MLLT4

Myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4

PDB rendering based on 1t2m.
Identifiers
Symbols MLLT4; AF6; FLJ34371; RP3-431P23.3
External IDs OMIM: 159559 MGI: 1314653 HomoloGene: 21202 GeneCards: MLLT4 Gene
RNA expression pattern
More reference expression data
Orthologs
Species Human Mouse
Entrez 4301 17356
Ensembl ENSG00000130396 ENSMUSG00000068036
UniProt P55196 Q3TLN8
RefSeq (mRNA) NM_001040000.2 NM_010806.1
RefSeq (protein) NP_001035089.1 NP_034936.1
Location (UCSC) Chr 6:
168.23 – 168.37 Mb		 Chr 17:
13.9 – 14.04 Mb
PubMed search [1] [2]

Afadin is a protein that in humans is encoded by the MLLT4 gene.[1]

AF6 is a Ras (see HRAS; MIM 190020) target that regulates cell–cell adhesions downstream of Ras activation. It is fused with MLL (MIM 159555) in leukemias caused by t(6;11) translocations (Taya et al., 1998).[supplied by OMIM][2]

Interactions

MLLT4 has been shown to interact with F11 receptor,[3] RAP1A,[4] LMO2,[5] EPHB3,[6] RAP1GAP,[7] PVRL1,[8] PVRL3,[9][10] USP9X,[11][12] SORBS1,[13] BCR gene,[14] Tight junction protein 1,[15] Profilin 1,[4] HRAS[4][15] and SSX2IP.[16]

