CUL3

Cullin 3

PDB rendering based on 1iuy.

Available structures
PDB	1iuy

Identifiers

External IDs

OMIM: 603136 MGI: 1347360 HomoloGene: 2661 GeneCards: CUL3 Gene

Gene Ontology
Molecular function	• ubiquitin-protein ligase activity • protein binding • cyclin binding • ubiquitin protein ligase binding
Cellular component	• nucleus • Golgi apparatus • polar microtubule • Cul3-RING ubiquitin ligase complex
Biological process	• G1/S transition of mitotic cell cycle • mitotic anaphase • cell morphogenesis • cytokinesis • in utero embryonic development • trophectodermal cellular morphogenesis • cell cycle arrest • gastrulation • cyclin catabolic process • positive regulation of cell proliferation • induction of apoptosis by intracellular signals • Wnt receptor signaling pathway • cell migration • protein ubiquitination • negative regulation of Rho protein signal transduction • stress fiber assembly • proteasomal ubiquitin-dependent protein catabolic process • anaphase • cellular response to protein stimulus
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

ENSG00000036257

ENSMUSG00000004364

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 2:
225.33 – 225.45 Mb

Chr 1:
80.26 – 80.34 Mb

PubMed search

Cullin-3 is a protein that in humans is encoded by the CUL3 gene.^[1]^[2]^[3]

Interactions

CUL3 has been shown to interact with KLHL12,^[4] CAND1,^[5] KEAP1,^[6]^[7] DCUN1D1^[8] and Cyclin E1.^[9]

References

^ Kipreos ET, Lander LE, Wing JP, He WW, Hedgecock EM (Aug 1996). "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family". Cell 85 (6): 829–39. doi:10.1016/S0092-8674(00)81267-2. PMID 8681378.
^ Wimuttisuk W, Singer JD (Feb 2007). "The Cullin3 Ubiquitin Ligase Functions as a Nedd8-bound Heterodimer". Mol Biol Cell 18 (3): 899–909. doi:10.1091/mbc.E06-06-0542. PMC 1805106. PMID 17192413. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1805106.
^ "Entrez Gene: CUL3 cullin 3". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=8452.
^ Rondou, Pieter; Haegeman Guy, Vanhoenacker Peter, Van Craenenbroeck Kathleen (Apr. 2008). "BTB Protein KLHL12 Targets the Dopamine D4 Receptor for Ubiquitination by a Cul3-based E3 Ligase". J. Biol. Chem. (United States) 283 (17): 11083–96. doi:10.1074/jbc.M708473200. ISSN 0021-9258. PMC 2431063. PMID 18303015. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2431063.
^ Min, Kyoeng-Woo; Hwang Ji-Won, Lee Jong-Sik, Park Yoon, Tamura Taka-aki, Yoon Jong-Bok (May. 2003). "TIP120A associates with cullins and modulates ubiquitin ligase activity". J. Biol. Chem. (United States) 278 (18): 15905–10. doi:10.1074/jbc.M213070200. ISSN 0021-9258. PMID 12609982.
^ Wang, Xiao-Jun; Sun Zheng, Chen Weimin, Li Yanjie, Villeneuve Nicole F, Zhang Donna D (Aug. 2008). "Activation of Nrf2 by arsenite and monomethylarsonous acid is independent of Keap1-C151: enhanced Keap1-Cul3 interaction". Toxicol. Appl. Pharmacol. (United States) 230 (3): 383–9. doi:10.1016/j.taap.2008.03.003. ISSN 0041-008X. PMC 2610481. PMID 18417180. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2610481.
^ Rachakonda, Girish; Xiong Ying, Sekhar Konjeti R, Stamer Sheryl L, Liebler Daniel C, Freeman Michael L (Mar. 2008). "Covalent modification at Cys151 dissociates the electrophile sensor Keap1 from the ubiquitin ligase CUL3". Chem. Res. Toxicol. (United States) 21 (3): 705–10. doi:10.1021/tx700302s. ISSN 0893-228X. PMID 18251510.
^ Kim, Alexander Y; Bommeljé Claire C, Lee Benjamin E, Yonekawa Yoshihiro, Choi Lydia, Morris Luc G, Huang Guochang, Kaufman Andrew, Ryan Russel J H, Hao Bing, Ramanathan Y, Singh Bhuvanesh (Nov. 2008). "SCCRO (DCUN1D1) Is an Essential Component of the E3 Complex for Neddylation". J. Biol. Chem. (United States) 283 (48): 33211–20. doi:10.1074/jbc.M804440200. ISSN 0021-9258. PMC 2586271. PMID 18826954. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2586271.
^ Singer, J D; Gurian-West M, Clurman B, Roberts J M (Sep. 1999). "Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells". Genes Dev. (UNITED STATES) 13 (18): 2375–87. doi:10.1101/gad.13.18.2375. ISSN 0890-9369. PMC 317026. PMID 10500095. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=317026.

