GORASP1

Golgi reassembly stacking protein 1, 65kDa

Identifiers

GORASP1; FLJ23443; GOLPH5; GRASP65; MGC118894; MGC118897; P65

External IDs

OMIM: 606867 MGI: 1921748 HomoloGene: 49916 GeneCards: GORASP1 Gene

Gene Ontology
Molecular function	• protein binding
Cellular component	• Golgi apparatus • cytosol • membrane
Biological process	• M phase of mitotic cell cycle • mitotic prophase • mitotic cell cycle • Golgi organization • protein transport
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 3:
39.14 – 39.15 Mb

Chr 9:
119.83 – 119.85 Mb

PubMed search

[1]

[2]

Golgi reassembly-stacking protein 1 is a protein that in humans is encoded by the GORASP1 gene.^[1]

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined.^[1]

Interactions

GORASP1 has been shown to interact with TGF alpha,^[2] TMED2^[2] and GOLGA2.^[2]^[3]^[4]^[5]

References

^ ^a ^b "Entrez Gene: GORASP1 golgi reassembly stacking protein 1, 65kDa". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=64689.
^ ^a ^b ^c Barr, F A; Preisinger C, Kopajtich R, Körner R (Dec. 2001). "Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus". J. Cell Biol. (United States) 155 (6): 885–91. doi:10.1083/jcb.200108102. ISSN 0021-9525. PMC 2150891. PMID 11739402. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2150891.
^ Marra, P; Maffucci T, Daniele T, Tullio G D, Ikehara Y, Chan E K, Luini A, Beznoussenko G, Mironov A, De Matteis M A (Dec. 2001). "The GM130 and GRASP65 Golgi proteins cycle through and define a subdomain of the intermediate compartment". Nat. Cell Biol. (England) 3 (12): 1101–13. doi:10.1038/ncb1201-1101. ISSN 1465-7392. PMID 11781572.
^ Short, B; Preisinger C, Körner R, Kopajtich R, Byron O, Barr F A (Dec. 2001). "A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic". J. Cell Biol. (United States) 155 (6): 877–83. doi:10.1083/jcb.200108079. ISSN 0021-9525. PMC 2150909. PMID 11739401. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2150909.
^ Shorter, J; Watson R, Giannakou M E, Clarke M, Warren G, Barr F A (Sep. 1999). "GRASP55, a second mammalian GRASP protein involved in the stacking of Golgi cisternae in a cell-free system". EMBO J. (ENGLAND) 18 (18): 4949–60. doi:10.1093/emboj/18.18.4949. ISSN 0261-4189. PMC 1171566. PMID 10487747. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1171566.

Barr FA, Puype M, Vandekerckhove J, Warren G (1997). "GRASP65, a protein involved in the stacking of Golgi cisternae.". Cell 91 (2): 253–62. doi:10.1016/S0092-8674(00)80407-9. PMID 9346242.
Barr FA, Nakamura N, Warren G (1998). "Mapping the interaction between GRASP65 and GM130, components of a protein complex involved in the stacking of Golgi cisternae.". EMBO J. 17 (12): 3258–68. doi:10.1093/emboj/17.12.3258. PMC 1170664. PMID 9628863. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1170664.
Lin CY, Madsen ML, Yarm FR, et al. (2001). "Peripheral Golgi protein GRASP65 is a target of mitotic polo-like kinase (Plk) and Cdc2.". Proc. Natl. Acad. Sci. U.S.A. 97 (23): 12589–94. doi:10.1073/pnas.220423497. PMC 18808. PMID 11050165. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=18808.
Moyer BD, Allan BB, Balch WE (2001). "Rab1 interaction with a GM130 effector complex regulates COPII vesicle cis--Golgi tethering.". Traffic 2 (4): 268–76. doi:10.1034/j.1600-0854.2001.1o007.x. PMID 11285137.
Sütterlin C, Lin CY, Feng Y, et al. (2001). "Polo-like kinase is required for the fragmentation of pericentriolar Golgi stacks during mitosis.". Proc. Natl. Acad. Sci. U.S.A. 98 (16): 9128–32. doi:10.1073/pnas.161283998. PMC 55384. PMID 11447294. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=55384.
Ward TH, Polishchuk RS, Caplan S, et al. (2001). "Maintenance of Golgi structure and function depends on the integrity of ER export.". J. Cell Biol. 155 (4): 557–70. doi:10.1083/jcb.200107045. PMC 2198855. PMID 11706049. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2198855.
Short B, Preisinger C, Körner R, et al. (2002). "A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic.". J. Cell Biol. 155 (6): 877–83. doi:10.1083/jcb.200108079. PMC 2150909. PMID 11739401. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2150909.
Barr FA, Preisinger C, Kopajtich R, Körner R (2002). "Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus.". J. Cell Biol. 155 (6): 885–91. doi:10.1083/jcb.200108102. PMC 2150891. PMID 11739402. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2150891.
Marra P, Maffucci T, Daniele T, et al. (2002). "The GM130 and GRASP65 Golgi proteins cycle through and define a subdomain of the intermediate compartment.". Nat. Cell Biol. 3 (12): 1101–13. doi:10.1038/ncb1201-1101. PMID 11781572.
Lane JD, Lucocq J, Pryde J, et al. (2002). "Caspase-mediated cleavage of the stacking protein GRASP65 is required for Golgi fragmentation during apoptosis.". J. Cell Biol. 156 (3): 495–509. doi:10.1083/jcb.200110007. PMC 2173349. PMID 11815631. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2173349.
Jesch SA (2002). "Inheriting a structural scaffold for Golgi biosynthesis.". Bioessays 24 (7): 584–7. doi:10.1002/bies.10122. PMID 12111718.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Protopopov A, Kashuba V, Zabarovska VI, et al. (2003). "An integrated physical and gene map of the 3.5-Mb chromosome 3p21.3 (AP20) region implicated in major human epithelial malignancies.". Cancer Res. 63 (2): 404–12. PMID 12543795.
Wang Y, Seemann J, Pypaert M, et al. (2003). "A direct role for GRASP65 as a mitotically regulated Golgi stacking factor.". EMBO J. 22 (13): 3279–90. doi:10.1093/emboj/cdg317. PMC 165642. PMID 12839990. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=165642.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Colland F, Jacq X, Trouplin V, et al. (2004). "Functional proteomics mapping of a human signaling pathway.". Genome Res. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC 442148. PMID 15231748. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=442148.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Wang Y, Satoh A, Warren G (2005). "Mapping the functional domains of the Golgi stacking factor GRASP65.". J. Biol. Chem. 280 (6): 4921–8. doi:10.1074/jbc.M412407200. PMID 15576368.
Yoshimura S, Yoshioka K, Barr FA, et al. (2005). "Convergence of cell cycle regulation and growth factor signals on GRASP65.". J. Biol. Chem. 280 (24): 23048–56. doi:10.1074/jbc.M502442200. PMID 15834132.
Sütterlin C, Polishchuk R, Pecot M, Malhotra V (2005). "The Golgi-associated protein GRASP65 regulates spindle dynamics and is essential for cell division.". Mol. Biol. Cell 16 (7): 3211–22. doi:10.1091/mbc.E04-12-1065. PMC 1165405. PMID 15888544. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1165405.

GORASP1

Interactions

References

Further reading