MBD3

Methyl-CpG binding domain protein 3
Identifiers
Symbols MBD3;
External IDs OMIM603573 MGI1333812 HomoloGene2917 GeneCards: MBD3 Gene
RNA expression pattern
More reference expression data
Orthologs
Species Human Mouse
Entrez 53615 17192
Ensembl ENSG00000071655 ENSMUSG00000035478
UniProt O95983 Q9Z2D8
RefSeq (mRNA) NM_003926 NM_013595.2
RefSeq (protein) NP_003917 NP_038623.1
Location (UCSC) Chr 19:
1.58 – 1.59 Mb
Chr 10:
79.86 – 79.86 Mb
PubMed search [1] [2]

Methyl-CpG-binding domain protein 3 is a protein that in humans is encoded by the MBD3 gene.[1][2][3]

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex.[3]

Interactions

MBD3 has been shown to interact with Aurora A kinase,[4] HDAC1,[4][5][6] Methyl-CpG-binding domain protein 2,[5][7] MTA2[4][5][6] and GATAD2B.[8][9]

References

  1. ^ Hendrich B, Bird A (Nov 1998). "Identification and characterization of a family of mammalian methyl-CpG binding proteins". Mol Cell Biol 18 (11): 6538–47. PMC 109239. PMID 9774669. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=109239. 
  2. ^ Hendrich B, Abbott C, McQueen H, Chambers D, Cross S, Bird A (Sep 1999). "Genomic structure and chromosomal mapping of the murine and human Mbd1, Mbd2, Mbd3, and Mbd4 genes". Mamm Genome 10 (9): 906–12. doi:10.1007/s003359901112. PMID 10441743. 
  3. ^ a b "Entrez Gene: MBD3 methyl-CpG binding domain protein 3". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=53615. 
  4. ^ a b c Sakai, Hirotaka; Urano Takeshi, Ookata Kayoko, Kim Mi-Hyun, Hirai Yugo, Saito Motoki, Nojima Yoshihisa, Ishikawa Fuyuki (Dec. 2002). "MBD3 and HDAC1, two components of the NuRD complex, are localized at Aurora-A-positive centrosomes in M phase". J. Biol. Chem. (United States) 277 (50): 48714–23. doi:10.1074/jbc.M208461200. ISSN 0021-9258. PMID 12354758. 
  5. ^ a b c Zhang, Y; Ng H H, Erdjument-Bromage H, Tempst P, Bird A, Reinberg D (Aug. 1999). "Analysis of the NuRD subunits reveals a histone deacetylase core complex and a connection with DNA methylation". Genes Dev. (UNITED STATES) 13 (15): 1924–35. doi:10.1101/gad.13.15.1924. ISSN 0890-9369. PMC 316920. PMID 10444591. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=316920. 
  6. ^ a b Saito, Motoki; Ishikawa Fuyuki (Sep. 2002). "The mCpG-binding domain of human MBD3 does not bind to mCpG but interacts with NuRD/Mi2 components HDAC1 and MTA2". J. Biol. Chem. (United States) 277 (38): 35434–9. doi:10.1074/jbc.M203455200. ISSN 0021-9258. PMID 12124384. 
  7. ^ Jiang, Chun-Ling; Jin Seung-Gi, Pfeifer Gerd P (Dec. 2004). "MBD3L1 is a transcriptional repressor that interacts with methyl-CpG-binding protein 2 (MBD2) and components of the NuRD complex". J. Biol. Chem. (United States) 279 (50): 52456–64. doi:10.1074/jbc.M409149200. ISSN 0021-9258. PMID 15456747. 
  8. ^ Brackertz, Marc; Boeke Joern, Zhang Ru, Renkawitz Rainer (Oct. 2002). "Two highly related p66 proteins comprise a new family of potent transcriptional repressors interacting with MBD2 and MBD3". J. Biol. Chem. (United States) 277 (43): 40958–66. doi:10.1074/jbc.M207467200. ISSN 0021-9258. PMID 12183469. 
  9. ^ Feng, Qin; Cao Ru, Xia Li, Erdjument-Bromage Hediye, Tempst Paul, Zhang Yi (Jan. 2002). "Identification and functional characterization of the p66/p68 components of the MeCP1 complex". Mol. Cell. Biol. (United States) 22 (2): 536–46. doi:10.1128/MCB.22.2.536-546.2002. ISSN 0270-7306. PMC 139742. PMID 11756549. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139742. 

Further reading