Chimerin 2

Chimerin (chimaerin) 2

PDB rendering based on 1xa6.

Available structures
PDB	1xa6

Identifiers

Symbols

CHN2; ARHGAP3; BCH; MGC138360; RHOGAP3

External IDs

OMIM: 602857 MGI: 1917243 HomoloGene: 31213 GeneCards: CHN2 Gene

Gene Ontology
Molecular function	• SH3/SH2 adaptor activity • GTPase activator activity • metal ion binding
Cellular component	• cytosol • membrane
Biological process	• small GTPase mediated signal transduction • positive regulation of GTPase activity • regulation of small GTPase mediated signal transduction
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 7:
29.19 – 29.55 Mb

Chr 6:
54.15 – 54.23 Mb

PubMed search

[1]

[2]

Beta-chimaerin is a protein that in humans is encoded by the CHN2 gene.^[1]^[2]

This gene is a member of the chimerin family and encodes a protein with a phorbol-ester/DAG-type zinc finger, a Rho-GAP domain and an SH2 domain. This protein has GTPase-activating protein activity that is regulated by phospholipid binding and binding of diacylglycerol (DAG) induces translocation of the protein from the cytosol to the Golgi apparatus membrane. The protein plays a role in the proliferation and migration of smooth muscle cells. Decreased expression of this gene is associated with high-grade gliomas and breast tumors, and increased expression of this gene is associated with lymphomas. Mutations in this gene have been associated with schizophrenia in men. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.^[2]

References

^ Leung T, How BE, Manser E, Lim L (Jun 1994). "Cerebellar beta 2-chimaerin, a GTPase-activating protein for p21 ras-related rac is specifically expressed in granule cells and has a unique N-terminal SH2 domain". J Biol Chem 269 (17): 12888–92. PMID 8175705.
^ ^a ^b "Entrez Gene: CHN2 chimerin (chimaerin) 2". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=1124.

Yuan S, Miller DW, Barnett GH, et al. (1995). "Identification and characterization of human beta 2-chimaerin: association with malignant transformation in astrocytoma". Cancer Res. 55 (15): 3456–61. PMID 7614486.
Caloca MJ, Fernandez N, Lewin NE, et al. (1997). "Beta2-chimaerin is a high affinity receptor for the phorbol ester tumor promoters". J. Biol. Chem. 272 (42): 26488–96. doi:10.1074/jbc.272.42.26488. PMID 9334226.
Caloca MJ, Garcia-Bermejo ML, Blumberg PM, et al. (1999). "beta2-chimaerin is a novel target for diacylglycerol: binding properties and changes in subcellular localization mediated by ligand binding to its C1 domain". Proc. Natl. Acad. Sci. U.S.A. 96 (21): 11854–9. doi:10.1073/pnas.96.21.11854. PMC 18376. PMID 10518540. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=18376.
Caloca MJ, Wang H, Delemos A, et al. (2001). "Phorbol esters and related analogs regulate the subcellular localization of beta 2-chimaerin, a non-protein kinase C phorbol ester receptor". J. Biol. Chem. 276 (21): 18303–12. doi:10.1074/jbc.M011368200. PMID 11278894.
Wang H, Kazanietz MG (2002). "Chimaerins, novel non-protein kinase C phorbol ester receptors, associate with Tmp21-I (p23): evidence for a novel anchoring mechanism involving the chimaerin C1 domain". J. Biol. Chem. 277 (6): 4541–50. doi:10.1074/jbc.M107150200. PMID 11689559.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Scherer SW, Cheung J, MacDonald JR, et al. (2003). "Human chromosome 7: DNA sequence and biology". Science 300 (5620): 767–72. doi:10.1126/science.1083423. PMC 2882961. PMID 12690205. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2882961.
Nishiu M, Yanagawa R, Nakatsuka S, et al. (2003). "Microarray analysis of gene-expression profiles in diffuse large B-cell lymphoma: identification of genes related to disease progression". Jpn. J. Cancer Res. 93 (8): 894–901. PMID 12716467.
Hillier LW, Fulton RS, Fulton LA, et al. (2003). "The DNA sequence of human chromosome 7". Nature 424 (6945): 157–64. doi:10.1038/nature01782. PMID 12853948.
Caloca MJ, Wang H, Kazanietz MG (2004). "Characterization of the Rac-GAP (Rac-GTPase-activating protein) activity of beta2-chimaerin, a 'non-protein kinase C' phorbol ester receptor". Biochem. J. 375 (Pt 2): 313–21. doi:10.1042/BJ20030727. PMC 1223697. PMID 12877655. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1223697.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Canagarajah B, Leskow FC, Ho JY, et al. (2004). "Structural mechanism for lipid activation of the Rac-specific GAP, beta2-chimaerin". Cell 119 (3): 407–18. doi:10.1016/j.cell.2004.10.012. PMID 15507211.
Hashimoto R, Yoshida M, Kunugi H, et al. (2005). "A missense polymorphism (H204R) of a Rho GTPase-activating protein, the chimerin 2 gene, is associated with schizophrenia in men". Schizophrenia Research 73 (2–3): 383–5. doi:10.1016/j.schres.2004.01.017. PMID 15653288.
Barrios-Rodiles M, Brown KR, Ozdamar B, et al. (2005). "High-throughput mapping of a dynamic signaling network in mammalian cells". Science 307 (5715): 1621–5. doi:10.1126/science.1105776. PMID 15761153.
Yang C, Liu Y, Leskow FC, et al. (2005). "Rac-GAP-dependent inhibition of breast cancer cell proliferation by {beta}2-chimerin.". J. Biol. Chem. 280 (26): 24363–70. doi:10.1074/jbc.M411629200. PMID 15863513.
Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1356129.
Siliceo M, García-Bernal D, Carrasco S, et al. (2006). "Beta2-chimaerin provides a diacylglycerol-dependent mechanism for regulation of adhesion and chemotaxis of T cells". J. Cell. Sci. 119 (Pt 1): 141–52. doi:10.1242/jcs.02722. PMID 16352660.
Yang C, Liu Y, Lemmon MA, Kazanietz MG (2006). "Essential role for Rac in heregulin beta1 mitogenic signaling: a mechanism that involves epidermal growth factor receptor and is independent of ErbB4". Mol. Cell. Biol. 26 (3): 831–42. doi:10.1128/MCB.26.3.831-842.2006. PMC 1347034. PMID 16428439. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1347034.

PDB gallery

1xa6: Crystal Structure of the Human Beta2-Chimaerin

GTP-binding protein regulators

GTPase activating protein

Monomeric	Chimerin (CHN1, CHN2) · RasGAP (NF1, IQGAP) · Tuberous sclerosis protein (TSC1, TSC2)

Heterotrimeric	Regulator of G protein signalling: RGS1, RGS2, RGS3, RGS4, RGS5, RGS6, RGS7, RGS8, RGS9, RGS10, RGS11, RGS12, RGS13, RGS14, RGS16, RGS17, RGS18, RGS19, RGS20, RGS21

Guanine nucleotide exchange factor

EIF2B · Son of Sevenless · Ras-GRF1

FGD: FGD1 · FGD2 · FGD3 · FGD4

ALS2 · SIL1 · IQSEC2

Other

Guanosine nucleotide dissociation inhibitors

B trdu: iter (nrpl/grfl/cytl/horl), csrc (lgic, enzr, gprc, igsr, intg, nrpr/grfr/cytr), itra (adap, gbpr, mapk), calc, lipd; path (hedp, wntp, tgfp+mapp, notp, jakp, fsap, hipp, tlrp)

Chimerin 2

References

Further reading