ITSN2

Intersectin 2

PDB rendering based on 1j3t.

Available structures
PDB	1J3T, 1UDL, 1UE9, 1UFF, 1UHF, 3GF9, 3JZY

Identifiers

Symbols

ITSN2; KIAA1256; PRO2015; SH3D1B; SH3P18; SWA; SWAP

External IDs

OMIM: 604464 MGI: 1338049 HomoloGene: 22627 GeneCards: ITSN2 Gene

Gene Ontology
Molecular function	• SH3/SH2 adaptor activity • Rho guanyl-nucleotide exchange factor activity • calcium ion binding • protein binding
Cellular component	• intracellular • cytoplasm
Biological process	• endocytosis • regulation of Rho protein signal transduction
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 2:
24.43 – 24.58 Mb

Chr 12:
4.6 – 4.72 Mb

PubMed search

[1]

[2]

Intersectin-2 is a protein that in humans is encoded by the ITSN2 gene.^[1]^[2]^[3]

This gene encodes a cytoplasmic protein which contains SH3 domains. This protein is a member of a family of proteins involved in clathrin-mediated endocytosis. Intersectin 2 is thought to regulate the formation of clathrin-coated vesicles and also may function in the induction of T cell antigen receptor (TCR) endocytosis. Alternatively spliced transcript variants have been found for this gene that encode three distinct isoforms. Additional variants have been found but their full length nature has not been determined.^[3]

Interactions

ITSN2 has been shown to interact with Wiskott-Aldrich syndrome protein.^[2]

References

^ Pucharcos C, Estivill X, de la Luna S (Aug 2000). "Intersectin 2, a new multimodular protein involved in clathrin-mediated endocytosis". FEBS Lett 478 (1–2): 43–51. doi:10.1016/S0014-5793(00)01793-2. PMID 10922467.
^ ^a ^b McGavin MK, Badour K, Hardy LA, Kubiseski TJ, Zhang J, Siminovitch KA (Dec 2001). "The intersectin 2 adaptor links Wiskott Aldrich Syndrome protein (WASp)-mediated actin polymerization to T cell antigen receptor endocytosis". J Exp Med 194 (12): 1777–87. doi:10.1084/jem.194.12.1777. PMC 2193569. PMID 11748279. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2193569.
^ ^a ^b "Entrez Gene: ITSN2 intersectin 2". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=50618.

Andersson B, Wentland MA, Ricafrente JY, et al. (1996). "A "double adaptor" method for improved shotgun library construction.". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
Yu W, Andersson B, Worley KC, et al. (1997). "Large-scale concatenation cDNA sequencing.". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139146.
Sparks AB, Hoffman NG, McConnell SJ, et al. (1998). "Cloning of ligand targets: systematic isolation of SH3 domain-containing proteins.". Nat. Biotechnol. 14 (6): 741–4. doi:10.1038/nbt0696-741. PMID 9630982.
Nagase T, Ishikawa K, Kikuno R, et al. (2000). "Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.". DNA Res. 6 (5): 337–45. doi:10.1093/dnares/6.5.337. PMID 10574462.
Pucharcos C, Casas C, Nadal M, et al. (2001). "The human intersectin genes and their spliced variants are differentially expressed.". Biochim. Biophys. Acta 1521 (1–3): 1–11. PMID 11690630.
Nakayama M, Kikuno R, Ohara O (2003). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs.". Genome Res. 12 (11): 1773–84. doi:10.1101/gr.406902. PMC 187542. PMID 12421765. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=187542.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Chini CC, Chen J (2003). "Human claspin is required for replication checkpoint control.". J. Biol. Chem. 278 (32): 30057–62. doi:10.1074/jbc.M301136200. PMID 12766152.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Brill LM, Salomon AR, Ficarro SB, et al. (2004). "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry.". Anal. Chem. 76 (10): 2763–72. doi:10.1021/ac035352d. PMID 15144186.
Lehner B, Sanderson CM (2004). "A protein interaction framework for human mRNA degradation.". Genome Res. 14 (7): 1315–23. doi:10.1101/gr.2122004. PMC 442147. PMID 15231747. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=442147.
Rush J, Moritz A, Lee KA, et al. (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.". Nat. Biotechnol. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455.
Zhang Y, Wolf-Yadlin A, Ross PL, et al. (2005). "Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules.". Mol. Cell Proteomics 4 (9): 1240–50. doi:10.1074/mcp.M500089-MCP200. PMID 15951569.
Nishimura T, Yamaguchi T, Tokunaga A, et al. (2006). "Role of numb in dendritic spine development with a Cdc42 GEF intersectin and EphB2.". Mol. Biol. Cell 17 (3): 1273–85. doi:10.1091/mbc.E05-07-0700. PMC 1382316. PMID 16394100. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1382316.
Seifert M, Ampofo C, Mehraein Y, et al. (2007). "Expression analysis of human intersectin 2 gene (ITSN2) minor splice variants showing differential expression in normal human brain.". Oncol. Rep. 17 (5): 1207–11. PMID 17390067.
Lim CS, Seet BT, Ingham RJ, et al. (2007). "The K15 protein of Kaposi's sarcoma-associated herpesvirus recruits the endocytic regulator intersectin 2 through a selective SH3 domain interaction.". Biochemistry 46 (35): 9874–85. doi:10.1021/bi700357s. PMID 17696407.

PDB gallery

1j3t: Solution structure of the second SH3 domain of human intersectin 2 (KIAA1256)

1udl: The solution structure of the fifth SH3 domain of intersectin 2 (KIAA1256)

1ue9: Solution structure of the fourth SH3 domain of human intersectin 2 (KIAA1256)

1uff: Solution structure of the first SH3 domain of human intersectin2 (KIAA1256)

1uhf: Solution Structure of the third SH3 domain of human intersectin 2(KIAA1256)

ITSN2

Interactions

References

Further reading