Hallier M, Tavitian A, Moreau-Gachelin F (1996). "The transcription factor Spi-1/PU.1 binds RNA and interferes with the RNA-binding protein p54nrb". J. Biol. Chem. 271 (19): 11177–81. doi:10.1074/jbc.271.19.11177. PMID 8626664.
Peters U, Haberhausen G, Kostrzewa M, et al. (1997). "AFX1 and p54nrb: fine mapping, genomic structure, and exclusion as candidate genes of X-linked dystonia parkinsonism". Hum. Genet. 100 (5–6): 569–72. doi:10.1007/s004390050553. PMID 9341872.
Clark J, Lu YJ, Sidhar SK, et al. (1997). "Fusion of splicing factor genes PSF and NonO (p54nrb) to the TFE3 gene in papillary renal cell carcinoma". Oncogene 15 (18): 2233–9. doi:10.1038/sj.onc.1201394. PMID 9393982.
Straub T, Grue P, Uhse A, et al. (1998). "The RNA-splicing factor PSF/p54 controls DNA-topoisomerase I activity by a direct interaction". J. Biol. Chem. 273 (41): 26261–4. doi:10.1074/jbc.273.41.26261. PMID 9756848.
Karhumaa P, Parkkila S, Waheed A, et al. (2000). "Nuclear NonO/p54(nrb) protein is a nonclassical carbonic anhydrase". J. Biol. Chem. 275 (21): 16044–9. doi:10.1074/jbc.275.21.16044. PMID 10821857.
Straub T, Knudsen BR, Boege F (2000). "PSF/p54(nrb) stimulates "jumping" of DNA topoisomerase I between separate DNA helices". Biochemistry 39 (25): 7552–8. doi:10.1021/bi992898e. PMID 10858305.
Mathur M, Tucker PW, Samuels HH (2001). "PSF Is a Novel Corepressor That Mediates Its Effect through Sin3A and the DNA Binding Domain of Nuclear Hormone Receptors". Mol. Cell. Biol. 21 (7): 2298–311. doi:10.1128/MCB.21.7.2298-2311.2001. PMC 86864. PMID 11259580. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=86864.
Zhang Z, Carmichael GG (2001). "The fate of dsRNA in the nucleus: a p54(nrb)-containing complex mediates the nuclear retention of promiscuously A-to-I edited RNAs". Cell 106 (4): 465–75. doi:10.1016/S0092-8674(01)00466-4. PMID 11525732.
Pavao M, Huang YH, Hafer LJ, et al. (2002). "Immunodetection of nmt55/p54nrb isoforms in human breast cancer". BMC Cancer 1: 15. doi:10.1186/1471-2407-1-15. PMC 59838. PMID 11710964. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=59838.
Andersen JS, Lyon CE, Fox AH, et al. (2002). "Directed proteomic analysis of the human nucleolus". Curr. Biol. 12 (1): 1–11. doi:10.1016/S0960-9822(01)00650-9. PMID 11790298.
Sewer MB, Nguyen VQ, Huang CJ, et al. (2002). "Transcriptional activation of human CYP17 in H295R adrenocortical cells depends on complex formation among p54(nrb)/NonO, protein-associated splicing factor, and SF-1, a complex that also participates in repression of transcription". Endocrinology 143 (4): 1280–90. doi:10.1210/en.143.4.1280. PMID 11897684.
Ohta S, Shiomi Y, Sugimoto K, et al. (2002). "A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". J. Biol. Chem. 277 (43): 40362–7. doi:10.1074/jbc.M206194200. PMID 12171929.
Emili A, Shales M, McCracken S, et al. (2002). "Splicing and transcription-associated proteins PSF and p54nrb/nonO bind to the RNA polymerase II CTD". RNA 8 (9): 1102–11. doi:10.1017/S1355838202025037. PMC 1370324. PMID 12358429. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1370324.
Peng R, Dye BT, Pérez I, et al. (2002). "PSF and p54nrb bind a conserved stem in U5 snRNA". RNA 8 (10): 1334–47. doi:10.1017/S1355838202022070. PMC 1370341. PMID 12403470. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1370341.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.

PDB gallery

2cpj: Solution structure of the N-terminal RNA recognition motif of NonO

NONO

Interactions

References

Further reading