GlmY RNA

Glm Y RNA activator of glmS mRNA
Predicted secondary structure and sequence conservation of GlmY_tke1
Identifiers
Symbol GlmY_tke1
Alt. Symbols tke1
Rfam RF00128
Other data
RNA type Gene; sRNA
Domain(s) Bacteria
SO 0000655

The GlmY RNA (formally known as tke1) family consists of a number of bacterial RNA genes of around 167 bases in length. The GlmY RNA gene is present in Escherichia coli, Shigella flexneri, Yersinia pestis and Salmonella species, where it is found between the yfhK and purL genes. It was originally predicted in a bioinformatic screen for novel ncRNAs in E. coli.[1]

The GlmY sRNA has been shown to activate the synthesis of GlmS.[2] It achieves this by influencing the action of another sRNA called GlmZ in a hierarchical fashion.[3][4][5]

Further studies have shown that GlmY mutants are sensitive to cell envelope stress.[6]

References

  1. ^ Rivas, E; Klein RJ, Jones TA, Eddy SR (2001). "Computational identification of noncoding RNAs in E. coli by comparative genomics". Curr Biol 11 (17): 1369–1373. doi:10.1016/S0960-9822(01)00401-8. PMID 11553332. 
  2. ^ Urban JH, Papenfort K, Thomsen J, Schmitz RA, Vogel J (2007). "A conserved small RNA promotes discoordinate expression of the glmUS operon mRNA to activate GlmS synthesis.". J Mol Biol 373 (3): 521–8. doi:10.1016/j.jmb.2007.07.035. PMID 17854828. 
  3. ^ Reichenbach B, Maes A, Kalamorz F, Hajnsdorf E, Görke B (2008). "The small RNA GlmY acts upstream of the sRNA GlmZ in the activation of glmS expression and is subject to regulation by polyadenylation in Escherichia coli.". Nucleic Acids Res 36 (8): 2570–80. doi:10.1093/nar/gkn091. PMC 2377431. PMID 18334534. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2377431. 
  4. ^ Urban JH, Vogel J (2008). "Two seemingly homologous noncoding RNAs act hierarchically to activate glmS mRNA translation.". PLoS Biol 6 (3): e64. doi:10.1371/journal.pbio.0060064. PMC 2267818. PMID 18351803. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2267818. 
  5. ^ Görke B, Vogel J (2008). "Noncoding RNA control of the making and breaking of sugars.". Genes Dev 22 (21): 2914–25. doi:10.1101/gad.1717808. PMID 18981470. 
  6. ^ Hobbs EC, Astarita JL, Storz G (2010). "Small RNAs and small proteins involved in resistance to cell envelope stress and acid shock in Escherichia coli: analysis of a bar-coded mutant collection.". J Bacteriol 192 (1): 59–67. doi:10.1128/JB.00873-09. PMC 2798238. PMID 19734312. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2798238. 

External links