MAP2K1IP1

Late endosomal/lysosomal adaptor, MAPK and MTOR activator 3

PDB rendering based on 1sko.

Available structures
PDB	1SKO, 2ZL1, 3CPT

Identifiers

Symbols

LAMTOR3; MAP2K1IP1; MAPBP; MAPKSP1; MP1; Ragulator3

External IDs

OMIM: 603296 HomoloGene: 10539 GeneCards: LAMTOR3 Gene

Gene Ontology
Molecular function	• protein binding
Cellular component	• endosome • membrane • Ragulator complex
Biological process	• positive regulation of TOR signaling cascade • cellular protein localization • cellular response to amino acid stimulus
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

n/a

n/a

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 4:
100.8 – 100.82 Mb

n/a

PubMed search

[1]

[2]

Mitogen-activated protein kinase scaffold protein 1 is a scaffold protein that in humans is encoded by the MAPKSP1 gene.^[1]^[2]^[3]

The protein encoded by this gene was identified as an interacting protein that binds specifically to MAP kinase kinase MAP2K1/MEK1 and to MAP kinase MAPK2/ERK1. This protein enhances the activation of MAPK2, and thus is thought to function as an adaptor to enhance the efficiency of the MAP kinase cascade.^[3]

Interactions

MAP2K1IP1 has been shown to interact with MAP2K1.^[4]^[1]

References

^ ^a ^b Schaeffer HJ, Catling AD, Eblen ST, Collier LS, Krauss A, Weber MJ (Oct 1998). "MP1: a MEK binding partner that enhances enzymatic activation of the MAP kinase cascade". Science 281 (5383): 1668–71. doi:10.1126/science.281.5383.1668. PMID 9733512.
^ Lunin VV, Munger C, Wagner J, Ye Z, Cygler M, Sacher M (May 2004). "The structure of the MAPK scaffold, MP1, bound to its partner, p14. A complex with a critical role in endosomal map kinase signaling". J Biol Chem 279 (22): 23422–30. doi:10.1074/jbc.M401648200. PMID 15016825.
^ ^a ^b "Entrez Gene: MAP2K1IP1 mitogen-activated protein kinase kinase 1 interacting protein 1". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=8649.
^ Wunderlich, W; Fialka I, Teis D, Alpi A, Pfeifer A, Parton R G, Lottspeich F, Huber L A (Feb. 2001). "A Novel 14-Kilodalton Protein Interacts with the Mitogen-Activated Protein Kinase Scaffold Mp1 on a Late Endosomal/Lysosomal Compartment". J. Cell Biol. (United States) 152 (4): 765–76. doi:10.1083/jcb.152.4.765. ISSN 0021-9525. PMC 2195784. PMID 11266467. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2195784.

Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Okabe S, Tauchi T, Morita H et al. (1999). "Thrombopoietin induces an SH2-containing protein, CIS1, which binds to Mpl: involvement of the ubiquitin proteosome pathway". Exp. Hematol. 27 (10): 1542–7. doi:10.1016/S0301-472X(99)00094-6. PMID 10517496.
Wunderlich W, Fialka I, Teis D et al. (2001). "A Novel 14-Kilodalton Protein Interacts with the Mitogen-Activated Protein Kinase Scaffold Mp1 on a Late Endosomal/Lysosomal Compartment". J. Cell Biol. 152 (4): 765–76. doi:10.1083/jcb.152.4.765. PMC 2195784. PMID 11266467. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2195784.
Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Teis D, Wunderlich W, Huber LA (2003). "Localization of the MP1-MAPK scaffold complex to endosomes is mediated by p14 and required for signal transduction". Dev. Cell 3 (6): 803–14. doi:10.1016/S1534-5807(02)00364-7. PMID 12479806.
Channavajhala PL, Wu L, Cuozzo JW et al. (2004). "Identification of a novel human kinase supporter of Ras (hKSR-2) that functions as a negative regulator of Cot (Tpl2) signaling". J. Biol. Chem. 278 (47): 47089–97. doi:10.1074/jbc.M306002200. PMID 12975377.
Paces-Fessy M, Boucher D, Petit E et al. (2004). "The negative regulator of Gli, Suppressor of fused (Sufu), interacts with SAP18, Galectin3 and other nuclear proteins". Biochem. J. 378 (Pt 2): 353–62. doi:10.1042/BJ20030786. PMC 1223961. PMID 14611647. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1223961.
Ota T, Suzuki Y, Nishikawa T et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Vomastek T, Schaeffer HJ, Tarcsafalvi A et al. (2004). "Modular construction of a signaling scaffold: MORG1 interacts with components of the ERK cascade and links ERK signaling to specific agonists". Proc. Natl. Acad. Sci. U.S.A. 101 (18): 6981–6. doi:10.1073/pnas.0305894101. PMC 406452. PMID 15118098. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=406452.
Kurzbauer R, Teis D, de Araujo ME et al. (2004). "Crystal structure of the p14/MP1 scaffolding complex: How a twin couple attaches mitogen-activated protein kinase signaling to late endosomes". Proc. Natl. Acad. Sci. U.S.A. 101 (30): 10984–9. doi:10.1073/pnas.0403435101. PMC 503730. PMID 15263099. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=503730.
Gerhard DS, Wagner L, Feingold EA et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Sharma C, Vomastek T, Tarcsafalvi A et al. (2005). "MEK partner 1 (MP1): regulation of oligomerization in MAP kinase signaling". J. Cell. Biochem. 94 (4): 708–19. doi:10.1002/jcb.20344. PMID 15547943.
Pullikuth A, McKinnon E, Schaeffer HJ, Catling AD (2005). "The MEK1 Scaffolding Protein MP1 Regulates Cell Spreading by Integrating PAK1 and Rho Signals". Mol. Cell. Biol. 25 (12): 5119–33. doi:10.1128/MCB.25.12.5119-5133.2005. PMC 1140582. PMID 15923628. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1140582.
Villegas SN, Njaine B, Linden R, Carri NG (2007). "Glial-derived neurotrophic factor (GDNF) prevents ethanol (EtOH) induced B92 glial cell death by both PI3K/AKT and MEK/ERK signaling pathways". Brain Res. Bull. 71 (1–3): 116–26. doi:10.1016/j.brainresbull.2006.08.014. PMID 17113937.
Teis D, Taub N, Kurzbauer R et al. (2007). "p14–MP1-MEK1 signaling regulates endosomal traffic and cellular proliferation during tissue homeostasis". J. Cell Biol. 175 (6): 861–8. doi:10.1083/jcb.200607025. PMC 2064696. PMID 17178906. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2064696.
Brahma A, Dalby KN (2007). "Regulation of Protein Phosphorylation within the MKK1-ERK2 Complex by MP1 and the MP1•P14 Heterodimer". Arch. Biochem. Biophys. 460 (1): 85–91. doi:10.1016/j.abb.2006.11.031. PMC 2853909. PMID 17254543. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2853909.
Vomastek T, Iwanicki MP, Schaeffer HJ et al. (2007). "RACK1 Targets the Extracellular Signal-Regulated Kinase/Mitogen-Activated Protein Kinase Pathway To Link Integrin Engagement with Focal Adhesion Disassembly and Cell Motility". Mol. Cell. Biol. 27 (23): 8296–305. doi:10.1128/MCB.00598-07. PMC 2169169. PMID 17908799. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2169169.

PDB gallery

1sko: MP1-p14 Complex

1vet: Crystal Structure of p14/MP1 at 1.9 A resolution

1veu: Crystal structure of the p14/MP1 complex at 2.15 A resolution

MAP2K1IP1

Interactions

References

Further reading