IRIS (Biosensor)

Interferometric Reflectance Imaging Sensor (IRIS) is a system that was formerly known as the Spectral Reflectance Imaging Biosensor (SRIB).[1] It can be used a biosensing platform that is capable of high-throughput multiplexing of protein-protein, protein-DNA, and DNA-DNA interactions without the use of any fluorescent labels. The sensing surface is prepared by robotic spotting of biological probes that are immobilized on functionalized Si/SiO2 substrates. IRIS is capable of quantifying bimolecular mass accumulated on the surface[2]

Contents

Measurement

To perform a measurement, the sample is illuminated with multiple different wavelengths from either a tunable laser or different color LEDs; typically speaking, a relatively narrow bandwidth optical source. The reflection intensity is imaged using a CCD or CMOS camera. By using interferometric techniques, nanometer changes can be detected.

Applications

Applications for IRIS include microarray format immunoassays, single nucleotide polymorphism (SNP)[3] detection, pathogen detection and bio-defense monitoring, kinetic analysis of biomolecular interactions, and general biomolecular interaction studies for research applications.

References

  1. ^ E. Ozkumur et al. (2008). "Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applications". Proceedings of the National Academy of Science 105: 7988–7992. doi:10.1073/pnas.0711421105. PMID 18523019. 
  2. ^ E. Ozkumur et al. (2009). "Quantification of DNA and protein adsorption by optical phase shift". Biosensors and Bioelectronics 25: 167–172. doi:10.1016/j.bios.2009.06.033. PMID 19628383. 
  3. ^ E. Ozkumur et al. (2010). "Label-free microarray imaging for direct detection of DNA hybridization and single-nucleotide mismatches". Biosensors and Bioelectronics 25: 1789–1795. doi:10.1016/j.bios.2009.12.032. PMID 20097056. 

Further reading