HIP2

Ubiquitin-conjugating enzyme E2K

PDB rendering based on 1yla.

Available structures
PDB	1YLA, 2O25, 3E46, 3F92, 3K9O, 3K9P

Identifiers

Symbols

UBE2K; DKFZp564C1216; DKFZp686J24237; E2-25K; HIP2; HYPG; LIG; UBC1

External IDs

OMIM: 602846 MGI: 1858216 HomoloGene: 3903 GeneCards: UBE2K Gene

EC number

6.3.2.19

Gene Ontology
Molecular function	• nucleotide binding • ubiquitin-protein ligase activity • protein binding • ATP binding • ligase activity • acid-amino acid ligase activity • ubiquitin protein ligase binding • ubiquitin-ubiquitin ligase activity
Cellular component	• cytoplasm
Biological process	• ubiquitin-dependent protein catabolic process • protein ubiquitination • protein K48-linked ubiquitination
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 4:
39.7 – 39.78 Mb

Chr 5:
65.93 – 65.99 Mb

PubMed search

[1]

[2]

Ubiquitin-conjugating enzyme E2 K is a protein that in humans is encoded by the UBE2K gene.^[1]^[2]^[3]

The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. It binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. This protein has been implicated in the degradation of huntingtin and suppression of apoptosis.^[3]

Interactions

HIP2 has been shown to interact with Huntingtin^[1]^[4] and RNF2.^[5]

References

^ ^a ^b Kalchman MA, Graham RK, Xia G, Koide HB, Hodgson JG, Graham KC, Goldberg YP, Gietz RD, Pickart CM, Hayden MR (Sep 1996). "Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme". J Biol Chem 271 (32): 19385–94. doi:10.1074/jbc.271.32.19385. PMID 8702625.
^ Christensen DE, Brzovic PS, Klevit RE (Oct 2007). "E2-BRCA1 RING interactions dictate synthesis of mono- or specific polyubiquitin chain linkages". Nat Struct Mol Biol 14 (10): 941–8. doi:10.1038/nsmb1295. PMID 17873885.
^ ^a ^b "Entrez Gene: HIP2 huntingtin interacting protein 2". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=3093.
^ Faber, P W; Barnes G T, Srinidhi J, Chen J, Gusella J F, MacDonald M E (Sep. 1998). "Huntingtin interacts with a family of WW domain proteins". Hum. Mol. Genet. (ENGLAND) 7 (9): 1463–74. doi:10.1093/hmg/7.9.1463. ISSN 0964-6906. PMID 9700202.
^ Lee, S J; Choi J Y, Sung Y M, Park H, Rhim H, Kang S (Aug. 2001). "E3 ligase activity of RING finger proteins that interact with Hip-2, a human ubiquitin-conjugating enzyme". FEBS Lett. (Netherlands) 503 (1): 61–4. doi:10.1016/S0014-5793(01)02689-8. ISSN 0014-5793. PMID 11513855.

Petersén A, Mani K, Brundin P (1999). "Recent advances on the pathogenesis of Huntington's disease.". Exp. Neurol. 157 (1): 1–18. doi:10.1006/exnr.1998.7006. PMID 10222105.
Coux O, Goldberg AL (1998). "Enzymes catalyzing ubiquitination and proteolytic processing of the p105 precursor of nuclear factor kappaB1.". J. Biol. Chem. 273 (15): 8820–8. doi:10.1074/jbc.273.15.8820. PMID 9535861.
Whitby FG, Xia G, Pickart CM, Hill CP (1999). "Crystal structure of the human ubiquitin-like protein NEDD8 and interactions with ubiquitin pathway enzymes.". J. Biol. Chem. 273 (52): 34983–91. doi:10.1074/jbc.273.52.34983. PMID 9857030.
Kikuchi J, Furukawa Y, Kubo N, et al. (2000). "Induction of ubiquitin-conjugating enzyme by aggregated low density lipoprotein in human macrophages and its implications for atherosclerosis.". Arterioscler. Thromb. Vasc. Biol. 20 (1): 128–34. doi:10.1161/01.ATV.20.1.128. PMID 10634809.
Furukawa Y, Kubo N, Kikuchi J, et al. (2000). "Regulation of macrophage-specific gene expression by degenerated lipoproteins.". Electrophoresis 21 (2): 338–46. doi:10.1002/(SICI)1522-2683(20000101)21:2<338::AID-ELPS338>3.0.CO;2-9. PMID 10675012.
Lee SJ, Choi JY, Sung YM, et al. (2001). "E3 ligase activity of RING finger proteins that interact with Hip-2, a human ubiquitin-conjugating enzyme.". FEBS Lett. 503 (1): 61–4. doi:10.1016/S0014-5793(01)02689-8. PMID 11513855.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Gevaert K, Goethals M, Martens L, et al. (2004). "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.". Nat. Biotechnol. 21 (5): 566–9. doi:10.1038/nbt810. PMID 12665801.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Goehler H, Lalowski M, Stelzl U, et al. (2004). "A protein interaction network links GIT1, an enhancer of huntingtin aggregation, to Huntington's disease.". Mol. Cell 15 (6): 853–65. doi:10.1016/j.molcel.2004.09.016. PMID 15383276.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes.". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1356129.
Flierman D, Coleman CS, Pickart CM, et al. (2006). "E2-25K mediates US11-triggered retro-translocation of MHC class I heavy chains in a permeabilized cell system.". Proc. Natl. Acad. Sci. U.S.A. 103 (31): 11589–94. doi:10.1073/pnas.0605215103. PMC 1520313. PMID 16868077. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1520313.
de Pril R, Fischer DF, Roos RA, van Leeuwen FW (2007). "Ubiquitin-conjugating enzyme E2-25K increases aggregate formation and cell death in polyglutamine diseases.". Mol. Cell. Neurosci. 34 (1): 10–9. doi:10.1016/j.mcn.2006.09.006. PMID 17092742.
Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry.". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1847948.

PDB gallery

1yla: Ubiquitin-conjugating enzyme E2-25 kDa (Huntington interacting protein 2)

2bep: CRYSTAL STRUCTURE OF UBIQUITIN CONJUGATING ENZYME E2-25K

2bf8: CRYSTAL STRUCTURE OF SUMO MODIFIED UBIQUITIN CONJUGATING ENZYME E2-25K

2o25: Ubiquitin-Conjugating Enzyme E2-25 kDa Complexed With SUMO-1-Conjugating Enzyme UBC9

HIP2

Interactions

References

Further reading