| Lagovirus | |
|---|---|
| Virus classification | |
| Group: | Group IV ((+)ssRNA) |
| Family: | Caliciviridae |
| Genus: | Vesivirus |
| Synonyms | |
Contents |
Virions consist of a capsid. Virus capsid is not enveloped, round with icosahedral symmetry. The isometric capsid has a diameter of 35-39 nm. Capsids appear round to hexagonal in outline. The capsid surface structure reveals a regular pattern with distinctive features. The capsomer arrangement is clearly visible. Capsid with 32 cup-shaped depressions.
The molecular mass (Mr) of virions is 15 x 106. Virions have a buoyant density in Caesium chloride (CsCl) of 1.33-1.36 g/cm3. The density gradient of virions in Potassium Tartrate-Glycerol is 1.29 g/cm3. The sedimentation coefficient is 170-187 svedberg (s20,w); of the other(s) are peak 160-170 svedberg (s20,w) (believed to consist of defective interfering particles). Under in vitro conditions virions are inactivated in acid environment of pH 4.5-7; stable in alkaline environment of pH 7-10.5. Virions are not stable at raised temperature in presence of high concentration of Mg++. Virions are sensitive to treatment with trypsin (in some strains, not sensitive to treatment with mild detergents, or ether, or chloroform. The infectivity is enhanced after treatment with trypsin (in some strains).
The genome is not segmented and contains a single molecule of linear positive-sense, ssRNA. Minor species of non-genomic nucleic acid are some times also found in virions. The encapsidated nucleic acid is mainly of genomic origin, but virions may also contain subgenomic RNA. The complete genome is 7450 nucleotides long. The genome has a guanine + cytosine content of 49.3-50.1 %. The 5' end of the genome has a usually genome-linked protein (VPg), or methylated nucleotide cap (in the case of Hepatitis E virus). The 3' terminus has a poly (A) tract. Each virion contains a full length copy, or defective interfering copies.
The viral genome encodes viral structural protein. Virions consist of 1 structural protein(s) (major species located in the capsid.
Viral structural protein: Capsid protein has a molar mass of 59000-71000 Da; is the coat protein. Capsid protein has a molecular mass of minor 'soluble' 28-30 kDa.
Lipids are not reported.
By itself, genomic nucleic acid is infectious.
Transcription: Subgenomic mRNA is present in infected cells.
Cross-reactivity is found. Cross-reactivity between species of the same serotype, but not with species of another serotype and some species of the same serotype, but not with all. Although the degree of antigenic specificity varies with the degree of relatedness, the antigenicity is distinct from serogroups of the same genus. Most species in the genus are related antigenically. They are sharing some epitopes in the structural proteins.