DDX3X

DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked

PDB rendering based on 2i4i.

Available structures
PDB	2I4I, 2JGN, 3JRV

Identifiers

Symbols

DDX3X; DBX; DDX14; DDX3; HLP2

External IDs

OMIM: 300160 MGI: 103064 HomoloGene: 3425 GeneCards: DDX3X Gene

EC number

3.6.4.13

Gene Ontology
Molecular function	• nucleotide binding • DNA binding • RNA binding • ATP-dependent RNA helicase activity • helicase activity • protein binding • ATP binding • hydrolase activity
Cellular component	• nucleus • cytoplasm • nuclear speck
Biological process	• interspecies interaction between organisms
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr X:
41.19 – 41.22 Mb

Chr X:
12.86 – 12.87 Mb

PubMed search

[1]

[2]

ATP-dependent RNA helicase DDX3X is an enzyme that in humans is encoded by the DDX3X gene.^[1]^[2]^[3]

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. This gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog.^[3]

References

^ Lahn BT, Page DC (Nov 1997). "Functional coherence of the human Y chromosome". Science 278 (5338): 675–680. doi:10.1126/science.278.5338.675. PMID 9381176.
^ Park SH, Lee SG, Kim Y, Song K (Oct 1998). "Assignment of a human putative RNA helicase gene, DDX3, to human X chromosome bands p11.3→p11.23". Cytogenet Cell Genet 81 (3–4): 178–179. doi:10.1159/000015022. PMID 9730595.
^ ^a ^b "Entrez Gene: DDX3X DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=1654.

Li L, Li HS, Pauza CD et al. (2006). "Roles of HIV-1 auxiliary proteins in viral pathogenesis and host-pathogen interactions". Cell Res. 15 (11–12): 923–934. doi:10.1038/sj.cr.7290370. PMID 16354571.
Owsianka AM, Patel AH (1999). "Hepatitis C virus core protein interacts with a human DEAD box protein DDX3". Virology 257 (2): 330–340. doi:10.1006/viro.1999.9659. PMID 10329544.
Mamiya N, Worman HJ (1999). "Hepatitis C virus core protein binds to a DEAD box RNA helicase". J. Biol. Chem. 274 (22): 15751–15756. doi:10.1074/jbc.274.22.15751. PMID 10336476.
Yagüe J, Alvarez I, Rognan D et al. (2000). "An N-acetylated natural ligand of human histocompatibility leukocyte antigen (HLA)-B39. Classical major histocompatibility complex class I proteins bind peptides with a blocked NH(2) terminus in vivo". J. Exp. Med. 191 (12): 2083–2092. doi:10.1084/jem.191.12.2083. PMC 2193201. PMID 10859333. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2193201.
Kim YS, Lee SG, Park SH, Song K (2002). "Gene structure of the human DDX3 and chromosome mapping of its related sequences". Mol. Cells 12 (2): 209–14. PMID 11710523.
Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–16903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Li J, Hawkins IC, Harvey CD et al. (2003). "Regulation of alternative splicing by SRrp86 and its interacting proteins". Mol. Cell. Biol. 23 (21): 7437–7447. doi:10.1128/MCB.23.21.7437-7447.2003. PMC 207616. PMID 14559993. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=207616.
Shu H, Chen S, Bi Q et al. (2004). "Identification of phosphoproteins and their phosphorylation sites in the WEHI-231 B lymphoma cell line". Mol. Cell Proteomics 3 (3): 279–286. doi:10.1074/mcp.D300003-MCP200. PMID 14729942.
Bouwmeester T, Bauch A, Ruffner H et al. (2004). "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway". Nat. Cell Biol. 6 (2): 97–105. doi:10.1038/ncb1086. PMID 14743216.
Gerhard DS, Wagner L, Feingold EA et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–2127. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Yedavalli VS, Neuveut C, Chi YH et al. (2004). "Requirement of DDX3 DEAD box RNA helicase for HIV-1 Rev-RRE export function". Cell 119 (3): 381–392. doi:10.1016/j.cell.2004.09.029. PMID 15507209.
Dayton AI (2006). "Within you, without you: HIV-1 Rev and RNA export". Retrovirology 1: 35. doi:10.1186/1742-4690-1-35. PMC 526764. PMID 15516266. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=526764.
Krishnan V, Zeichner SL (2006). "Alterations in the expression of DEAD-box and other RNA binding proteins during HIV-1 replication". Retrovirology 1: 42. doi:10.1186/1742-4690-1-42. PMC 543576. PMID 15588285. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=543576.
Rush J, Moritz A, Lee KA et al. (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455.
Tao WA, Wollscheid B, O'Brien R et al. (2005). "Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry". Nat. Methods 2 (8): 591–598. doi:10.1038/nmeth776. PMID 16094384.
Gevaert K, Staes A, Van Damme J et al. (2006). "Global phosphoproteome analysis on human HepG2 hepatocytes using reversed-phase diagonal LC". Proteomics 5 (14): 3589–3599. doi:10.1002/pmic.200401217. PMID 16097034.
Rual JF, Venkatesan K, Hao T et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–1178. doi:10.1038/nature04209. PMID 16189514.
Chang PC, Chi CW, Chau GY et al. (2006). "DDX3, a DEAD box RNA helicase, is deregulated in hepatitis virus-associated hepatocellular carcinoma and is involved in cell growth control". Oncogene 25 (14): 1991–2003. doi:10.1038/sj.onc.1209239. PMID 16301996.

PDB gallery

2i4i: Crystal Structure of human DEAD-box RNA helicase DDX3X

DDX3X

References

Further reading