GABARAP
Gamma-aminobutyric acid receptor-associated protein is a protein that in humans is encoded by the GABARAP gene.[1]
Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.[2]
A study has screened 2000 participants representing more than 600 families of smokers and examined different genes showing linkage to nicotine addiction. Some of these showing linkage are the GABA-B receptor subunit 2 (GABAB2) gene on chromosome 9, and the GABA-A receptor-associated protein (GABARAP) gene on chromosome 17.[3]
Interactions
GABARAP has been shown to interact with TFRC,[4] ULK1[5][6] and GABRG2.[1][7][8]
References
- ^ a b Wang H, Bedford FK, Brandon NJ, Moss SJ, Olsen RW (February 1999). "GABA(A)-receptor-associated protein links GABA(A) receptors and the cytoskeleton". Nature 397 (6714): 69–72. doi:10.1038/16264. PMID 9892355.
- ^ "Entrez Gene: GABARAP GABA(A) receptor-associated protein". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=11337.
- ^ "Nicotine Addiction Might Be Controlled By Influencing Brain Mechanisms". 2007-12-10. http://www.sciencedaily.com/releases/2007/12/071208092505.htm.
- ^ Green, Frank; O'Hare Thomas, Blackwell Aaron, Enns Caroline A (May 2002). "Association of human transferrin receptor with GABARAP". FEBS Lett. (Netherlands) 518 (1–3): 101–106. doi:10.1016/S0014-5793(02)02655-8. ISSN 0014-5793. PMID 11997026.
- ^ Ewing, Rob M; Chu Peter, Elisma Fred, Li Hongyan, Taylor Paul, Climie Shane, McBroom-Cerajewski Linda, Robinson Mark D, O'Connor Liam, Li Michael, Taylor Rod, Dharsee Moyez, Ho Yuen, Heilbut Adrian, Moore Lynda, Zhang Shudong, Ornatsky Olga, Bukhman Yury V, Ethier Martin, Sheng Yinglun, Vasilescu Julian, Abu-Farha Mohamed, Lambert Jean-Philippe, Duewel Henry S, Stewart Ian I, Kuehl Bonnie, Hogue Kelly, Colwill Karen, Gladwish Katharine, Muskat Brenda, Kinach Robert, Adams Sally-Lin, Moran Michael F, Morin Gregg B, Topaloglou Thodoros, Figeys Daniel (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. (England) 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1847948.
- ^ Okazaki, N; Yan J, Yuasa S, Ueno T, Kominami E, Masuho Y, Koga H, Muramatsu M (December 2000). "Interaction of the Unc-51-like kinase and microtubule-associated protein light chain 3 related proteins in the brain: possible role of vesicular transport in axonal elongation". Brain Res. Mol. Brain Res. (Netherlands) 85 (1–2): 1–12. doi:10.1016/S0169-328X(00)00218-7. ISSN 0169-328X. PMID 11146101.
- ^ Nymann-Andersen, Jesper; Wang Hongbing, Chen Li, Kittler Josef T, Moss Stephen J, Olsen Richard W (March 2002). "Subunit specificity and interaction domain between GABA(A) receptor-associated protein (GABARAP) and GABA(A) receptors". J. Neurochem. (England) 80 (5): 815–823. doi:10.1046/j.0022-3042.2002.00762.x. ISSN 0022-3042. PMID 11948245.
- ^ Coyle, Joseph E; Qamar Seema, Rajashankar Kanagalaghatta R, Nikolov Dimitar B (January2002). "Structure of GABARAP in two conformations: implications for GABA(A) receptor localization and tubulin binding". Neuron (United States) 33 (1): 63–74. doi:10.1016/S0896-6273(01)00558-X. ISSN 0896-6273. PMID 11779480.
Further reading
- Kanematsu T, Hirata M (2002). "[The analysis of protein-protein interaction with special reference to PRIP-1]". Nippon Yakurigaku Zasshi 119 (4): 241–6. doi:10.1254/fpj.119.241. PMID 11979730.
- Paz Y, Elazar Z, Fass D (2000). "Structure of GATE-16, membrane transport modulator and mammalian ortholog of autophagocytosis factor Aut7p". J. Biol. Chem. 275 (33): 25445–25450. doi:10.1074/jbc.C000307200. PMID 10856287.
- Wang H, Olsen RW (2000). "Binding of the GABA(A) receptor-associated protein (GABARAP) to microtubules and microfilaments suggests involvement of the cytoskeleton in GABARAPGABA(A) receptor interaction". J. Neurochem. 75 (2): 644–655. doi:10.1046/j.1471-4159.2000.0750644.x. PMID 10899939.
- Kneussel M, Haverkamp S, Fuhrmann JC et al. (2000). "The gamma-aminobutyric acid type A receptor (GABAAR)-associated protein GABARAP interacts with gephyrin but is not involved in receptor anchoring at the synapse". Proc. Natl. Acad. Sci. U.S.A. 97 (15): 8594–8599. doi:10.1073/pnas.97.15.8594. PMC 26993. PMID 10900017. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=26993.
