MCM7

Minichromosome maintenance complex component 7

Identifiers

MCM7; CDC47; MCM2; P1.1-MCM3; P1CDC47; P85MCM; PNAS146

External IDs

OMIM: 600592 MGI: 1298398 HomoloGene: 4323 GeneCards: MCM7 Gene

3.6.4.12

Gene Ontology
Molecular function	• nucleotide binding • DNA binding • single-stranded DNA binding • ATP-dependent DNA helicase activity • protein binding • ATP binding • hydrolase activity
Cellular component	• chromatin • nucleus • nucleoplasm • nucleoplasm • MCM complex • MCM complex
Biological process	• cell cycle checkpoint • G1/S transition of mitotic cell cycle • S phase of mitotic cell cycle • M/G1 transition of mitotic cell cycle • mitotic cell cycle • DNA replication • DNA unwinding involved in replication • DNA-dependent DNA replication initiation • DNA strand elongation involved in DNA replication • response to DNA damage stimulus • cell proliferation • regulation of phosphorylation
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 7:
99.69 – 99.7 Mb

Chr 5:
138.61 – 138.61 Mb

PubMed search

[1]

[2]

DNA replication licensing factor MCM7 is a protein that in humans is encoded by the MCM7 gene.^[1]

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.^[2]

Interactions

MCM7 has been shown to interact with Replication protein A1,^[3] ORC3L,^[3] Retinoblastoma protein,^[4] MCM5,^[3]^[5]^[6] MCM6,^[7]^[3]^[5]^[8] ORC1L,^[3] ORC2L,^[3] ORC5L,^[3] MCM4,^[7]^[5]^[9]^[8] MCM3,^[3]^[10]^[5]^[11] CDC6,^[3]^[10] MNAT1,^[12] DBF4,^[3] MCM2,^[7]^[3]^[13]^[5]^[8] UBE3A,^[14] CDC45-related protein^[3]^[15] and Cell division cycle 7-related protein kinase.^[3]

References

^ Nakatsuru S, Sudo K, Nakamura Y (Mar 1995). "Isolation and mapping of a human gene (MCM2) encoding a product homologous to yeast proteins involved in DNA replication". Cytogenet Cell Genet 68 (3-4): 226–30. doi:10.1159/000133918. PMID 7842741.
^ "Entrez Gene: MCM7 MCM7 minichromosome maintenance deficient 7 (S. cerevisiae)". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=4176.
^ ^a ^b ^c ^d ^e ^f ^g ^h ⁱ ^j ^k ^l ^m Kneissl, Margot; Pütter Vera, Szalay Aladar A, Grummt Friedrich (Mar. 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol. (England) 327 (1): 111–28. doi:10.1016/S0022-2836(03)00079-2. ISSN 0022-2836. PMID 12614612.
^ Sterner, J M; Dew-Knight S, Musahl C, Kornbluth S, Horowitz J M (May. 1998). "Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7". Mol. Cell. Biol. (UNITED STATES) 18 (5): 2748–57. ISSN 0270-7306. PMC 110654. PMID 9566894. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=110654.
^ ^a ^b ^c ^d ^e Fujita, M; Kiyono T, Hayashi Y, Ishibashi M (Apr. 1997). "In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP". J. Biol. Chem. (UNITED STATES) 272 (16): 10928–35. doi:10.1074/jbc.272.16.10928. ISSN 0021-9258. PMID 9099751.
^ DaFonseca, C J; Shu F, Zhang J J (Mar. 2001). "Identification of two residues in MCM5 critical for the assembly of MCM complexes and Stat1-mediated transcription activation in response to IFN-gamma". Proc. Natl. Acad. Sci. U.S.A. (United States) 98 (6): 3034–9. doi:10.1073/pnas.061487598. ISSN 0027-8424. PMC 30602. PMID 11248027. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=30602.
^ ^a ^b ^c Yabuta, Norikazu; Kajimura Naoko, Mayanagi Kouta, Sato Michio, Gotow Takahito, Uchiyama Yasuo, Ishimi Yukio, Nojima Hiroshi (May. 2003). "Mammalian Mcm2/4/6/7 complex forms a toroidal structure". Genes Cells (England) 8 (5): 413–21. doi:10.1046/j.1365-2443.2003.00645.x. ISSN 1356-9597. PMID 12694531.
^ ^a ^b ^c You, Zhiying; Ishimi Yukio, Masai Hisao, Hanaoka Fumio (Nov. 2002). "Roles of Mcm7 and Mcm4 subunits in the DNA helicase activity of the mouse Mcm4/6/7 complex". J. Biol. Chem. (United States) 277 (45): 42471–9. doi:10.1074/jbc.M205769200. ISSN 0021-9258. PMID 12207017.
^ Ishimi, Y; Ichinose S, Omori A, Sato K, Kimura H (Sep. 1996). "Binding of human minichromosome maintenance proteins with histone H3". J. Biol. Chem. (UNITED STATES) 271 (39): 24115–22. doi:10.1074/jbc.271.39.24115. ISSN 0021-9258. PMID 8798650.
^ ^a ^b Fujita, M; Yamada C, Goto H, Yokoyama N, Kuzushima K, Inagaki M, Tsurumi T (Sep. 1999). "Cell cycle regulation of human CDC6 protein. Intracellular localization, interaction with the human mcm complex, and CDC2 kinase-mediated hyperphosphorylation". J. Biol. Chem. (UNITED STATES) 274 (36): 25927–32. doi:10.1074/jbc.274.36.25927. ISSN 0021-9258. PMID 10464337.
^ Fujita, Masatoshi; Ishimi Yukio, Nakamura Hiromu, Kiyono Tohru, Tsurumi Tatsuya (Mar. 2002). "Nuclear organization of DNA replication initiation proteins in mammalian cells". J. Biol. Chem. (United States) 277 (12): 10354–61. doi:10.1074/jbc.M111398200. ISSN 0021-9258. PMID 11779870.
^ Wang, Y; Xu F, Hall F L (Oct. 2000). "The MAT1 cyclin-dependent kinase-activating kinase (CAK) assembly/targeting factor interacts physically with the MCM7 DNA licensing factor". FEBS Lett. (NETHERLANDS) 484 (1): 17–21. doi:10.1016/S0014-5793(00)02117-7. ISSN 0014-5793. PMID 11056214.
^ You, Z; Komamura Y, Ishimi Y (Dec. 1999). "Biochemical analysis of the intrinsic Mcm4-Mcm6-mcm7 DNA helicase activity". Mol. Cell. Biol. (UNITED STATES) 19 (12): 8003–15. ISSN 0270-7306. PMC 84885. PMID 10567526. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=84885.
^ Kühne, C; Banks L (Dec. 1998). "E3-ubiquitin ligase/E6-AP links multicopy maintenance protein 7 to the ubiquitination pathway by a novel motif, the L2G box". J. Biol. Chem. (UNITED STATES) 273 (51): 34302–9. doi:10.1074/jbc.273.51.34302. ISSN 0021-9258. PMID 9852095.
^ Kukimoto, I; Igaki H, Kanda T (Nov. 1999). "Human CDC45 protein binds to minichromosome maintenance 7 protein and the p70 subunit of DNA polymerase alpha". Eur. J. Biochem. (GERMANY) 265 (3): 936–43. doi:10.1046/j.1432-1327.1999.00791.x. ISSN 0014-2956. PMID 10518787.

