MCF2

MCF.2 cell line derived transforming sequence

Identifiers

MCF2; ARHGEF21; DBL

External IDs

OMIM: 311030 MGI: 96932 HomoloGene: 3917 GeneCards: MCF2 Gene

Gene Ontology
Molecular function	• guanyl-nucleotide exchange factor activity • Rho guanyl-nucleotide exchange factor activity • protein binding
Cellular component	• membrane fraction • cytoplasm • cytosol • cytosol • cytoskeleton • membrane
Biological process	• apoptosis • small GTPase mediated signal transduction • induction of apoptosis by extracellular signals • regulation of Rho protein signal transduction • nerve growth factor receptor signaling pathway • regulation of small GTPase mediated signal transduction
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

n/a

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr X:
138.66 – 138.79 Mb

Chr X:
57.31 – 57.43 Mb

PubMed search

[1]

[2]

Proto-oncogene DBL is a protein that in humans is encoded by the MCF2 gene.^[1]^[2]^[3]

MCF2 is a member of a large family of GDP-GTP exchange factors that modulate the activity of small GTPases of the Rho family. Five-prime recombinations result in the loss of N-terminal codons, producing MCF2 variants with oncogenic potential.[supplied by OMIM]^[3]

References

^ Noguchi T, Galland F, Batoz M, Mattei MG, Birnbaum D (Apr 1994). "Activation of a mcf.2 oncogene by deletion of amino-terminal coding sequences". Oncogene 3 (6): 709–15. PMID 2577874.
^ Galland F, Stefanova M, Lafage M, Birnbaum D (Jul 1992). "Localization of the 5' end of the MCF2 oncogene to human chromosome 15q15----q23". Cytogenet Cell Genet 60 (2): 114–6. doi:10.1159/000133316. PMID 1611909.
^ ^a ^b "Entrez Gene: MCF2 MCF.2 cell line derived transforming sequence". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=4168.

Ron D, Zannini M, Lewis M et al. (1991). "A region of proto-dbl essential for its transforming activity shows sequence similarity to a yeast cell cycle gene, CDC24, and the human breakpoint cluster gene, bcr". New Biol. 3 (4): 372–9. PMID 2065022.
Tronick SR, McBride OW, Popescu NC, Eva A (1990). "Chromosomal localization of DBL oncogene sequences". Genomics 5 (3): 546–53. doi:10.1016/0888-7543(89)90022-0. PMID 2613238.
Ron D, Tronick SR, Aaronson SA, Eva A (1989). "Molecular cloning and characterization of the human dbl proto-oncogene: evidence that its overexpression is sufficient to transform NIH/3T3 cells". EMBO J. 7 (8): 2465–73. PMC 457116. PMID 3056717. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=457116.
Eva A, Vecchio G, Rao CD et al. (1988). "The predicted DBL oncogene product defines a distinct class of transforming proteins". Proc. Natl. Acad. Sci. U.S.A. 85 (7): 2061–5. doi:10.1073/pnas.85.7.2061. PMC 279928. PMID 3281159. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=279928.
Srivastava SK, Wheelock RH, Aaronson SA, Eva A (1987). "Identification of the protein encoded by the human diffuse B-cell lymphoma (dbl) oncogene". Proc. Natl. Acad. Sci. U.S.A. 83 (23): 8868–72. doi:10.1073/pnas.83.23.8868. PMC 387034. PMID 3491366. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=387034.
Hart MJ, Eva A, Zangrilli D et al. (1994). "Cellular transformation and guanine nucleotide exchange activity are catalyzed by a common domain on the dbl oncogene product". J. Biol. Chem. 269 (1): 62–5. PMID 8276860.
Nishida K, Kaziro Y, Satoh T (1999). "Association of the proto-oncogene product dbl with G protein betagamma subunits". FEBS Lett. 459 (2): 186–90. doi:10.1016/S0014-5793(99)01244-2. PMID 10518015.
Kato J, Kaziro Y, Satoh T (2000). "Activation of the guanine nucleotide exchange factor Dbl following ACK1-dependent tyrosine phosphorylation". Biochem. Biophys. Res. Commun. 268 (1): 141–7. doi:10.1006/bbrc.2000.2106. PMID 10652228.
Zhu K, Debreceni B, Li R, Zheng Y (2000). "Identification of Rho GTPase-dependent sites in the Dbl homology domain of oncogenic Dbl that are required for transformation". J. Biol. Chem. 275 (34): 25993–6001. doi:10.1074/jbc.M003780200. PMID 10854437.
Palmieri G, de Franciscis V, Casamassimi A et al. (2000). "Human dbl proto-oncogene in 85 kb of xq26, and determination of the transcription initiation site". Gene 253 (1): 107–15. doi:10.1016/S0378-1119(00)00212-2. PMID 10925207.
Jin S, Exton JH (2000). "Activation of RhoA by association of Galpha(13) with Dbl". Biochem. Biophys. Res. Commun. 277 (3): 718–21. doi:10.1006/bbrc.2000.3744. PMID 11062019.
Zhu K, Debreceni B, Bi F, Zheng Y (2001). "Oligomerization of DH domain is essential for Dbl-induced transformation". Mol. Cell. Biol. 21 (2): 425–37. doi:10.1128/MCB.21.2.425-437.2001. PMC 86589. PMID 11134331. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=86589.
Vanni C, Mancini P, Gao Y et al. (2002). "Regulation of proto-Dbl by intracellular membrane targeting and protein stability". J. Biol. Chem. 277 (22): 19745–53. doi:10.1074/jbc.M111025200. PMID 11907027.
Wennerberg K, Ellerbroek SM, Liu RY et al. (2003). "RhoG signals in parallel with Rac1 and Cdc42". J. Biol. Chem. 277 (49): 47810–7. doi:10.1074/jbc.M203816200. PMID 12376551.
Komai K, Okayama R, Kitagawa M et al. (2003). "Alternative splicing variants of the human DBL (MCF-2) proto-oncogene". Biochem. Biophys. Res. Commun. 299 (3): 455–8. doi:10.1016/S0006-291X(02)02645-1. PMID 12445822.
Komai K, Mukae-Sakairi N, Kitagawa M, Shiozawa S (2003). "Characterization of novel splicing variants of the mouse MCF-2 (DBL) proto-oncogene". Biochem. Biophys. Res. Commun. 309 (4): 906–9. doi:10.1016/j.bbrc.2003.08.088. PMID 13679059.
Ross MT, Grafham DV, Coffey AJ et al. (2005). "The DNA sequence of the human X chromosome". Nature 434 (7031): 325–37. doi:10.1038/nature03440. PMC 2665286. PMID 15772651. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2665286.
Kimura K, Wakamatsu A, Suzuki Y et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1356129.

MCF2

References

Further reading