Far upstream element-binding protein 1

Far upstream element (FUSE) binding protein 1

PDB rendering based on 1j4w.

Available structures
PDB	1J4W, 2KXH

Identifiers

Symbols

FUBP1; FBP; FUBP

External IDs

OMIM: 603444 MGI: 1196294 HomoloGene: 48253 GeneCards: FUBP1 Gene

Gene Ontology
Molecular function	• DNA binding • single-stranded DNA binding • sequence-specific DNA binding transcription factor activity • RNA binding • protein binding
Cellular component	• nucleus • nucleolus
Biological process	• regulation of transcription, DNA-dependent • transcription from RNA polymerase II promoter
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 1:
78.41 – 78.44 Mb

Chr 3:
151.87 – 151.9 Mb

PubMed search

[1]

[2]

Far upstream element-binding protein 1 is a protein that in humans is encoded by the FUBP1 gene.^[1]^[2]

This gene encodes a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase.^[2]

Interactions

Far upstream element-binding protein 1 has been shown to interact with MAPK14^[3] and SMN1.^[4]

References

^ Duncan R, Bazar L, Michelotti G, Tomonaga T, Krutzsch H, Avigan M, Levens D (Apr 1994). "A sequence-specific, single-strand binding protein activates the far upstream element of c-myc and defines a new DNA-binding motif". Genes Dev 8 (4): 465–80. doi:10.1101/gad.8.4.465. PMID 8125259.
^ ^a ^b "Entrez Gene: FUBP1 far upstream element (FUSE) binding protein 1". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=8880.
^ Kim, Min Jung; Park Bum-Joon, Kang Young-Sun, Kim Hyoung June, Park Jae-Hyun, Kang Jung Woo, Lee Sang Won, Han Jung Min, Lee Han-Woong, Kim Sunghoon (Jul. 2003). "Downregulation of FUSE-binding protein and c-myc by tRNA synthetase cofactor p38 is required for lung cell differentiation". Nat. Genet. (United States) 34 (3): 330–6. doi:10.1038/ng1182. ISSN 1061-4036. PMID 12819782.
^ Williams, B Y; Hamilton S L, Sarkar H K (Mar. 2000). "The survival motor neuron protein interacts with the transactivator FUSE binding protein from human fetal brain". FEBS Lett. (NETHERLANDS) 470 (2): 207–10. doi:10.1016/S0014-5793(00)01320-X. ISSN 0014-5793. PMID 10734235.

Williams BY, Hamilton SL, Sarkar HK (2000). "The survival motor neuron protein interacts with the transactivator FUSE binding protein from human fetal brain". FEBS Lett. 470 (2): 207–10. doi:10.1016/S0014-5793(00)01320-X. PMID 10734235.
Liu J, He L, Collins I, et al. (2000). "The FBP interacting repressor targets TFIIH to inhibit activated transcription". Mol. Cell 5 (2): 331–41. doi:10.1016/S1097-2765(00)80428-1. PMID 10882074.
Markovtsov V, Nikolic JM, Goldman JA, et al. (2000). "Cooperative assembly of an hnRNP complex induced by a tissue-specific homolog of polypyrimidine tract binding protein". Mol. Cell. Biol. 20 (20): 7463–79. doi:10.1128/MCB.20.20.7463-7479.2000. PMC 86300. PMID 11003644. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=86300.
He L, Weber A, Levens D (2000). "Nuclear targeting determinants of the far upstream element binding protein, a c-myc transcription factor". Nucleic Acids Res. 28 (22): 4558–65. doi:10.1093/nar/28.22.4558. PMC 113884. PMID 11071946. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=113884.
Vindigni A, Ochem A, Triolo G, Falaschi A (2001). "Identification of human DNA helicase V with the far upstream element-binding protein". Nucleic Acids Res. 29 (5): 1061–7. doi:10.1093/nar/29.5.1061. PMC 29729. PMID 11222755. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=29729.
Liu J, Akoulitchev S, Weber A, et al. (2001). "Defective interplay of activators and repressors with TFIH in xeroderma pigmentosum". Cell 104 (3): 353–63. doi:10.1016/S0092-8674(01)00223-9. PMID 11239393.
Braddock DT, Louis JM, Baber JL, et al. (2002). "Structure and dynamics of KH domains from FBP bound to single-stranded DNA". Nature 415 (6875): 1051–6. doi:10.1038/4151051a. PMID 11875576.
Rappsilber J, Ryder U, Lamond AI, Mann M (2002). "Large-scale proteomic analysis of the human spliceosome". Genome Res. 12 (8): 1231–45. doi:10.1101/gr.473902. PMC 186633. PMID 12176931. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=186633.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Kim MJ, Park BJ, Kang YS, et al. (2003). "Downregulation of FUSE-binding protein and c-myc by tRNA synthetase cofactor p38 is required for lung cell differentiation". Nat. Genet. 34 (3): 330–6. doi:10.1038/ng1182. PMID 12819782.
Brandenberger R, Wei H, Zhang S, et al. (2005). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation". Nat. Biotechnol. 22 (6): 707–16. doi:10.1038/nbt971. PMID 15146197.
Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=514446.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Kouzine F, Liu J, Sanford S, et al. (2004). "The dynamic response of upstream DNA to transcription-generated torsional stress". Nat. Struct. Mol. Biol. 11 (11): 1092–100. doi:10.1038/nsmb848. PMID 15502847.
Rush J, Moritz A, Lee KA, et al. (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455.
Stelzl U, Worm U, Lalowski M, et al. (2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. PMID 16169070.
Fautsch MP, Vrabel AM, Johnson DH (2006). "The identification of myocilin-associated proteins in the human trabecular meshwork". Exp. Eye Res. 82 (6): 1046–52. doi:10.1016/j.exer.2005.09.016. PMID 16289162.
Liu J, Kouzine F, Nie Z, et al. (2006). "The FUSE/FBP/FIR/TFIIH system is a molecular machine programming a pulse of c-myc expression". EMBO J. 25 (10): 2119–30. doi:10.1038/sj.emboj.7601101. PMC 1462968. PMID 16628215. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1462968.
Ko HS, Kim SW, Sriram SR, et al. (2006). "Identification of far upstream element-binding protein-1 as an authentic Parkin substrate". J. Biol. Chem. 281 (24): 16193–6. doi:10.1074/jbc.C600041200. PMID 16672220.

PDB gallery

1j4w: COMPLEX OF THE KH3 and KH4 DOMAINS OF FBP WITH A SINGLE_STRANDED 29mer DNA OLIGONUCLEOTIDE FROM THE FUSE ELEMENT OF THE C-MYC ONCOGENE

1x4m: Solution structure of KH domain in Far upstream element binding protein 1

1x4n: Solution structure of KH domain in FUSE binding protein 1

Far upstream element-binding protein 1

Interactions

References

Further reading