MCF2L

MCF.2 cell line derived transforming sequence-like

PDB rendering based on 1kz7.

Available structures
PDB	1kz7, 1kzg, 1lb1, 1rj2

Identifiers

Symbols

MCF2L; ARHGEF14; DBS; FLJ12122; KIAA0362; OST

External IDs

OMIM: 609499 MGI: 103263 HomoloGene: 11804 GeneCards: MCF2L Gene

Gene Ontology
Molecular function	• guanyl-nucleotide exchange factor activity • Rho guanyl-nucleotide exchange factor activity
Cellular component	• cytoplasm • cytosol • cytosol • plasma membrane
Biological process	• apoptosis • small GTPase mediated signal transduction • induction of apoptosis by extracellular signals • regulation of Rho protein signal transduction • nerve growth factor receptor signaling pathway • regulation of small GTPase mediated signal transduction
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 13:
113.55 – 113.75 Mb

Chr 8:
12.87 – 13.02 Mb

PubMed search

[1]

[2]

Guanine nucleotide exchange factor DBS is a protein that in humans is encoded by the MCF2L gene.^[1]^[2]

References

^ Nagase T, Ishikawa K, Nakajima D, Ohira M, Seki N, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Sep 1997). "Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res 4 (2): 141–50. doi:10.1093/dnares/4.2.141. PMID 9205841.
^ "Entrez Gene: MCF2L MCF.2 cell line derived transforming sequence-like". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=23263.

Horii Y, Beeler JF, Sakaguchi K, et al. (1994). "A novel oncogene, ost, encodes a guanine nucleotide exchange factor that potentially links Rho and Rac signaling pathways.". EMBO J. 13 (20): 4776–86. PMC 395416. PMID 7957046. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=395416.
Rossman KL, Worthylake DK, Snyder JT, et al. (2002). "A crystallographic view of interactions between Dbs and Cdc42: PH domain-assisted guanine nucleotide exchange.". EMBO J. 21 (6): 1315–26. doi:10.1093/emboj/21.6.1315. PMC 125919. PMID 11889037. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=125919.
Snyder JT, Worthylake DK, Rossman KL, et al. (2002). "Structural basis for the selective activation of Rho GTPases by Dbl exchange factors.". Nat. Struct. Biol. 9 (6): 468–75. doi:10.1038/nsb796. PMID 12006984.
Yamauchi J, Hirasawa A, Miyamoto Y, et al. (2002). "Role of Dbl's big sister in the anti-mitogenic pathway from alpha1B-adrenergic receptor to c-Jun N-terminal kinase.". Biochem. Biophys. Res. Commun. 296 (1): 85–92. doi:10.1016/S0006-291X(02)00839-2. PMID 12147231.
Wennerberg K, Ellerbroek SM, Liu RY, et al. (2003). "RhoG signals in parallel with Rac1 and Cdc42.". J. Biol. Chem. 277 (49): 47810–7. doi:10.1074/jbc.M203816200. PMID 12376551.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Cheng L, Mahon GM, Kostenko EV, Whitehead IP (2004). "Pleckstrin homology domain-mediated activation of the rho-specific guanine nucleotide exchange factor Dbs by Rac1.". J. Biol. Chem. 279 (13): 12786–93. doi:10.1074/jbc.M313099200. PMID 14701795.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Ueda S, Kataoka T, Satoh T (2005). "Role of the Sec14-like domain of Dbl family exchange factors in the regulation of Rho family GTPases in different subcellular sites.". Cell. Signal. 16 (8): 899–906. doi:10.1016/j.cellsig.2004.01.007. PMID 15157669.
Kostenko EV, Mahon GM, Cheng L, Whitehead IP (2005). "The Sec14 homology domain regulates the cellular distribution and transforming activity of the Rho-specific guanine nucleotide exchange factor Dbs.". J. Biol. Chem. 280 (4): 2807–17. doi:10.1074/jbc.M411139200. PMID 15531584.
Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes.". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1356129.
Ieguchi K, Ueda S, Kataoka T, Satoh T (2007). "Role of the guanine nucleotide exchange factor Ost in negative regulation of receptor endocytosis by the small GTPase Rac1.". J. Biol. Chem. 282 (32): 23296–305. doi:10.1074/jbc.M700950200. PMID 17562712.

PDB gallery

1kz7: Crystal Structure of the DH/PH Fragment of Murine Dbs in Complex with the Placental Isoform of Human Cdc42

1kzg: DbsCdc42(Y889F)

1lb1: Crystal Structure of the Dbl and Pleckstrin homology domains of Dbs in complex with RhoA

1rj2: Crystal structure of the DH/PH fragment of Dbs without bound GTPase

MCF2L

References

Further reading