Higher-energy collisional dissociation

Higher-energy collisional dissociation (HCD, formerly higher-energy C-trap dissociation) is a fragmentation technique, often used for peptide modification analysis. Immonium ions generated via HCD pinpoint modifications such as phosphotyrosine with very high confidence. An added octopole collision cell facilitates de novo sequencing.[1]

References

  1. ^ Olsen JV, Macek B, Lange O, Makarov A, Horning S, Mann M (September 2007). "Higher-energy C-trap dissociation for peptide modification analysis". Nat. Methods 4 (9): 709–12. doi:10.1038/nmeth1060. PMID 17721543. http://www.nature.com/nmeth/journal/v4/n9/full/nmeth1060.html. 
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