References

  1. ^ Prasad R, Gu Y, Alder H, Nakamura T, Canaani O, Saito H, Huebner K, Gale RP, Nowell PC, Kuriyama K, et al. (Jan 1994). "Cloning of the ALL-1 fusion partner, the AF-6 gene, involved in acute myeloid leukemias with the t(6;11) chromosome translocation". Cancer Res 53 (23): 5624–8. PMID 8242616. 
  2. ^ "Entrez Gene: MLLT4 myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=4301. 
  3. ^ Ebnet, K; Schulz C U, Meyer Zu Brickwedde M K, Pendl G G, Vestweber D (Sep. 2000). "Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1". J. Biol. Chem. (UNITED STATES) 275 (36): 27979–88. doi:10.1074/jbc.M002363200. ISSN 0021-9258. PMID 10856295. 
  4. ^ a b c Boettner, B; Govek E E, Cross J, Van Aelst L (Aug. 2000). "The junctional multidomain protein AF-6 is a binding partner of the Rap1A GTPase and associates with the actin cytoskeletal regulator profilin". Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 97 (16): 9064–9. doi:10.1073/pnas.97.16.9064. ISSN 0027-8424. PMC 16822. PMID 10922060. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=16822. 
  5. ^ Bégay-Müller, Valérie; Ansieau Stéphane, Leutz Achim (Jun. 2002). "The LIM domain protein Lmo2 binds to AF6, a translocation partner of the MLL oncogene". FEBS Lett. (Netherlands) 521 (1–3): 36–8. doi:10.1016/S0014-5793(02)02814-4. ISSN 0014-5793. PMID 12067721. 
  6. ^ Hock, B; Böhme B, Karn T, Yamamoto T, Kaibuchi K, Holtrich U, Holland S, Pawson T, Rübsamen-Waigmann H, Strebhardt K (Aug. 1998). "PDZ-domain-mediated interaction of the Eph-related receptor tyrosine kinase EphB3 and the ras-binding protein AF6 depends on the kinase activity of the receptor". Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 95 (17): 9779–84. doi:10.1073/pnas.95.17.9779. ISSN 0027-8424. PMC 21413. PMID 9707552. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=21413. 
  7. ^ Su, Li; Hattori Masakazu, Moriyama Masaki, Murata Norihito, Harazaki Masashi, Kaibuchi Kozo, Minato Nagahiro (Apr. 2003). "AF-6 controls integrin-mediated cell adhesion by regulating Rap1 activation through the specific recruitment of Rap1GTP and SPA-1". J. Biol. Chem. (United States) 278 (17): 15232–8. doi:10.1074/jbc.M211888200. ISSN 0021-9258. PMID 12590145. 
  8. ^ Takahashi, K; Nakanishi H, Miyahara M, Mandai K, Satoh K, Satoh A, Nishioka H, Aoki J, Nomoto A, Mizoguchi A, Takai Y (May. 1999). "Nectin/PRR: an immunoglobulin-like cell adhesion molecule recruited to cadherin-based adherens junctions through interaction with Afadin, a PDZ domain-containing protein". J. Cell Biol. (UNITED STATES) 145 (3): 539–49. doi:10.1083/jcb.145.3.539. ISSN 0021-9525. PMC 2185068. PMID 10225955. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2185068. 
  9. ^ Satoh-Horikawa, K; Nakanishi H, Takahashi K, Miyahara M, Nishimura M, Tachibana K, Mizoguchi A, Takai Y (Apr. 2000). "Nectin-3, a new member of immunoglobulin-like cell adhesion molecules that shows homophilic and heterophilic cell-cell adhesion activities". J. Biol. Chem. (UNITED STATES) 275 (14): 10291–9. doi:10.1074/jbc.275.14.10291. ISSN 0021-9258. PMID 10744716. 
  10. ^ Reymond, N; Borg J P, Lecocq E, Adelaide J, Campadelli-Fiume G, Dubreuil P, Lopez M (Sep. 2000). "Human nectin3/PRR3: a novel member of the PVR/PRR/nectin family that interacts with afadin". Gene (NETHERLANDS) 255 (2): 347–55. doi:10.1016/S0378-1119(00)00316-4. ISSN 0378-1119. PMID 11024295. 
  11. ^ Taya, S; Yamamoto T, Kanai-Azuma M, Wood S A, Kaibuchi K (Dec. 1999). "The deubiquitinating enzyme Fam interacts with and stabilizes beta-catenin". Genes Cells (ENGLAND) 4 (12): 757–67. doi:10.1046/j.1365-2443.1999.00297.x. ISSN 1356-9597. PMID 10620020. 
  12. ^ Taya, S; Yamamoto T, Kano K, Kawano Y, Iwamatsu A, Tsuchiya T, Tanaka K, Kanai-Azuma M, Wood S A, Mattick J S, Kaibuchi K (Aug. 1998). "The Ras target AF-6 is a substrate of the fam deubiquitinating enzyme". J. Cell Biol. (UNITED STATES) 142 (4): 1053–62. doi:10.1083/jcb.142.4.1053. ISSN 0021-9525. PMC 2132865. PMID 9722616. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2132865. 
  13. ^ Mandai, K; Nakanishi H, Satoh A, Takahashi K, Satoh K, Nishioka H, Mizoguchi A, Takai Y (Mar. 1999). "Ponsin/SH3P12: an l-afadin- and vinculin-binding protein localized at cell-cell and cell-matrix adherens junctions". J. Cell Biol. (UNITED STATES) 144 (5): 1001–17. doi:10.1083/jcb.144.5.1001. ISSN 0021-9525. PMC 2148189. PMID 10085297. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2148189. 
  14. ^ Radziwill, G; Erdmann R A, Margelisch U, Moelling K (Jul. 2003). "The Bcr kinase downregulates Ras signaling by phosphorylating AF-6 and binding to its PDZ domain". Mol. Cell. Biol. (United States) 23 (13): 4663–72. doi:10.1128/MCB.23.13.4663-4672.2003. ISSN 0270-7306. PMC 164848. PMID 12808105. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=164848. 
  15. ^ a b Yamamoto, T; Harada N, Kawano Y, Taya S, Kaibuchi K (May. 1999). "In vivo interaction of AF-6 with activated Ras and ZO-1". Biochem. Biophys. Res. Commun. (UNITED STATES) 259 (1): 103–7. doi:10.1006/bbrc.1999.0731. ISSN 0006-291X. PMID 10334923. 
  16. ^ Asada, Masanori; Irie Kenji, Morimoto Koji, Yamada Akio, Ikeda Wataru, Takeuchi Masakazu, Takai Yoshimi (Feb. 2003). "ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions". J. Biol. Chem. (United States) 278 (6): 4103–11. doi:10.1074/jbc.M209832200. ISSN 0021-9258. PMID 12446711. 

Further reading