- Hu RM, Han ZG, Song HD et al. (2000). "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning". Proc. Natl. Acad. Sci. U.S.A. 97 (17): 9543–9548. doi:10.1073/pnas.160270997. PMC 16901. PMID 10931946. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=16901.
- Chen L, Wang H, Vicini S, Olsen RW (2000). "The gamma-aminobutyric acid type A (GABAA) receptor-associated protein (GABARAP) promotes GABAA receptor clustering and modulates the channel kinetics". Proc. Natl. Acad. Sci. U.S.A. 97 (21): 11557–11562. doi:10.1073/pnas.190133497. PMC 17239. PMID 10984509. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=17239.
- Zhang QH, Ye M, Wu XY et al. (2001). "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells". Genome Res. 10 (10): 1546–1560. doi:10.1101/gr.140200. PMC 310934. PMID 11042152. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=310934.
- Okazaki N, Yan J, Yuasa S et al. (2001). "Interaction of the Unc-51-like kinase and microtubule-associated protein light chain 3 related proteins in the brain: possible role of vesicular transport in axonal elongation". Brain Res. Mol. Brain Res. 85 (1–2): 1–12. doi:10.1016/S0169-328X(00)00218-7. PMID 11146101.
- Xin Y, Yu L, Chen Z et al. (2001). "Cloning, expression patterns, and chromosome localization of three human and two mouse homologues of GABA(A) receptor-associated protein". Genomics 74 (3): 408–413. doi:10.1006/geno.2001.6555. PMID 11414770.
- Kittler JT, Rostaing P, Schiavo G et al. (2001). "The subcellular distribution of GABARAP and its ability to interact with NSF suggest a role for this protein in the intracellular transport of GABA(A) receptors". Mol. Cell. Neurosci. 18 (1): 13–25. doi:10.1006/mcne.2001.1005. PMID 11461150.
- Stangler T, Mayr LM, Dingley AJ et al. (2002). "Sequence-specific 1H, 13C and 15N resonance assignments of human GABA receptor associated protein". J. Biomol. NMR 21 (2): 183–4. doi:10.1023/A:1012416810974. PMID 11727985.
- Harris R, McAlister MS, Sankar A et al. (2002). "Backbone 1H, 13C, and 15N resonance assignments for a 14 kD protein, GABA(A) receptor associated protein (GABARAP)". J. Biomol. NMR 21 (2): 185–6. doi:10.1023/A:1012473609468. PMID 11727986.
- Knight D, Harris R, McAlister MS et al. (2002). "The X-ray crystal structure and putative ligand-derived peptide binding properties of gamma-aminobutyric acid receptor type A receptor-associated protein". J. Biol. Chem. 277 (7): 5556–5561. doi:10.1074/jbc.M109753200. PMID 11729197.
- Coyle JE, Qamar S, Rajashankar KR, Nikolov DB (2002). "Structure of GABARAP in two conformations: implications for GABA(A) receptor localization and tubulin binding". Neuron 33 (1): 63–74. doi:10.1016/S0896-6273(01)00558-X. PMID 11779480.
- Tanida I, Tanida-Miyake E, Komatsu M et al. (2002). "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p". J. Biol. Chem. 277 (16): 13739–13744. doi:10.1074/jbc.M200385200. PMID 11825910.
- Stangler T, Mayr LM, Willbold D (2002). "Solution structure of human GABA(A) receptor-associated protein GABARAP: implications for biological function and its regulation". J. Biol. Chem. 277 (16): 13363–13366. doi:10.1074/jbc.C200050200. PMID 11875056.
- Kouno T, Miura K, Kanematsu T et al. (2003). "1H, 13C and '5N resonance assignments of GABARAP, GABAA receptor associated protein". J. Biomol. NMR 22 (1): 97–98. doi:10.1023/A:1013884402033. PMID 11885988.
- Tanida I, Tanida-Miyake E, Nishitani T et al. (2002). "Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs". Biochem. Biophys. Res. Commun. 292 (1): 256–262. doi:10.1006/bbrc.2002.6645. PMID 11890701.
- Nymann-Andersen J, Wang H, Chen L et al. (2002). "Subunit specificity and interaction domain between GABA(A) receptor-associated protein (GABARAP) and GABA(A) receptors". J. Neurochem. 80 (5): 815–823. doi:10.1046/j.0022-3042.2002.00762.x. PMID 11948245.
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PDB gallery
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1gnu: GABA(A) RECEPTOR ASSOCIATED PROTEIN GABARAP
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1kjt: Crystal Structure of the GABA(A) Receptor Associated Protein, GABARAP
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1klv: Solution Structure and Backbone Dynamics of GABARAP, GABAA Receptor associated protein
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1km7: Solution Structure and Backbone Dynamics of GABARAP, GABAA Receptor Associated Protein
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1kot: Solution Structure of Human GABA Receptor Associated Protein GABARAP
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