| Calcein | |
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| Identifiers | |
| CAS number | 1461-15-0 |
| PubChem | 65079 |
| ChemSpider | 58589 |
| ChEBI | CHEBI:51903 |
| Jmol-3D images | Image 1 Image 2 |
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| Properties | |
| Molecular formula | C30H26N2O13 |
| Molar mass | 622.55 g/mol |
| Melting point |
Decomposes |
| Boiling point |
N/A |
| (verify) (what is: /?) Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) |
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| Infobox references | |
Calcein, also known as fluorexon, fluorescein complex, is a fluorescent dye with an excitation and emission wavelengths of 495/515 nm, respectively. Calcein also self-quenches even at concentrations below 100mM[1]. It is used as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium. It has the appearance of orange crystals.
The acetomethoxy derivate of calcein (calcein AM) is used in biology as it can be transported through the cellular membrane into live cells, which makes it useful for testing of cell viability and for short-term labeling of cells. An acetomethoxy group obscures the part of the molecule that chelates Ca2+, Mg2+, Zn2+ and other ions. After transport into the cells, intracellular esterases remove the acetomethoxy group, the molecule gets trapped inside and gives out strong green fluorescence. As dead cells lack active esterases, only live cells are labeled[2] and counted by flow cytometry.
Calcein is now rarely used as a Ca2+ or Mg2+ indicator because its fluorescence is directly sensitive to these ions only at strongly alkaline pH, and thus it is not particularly useful for measuring Ca2+ or Mg2+ in cells. Fluorescence of calcein is quenched strongly by Co2+, Ni2+ and Cu2+ and appreciably by Fe3+ and Mn2+ at physiological pH. This fluorescence quenching response can be exploited for detecting the opening of the mitochondrial permeability transition pore. Calcein is commonly used for cell tracing and in studies of endocytosis and gap junctions.[3]
The acetoxymethyl ester of calcein is also used to detect drug interactions with multidrug resistance proteins (ABC transporters ATP-binding cassette transporter genes) in intact cells as it is an excellent substrate of the multidrug resistance transporter 1 (MDR1) P-glycoprotein and the de:Multidrug Resistance-Related Protein (MRP1).[4] The calcein AM assay can be used as a model for drug-drug interactions, for screening transporter substrates and/or inhibitors;[5] and also to determine in vitro drug resistance of cells, including samples from patients.[6][7]
Calcein is also used for marking freshly hatched fish[8] and for labeling of bones in live animals.