NEIL2

Nei endonuclease VIII-like 2 (E. coli)

Identifiers

NEIL2; FLJ31644; MGC2832; MGC4505; NEH2; NEI2

External IDs

OMIM: 608933 MGI: 2686058 HomoloGene: 17032 GeneCards: NEIL2 Gene

4.2.99.18

Gene Ontology
Molecular function	• damaged DNA binding • DNA-(apurinic or apyrimidinic site) lyase activity • zinc ion binding • hydrolase activity, hydrolyzing N-glycosyl compounds • lyase activity • metal ion binding
Cellular component	• nucleus
Biological process	• base-excision repair • nucleotide-excision repair
Sources: Amigo / QuickGO

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 8:
11.63 – 11.64 Mb

Chr 14:
63.8 – 63.81 Mb

PubMed search

[1]

[2]

Endonuclease VIII-like 2 is an enzyme that in humans is encoded by the NEIL2 gene.^[1]^[2]^[3]

NEIL2 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002)[supplied by OMIM]^[3]

References

^ Hazra TK, Kow YW, Hatahet Z, Imhoff B, Boldogh I, Mokkapati SK, Mitra S, Izumi T (Aug 2002). "Identification and characterization of a novel human DNA glycosylase for repair of cytosine-derived lesions". J Biol Chem 277 (34): 30417–20. doi:10.1074/jbc.C200355200. PMID 12097317.
^ Das S, Chattopadhyay R, Bhakat KK, Boldogh I, Kohno K, Prasad R, Wilson SH, Hazra TK (Sep 2007). "Stimulation of NEIL2-mediated oxidized base excision repair via YB-1 interaction during oxidative stress". J Biol Chem 282 (39): 28474–84. doi:10.1074/jbc.M704672200. PMC 2679419. PMID 17686777. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2679419.
^ ^a ^b "Entrez Gene: NEIL2 nei like 2 (E. coli)". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=252969.

Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.". Gene 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination.". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=310948.
Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs.". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=311072.
Takao M, Kanno S, Kobayashi K, et al. (2003). "A back-up glycosylase in Nth1 knock-out mice is a functional Nei (endonuclease VIII) homologue.". J. Biol. Chem. 277 (44): 42205–13. doi:10.1074/jbc.M206884200. PMID 12200441.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Bandaru V, Sunkara S, Wallace SS, Bond JP (2003). "A novel human DNA glycosylase that removes oxidative DNA damage and is homologous to Escherichia coli endonuclease VIII.". DNA Repair (Amst.) 1 (7): 517–29. doi:10.1016/S1568-7864(02)00036-8. PMID 12509226.
Dou H, Mitra S, Hazra TK (2004). "Repair of oxidized bases in DNA bubble structures by human DNA glycosylases NEIL1 and NEIL2.". J. Biol. Chem. 278 (50): 49679–84. doi:10.1074/jbc.M308658200. PMID 14522990.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Katafuchi A, Nakano T, Masaoka A, et al. (2004). "Differential specificity of human and Escherichia coli endonuclease III and VIII homologues for oxidative base lesions.". J. Biol. Chem. 279 (14): 14464–71. doi:10.1074/jbc.M400393200. PMID 14734554.
Bhakat KK, Hazra TK, Mitra S (2004). "Acetylation of the human DNA glycosylase NEIL2 and inhibition of its activity.". Nucleic Acids Res. 32 (10): 3033–9. doi:10.1093/nar/gkh632. PMC 434438. PMID 15175427. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=434438.
Das A, Rajagopalan L, Mathura VS, et al. (2004). "Identification of a zinc finger domain in the human NEIL2 (Nei-like-2) protein.". J. Biol. Chem. 279 (45): 47132–8. doi:10.1074/jbc.M406224200. PMID 15339932.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to biology: a functional genomics pipeline.". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528930.
Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006.". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1347501.
Das A, Wiederhold L, Leppard JB, et al. (2007). "NEIL2-initiated, APE-independent repair of oxidized bases in DNA: Evidence for a repair complex in human cells.". DNA Repair (Amst.) 5 (12): 1439–48. doi:10.1016/j.dnarep.2006.07.003. PMC 2805168. PMID 16982218. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2805168.
Broderick P, Bagratuni T, Vijayakrishnan J, et al. (2006). "Evaluation of NTHL1, NEIL1, NEIL2, MPG, TDG, UNG and SMUG1 genes in familial colorectal cancer predisposition.". BMC Cancer 6: 243. doi:10.1186/1471-2407-6-243. PMC 1624846. PMID 17029639. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1624846.
Katafuchi A, Matsubara M, Terato H, et al. (2007). "Damage specificity of human DNA glycosylases for oxidative pyrimidine lesions.". Nucleic Acids Symp Ser (Oxf) 48 (48): 175–6. doi:10.1093/nass/48.1.175. PMID 17150535.

NEIL2

References

Further reading