Further reading

Andersson B, Wentland MA, Ricafrente JY, et al. (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
Yu W, Andersson B, Worley KC, et al. (1997). "Large-Scale Concatenation cDNA Sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139146.
Michel JJ, Xiong Y (1998). "Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A". Cell Growth Differ. 9 (6): 435–49. PMID 9663463.
Du M, Sansores-Garcia L, Zu Z, Wu KK (1998). "Cloning and expression analysis of a novel salicylate suppressible gene, Hs-CUL-3, a member of cullin/Cdc53 family". J. Biol. Chem. 273 (38): 24289–92. doi:10.1074/jbc.273.38.24289. PMID 9733711.
Ishikawa K, Nagase T, Suyama M, et al. (1998). "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 5 (3): 169–76. doi:10.1093/dnares/5.3.169. PMID 9734811.
Ohta T, Michel JJ, Schottelius AJ, Xiong Y (1999). "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity". Mol. Cell 3 (4): 535–41. doi:10.1016/S1097-2765(00)80482-7. PMID 10230407.
Singer JD, Gurian-West M, Clurman B, Roberts JM (1999). "Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells". Genes Dev. 13 (18): 2375–87. doi:10.1101/gad.13.18.2375. PMC 317026. PMID 10500095. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=317026.
Hori T, Osaka F, Chiba T, et al. (2000). "Covalent modification of all members of human cullin family proteins by NEDD8". Oncogene 18 (48): 6829–34. doi:10.1038/sj.onc.1203093. PMID 10597293.
Maeda I, Ohta T, Koizumi H, Fukuda M (2001). "In vitro ubiquitination of cyclin D1 by ROC1-CUL1 and ROC1-CUL3". FEBS Lett. 494 (3): 181–5. doi:10.1016/S0014-5793(01)02343-2. PMID 11311237.
Lyapina S, Cope G, Shevchenko A, et al. (2001). "Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome". Science 292 (5520): 1382–5. doi:10.1126/science.1059780. PMID 11337588.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Min KW, Hwang JW, Lee JS, et al. (2003). "TIP120A associates with cullins and modulates ubiquitin ligase activity". J. Biol. Chem. 278 (18): 15905–10. doi:10.1074/jbc.M213070200. PMID 12609982.
Kobayashi A, Kang MI, Okawa H, et al. (2004). "Oxidative Stress Sensor Keap1 Functions as an Adaptor for Cul3-Based E3 Ligase To Regulate Proteasomal Degradation of Nrf2". Mol. Cell. Biol. 24 (16): 7130–9. doi:10.1128/MCB.24.16.7130-7139.2004. PMC 479737. PMID 15282312. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=479737.
Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=514446.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Rush J, Moritz A, Lee KA, et al. (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455.
Furukawa M, Xiong Y (2005). "BTB Protein Keap1 Targets Antioxidant Transcription Factor Nrf2 for Ubiquitination by the Cullin 3-Roc1 Ligase". Mol. Cell. Biol. 25 (1): 162–71. doi:10.1128/MCB.25.1.162-171.2005. PMC 538799. PMID 15601839. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=538799.
Hernández-Muñoz I, Lund AH, van der Stoop P, et al. (2005). "Stable X chromosome inactivation involves the PRC1 Polycomb complex and requires histone MACROH2A1 and the CULLIN3/SPOP ubiquitin E3 ligase". Proc. Natl. Acad. Sci. U.S.A. 102 (21): 7635–40. doi:10.1073/pnas.0408918102. PMC 1140410. PMID 15897469. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1140410.
Lu L, Zhou ZM, Huang XY, et al. (2005). "Identification and characterization of cul-3b, a novel hominine CUL-3 transcript variant". Asian J. Androl. 7 (2): 205–11. doi:10.1111/j.1745-7262.2005.00024.x. PMID 15897978.

PDB gallery

1iuy: Solution structure of the cullin-3 homologue