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Musahl C, Schulte D, Burkhart R, Knippers R (1995). "A human homologue of the yeast replication protein Cdc21. Interactions with other Mcm proteins.". Eur. J. Biochem. 230 (3): 1096–101. doi:10.1111/j.1432-1033.1995.tb20660.x. PMID 7601140.
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Fujita M, Kiyono T, Hayashi Y, Ishibashi M (1996). "hCDC47, a human member of the MCM family. Dissociation of the nucleus-bound form during S phase.". J. Biol. Chem. 271 (8): 4349–54. doi:10.1074/jbc.271.8.4349. PMID 8626784.
Schulte D, Richter A, Burkhart R, et al. (1996). "Properties of the human nuclear protein p85Mcm. Expression, nuclear localization and interaction with other Mcm proteins.". Eur. J. Biochem. 235 (1-2): 144–51. doi:10.1111/j.1432-1033.1996.00144.x. PMID 8631321.
Kiyono T, Fujita M, Hayashi Y, Ishibashi M (1996). "Cloning of a cDNA encoding a human homologue of CDC47, a member of the MCM family.". Biochim. Biophys. Acta 1307 (1): 31–4. PMID 8652665.
Ishimi Y, Ichinose S, Omori A, et al. (1996). "Binding of human minichromosome maintenance proteins with histone H3.". J. Biol. Chem. 271 (39): 24115–22. doi:10.1074/jbc.271.39.24115. PMID 8798650.
Fujita M, Kiyono T, Hayashi Y, Ishibashi M (1997). "In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP.". J. Biol. Chem. 272 (16): 10928–35. doi:10.1074/jbc.272.16.10928. PMID 9099751.
Yu W, Andersson B, Worley KC, et al. (1997). "Large-scale concatenation cDNA sequencing.". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139146.
Hiraiwa A, Fujita M, Nagasaka T, et al. (1997). "Immunolocalization of hCDC47 protein in normal and neoplastic human tissues and its relation to growth.". Int. J. Cancer 74 (2): 180–4. doi:10.1002/(SICI)1097-0215(19970422)74:2<180::AID-IJC7>3.0.CO;2-V. PMID 9133452.
Ishimi Y (1997). "A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex.". J. Biol. Chem. 272 (39): 24508–13. doi:10.1074/jbc.272.39.24508. PMID 9305914.
Sterner JM, Dew-Knight S, Musahl C, et al. (1998). "Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7.". Mol. Cell. Biol. 18 (5): 2748–57. PMC 110654. PMID 9566894. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=110654.
Suzuki S, Adachi A, Hiraiwa A, et al. (1998). "Cloning and characterization of human MCM7 promoter.". Gene 216 (1): 85–91. doi:10.1016/S0378-1119(98)00323-0. PMID 9714754.
Kühne C, Banks L (1999). "E3-ubiquitin ligase/E6-AP links multicopy maintenance protein 7 to the ubiquitination pathway by a novel motif, the L2G box.". J. Biol. Chem. 273 (51): 34302–9. doi:10.1074/jbc.273.51.34302. PMID 9852095.
Fujita M, Yamada C, Goto H, et al. (1999). "Cell cycle regulation of human CDC6 protein. Intracellular localization, interaction with the human mcm complex, and CDC2 kinase-mediated hyperphosphorylation.". J. Biol. Chem. 274 (36): 25927–32. doi:10.1074/jbc.274.36.25927. PMID 10464337.
Freeman A, Morris LS, Mills AD, et al. (1999). "Minichromosome maintenance proteins as biological markers of dysplasia and malignancy.". Clin. Cancer Res. 5 (8): 2121–32. PMID 10473096.
Kukimoto I, Igaki H, Kanda T (1999). "Human CDC45 protein binds to minichromosome maintenance 7 protein and the p70 subunit of DNA polymerase alpha.". Eur. J. Biochem. 265 (3): 936–43. doi:10.1046/j.1432-1327.1999.00791.x. PMID 10518787.

MCM7

See also

Interactions

References