CUL2

Cullin 2

Identifiers

CUL2; MGC131970

External IDs

OMIM: 603135 MGI: 1918995 HomoloGene: 2662 GeneCards: CUL2 Gene

Gene Ontology
Molecular function	• protein binding • ubiquitin protein ligase binding
Cellular component	• cullin-RING ubiquitin ligase complex
Biological process	• G1/S transition of mitotic cell cycle • ubiquitin-dependent protein catabolic process • cell cycle arrest • negative regulation of cell proliferation • induction of apoptosis by intracellular signals • protein catabolic process • interspecies interaction between organisms
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

ENSG00000108094

ENSMUSG00000024231

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 10:
35.3 – 35.38 Mb

Chr 18:
3.38 – 3.44 Mb

PubMed search

Cullin-2 is a protein that in humans is encoded by the CUL2 gene.^[1]^[2]

Interactions

CUL2 has been shown to interact with DCUN1D1,^[3] SAP130,^[4] CAND1,^[5]^[4] RBX1,^[6]^[7] TCEB2^[8]^[4] and Von Hippel-Lindau tumor suppressor.^[9]^[10]^[8]^[11]^[4]

References

^ Kipreos ET, Lander LE, Wing JP, He WW, Hedgecock EM (Aug 1996). "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family". Cell 85 (6): 829–39. doi:10.1016/S0092-8674(00)81267-2. PMID 8681378.
^ "Entrez Gene: CUL2 cullin 2". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=8453.
^ Kim, Alexander Y; Bommeljé Claire C, Lee Benjamin E, Yonekawa Yoshihiro, Choi Lydia, Morris Luc G, Huang Guochang, Kaufman Andrew, Ryan Russel J H, Hao Bing, Ramanathan Y, Singh Bhuvanesh (Nov. 2008). "SCCRO (DCUN1D1) is an essential component of the E3 complex for neddylation". J. Biol. Chem. (United States) 283 (48): 33211–20. doi:10.1074/jbc.M804440200. ISSN 0021-9258. PMC 2586271. PMID 18826954. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2586271.
^ ^a ^b ^c ^d Menon, Suchithra; Tsuge Tomohiko, Dohmae Naoshi, Takio Koji, Wei Ning (2008). "Association of SAP130/SF3b-3 with Cullin-RING ubiquitin ligase complexes and its regulation by the COP9 signalosome". BMC Biochem. (England) 9: 1. doi:10.1186/1471-2091-9-1. PMC 2265268. PMID 18173839. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2265268.
^ Min, Kyoeng-Woo; Hwang Ji-Won, Lee Jong-Sik, Park Yoon, Tamura Taka-aki, Yoon Jong-Bok (May. 2003). "TIP120A associates with cullins and modulates ubiquitin ligase activity". J. Biol. Chem. (United States) 278 (18): 15905–10. doi:10.1074/jbc.M213070200. ISSN 0021-9258. PMID 12609982.
^ Dias, Dora C; Dolios Georgia, Wang Rong, Pan Zhen-Qiang (Dec. 2002). "CUL7: A DOC domain-containing cullin selectively binds Skp1.Fbx29 to form an SCF-like complex". Proc. Natl. Acad. Sci. U.S.A. (United States) 99 (26): 16601–6. doi:10.1073/pnas.252646399. ISSN 0027-8424. PMC 139190. PMID 12481031. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139190.
^ Ohta, T; Michel J J, Schottelius A J, Xiong Y (Apr. 1999). "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity". Mol. Cell (UNITED STATES) 3 (4): 535–41. doi:10.1016/S1097-2765(00)80482-7. ISSN 1097-2765. PMID 10230407.
^ ^a ^b Kamura, T; Burian D, Yan Q, Schmidt S L, Lane W S, Querido E, Branton P E, Shilatifard A, Conaway R C, Conaway J W (Aug. 2001). "Muf1, a novel Elongin BC-interacting leucine-rich repeat protein that can assemble with Cul5 and Rbx1 to reconstitute a ubiquitin ligase". J. Biol. Chem. (United States) 276 (32): 29748–53. doi:10.1074/jbc.M103093200. ISSN 0021-9258. PMID 11384984.
^ Ewing, Rob M; Chu Peter, Elisma Fred, Li Hongyan, Taylor Paul, Climie Shane, McBroom-Cerajewski Linda, Robinson Mark D, O'Connor Liam, Li Michael, Taylor Rod, Dharsee Moyez, Ho Yuen, Heilbut Adrian, Moore Lynda, Zhang Shudong, Ornatsky Olga, Bukhman Yury V, Ethier Martin, Sheng Yinglun, Vasilescu Julian, Abu-Farha Mohamed, Lambert Jean-Philippe, Duewel Henry S, Stewart Ian I, Kuehl Bonnie, Hogue Kelly, Colwill Karen, Gladwish Katharine, Muskat Brenda, Kinach Robert, Adams Sally-Lin, Moran Michael F, Morin Gregg B, Topaloglou Thodoros, Figeys Daniel (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. (England) 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1847948.
^ Ohh, M; Takagi Y, Aso T, Stebbins C E, Pavletich N P, Zbar B, Conaway R C, Conaway J W, Kaelin W G (Dec. 1999). "Synthetic peptides define critical contacts between elongin C, elongin B, and the von Hippel-Lindau protein". J. Clin. Invest. (UNITED STATES) 104 (11): 1583–91. doi:10.1172/JCI8161. ISSN 0021-9738. PMC 481054. PMID 10587522. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=481054.
^ Hacker, Kathryn E; Lee Caroline Martz, Rathmell W Kimryn (2008). Zhang, Baohong. ed. "VHL type 2B mutations retain VBC complex form and function". PLoS ONE (United States) 3 (11): e3801. doi:10.1371/journal.pone.0003801. PMC 2583047. PMID 19030229. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2583047.

Further reading

Kibel A, Iliopoulos O, DeCaprio JA, Kaelin WG (1995). "Binding of the von Hippel-Lindau tumor suppressor protein to Elongin B and C". Science 269 (5229): 1444–6. doi:10.1126/science.7660130. PMID 7660130.
Pause A, Lee S, Worrell RA, et al. (1997). "The von Hippel-Lindau tumor-suppressor gene product forms a stable complex with human CUL-2, a member of the Cdc53 family of proteins". Proc. Natl. Acad. Sci. U.S.A. 94 (6): 2156–61. doi:10.1073/pnas.94.6.2156. PMC 20057. PMID 9122164. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=20057.
Lonergan KM, Iliopoulos O, Ohh M, et al. (1998). "Regulation of hypoxia-inducible mRNAs by the von Hippel-Lindau tumor suppressor protein requires binding to complexes containing elongins B/C and Cul2". Mol. Cell. Biol. 18 (2): 732–41. PMC 108784. PMID 9447969. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=108784.
Wada H, Yeh ET, Kamitani T (1999). "Identification of NEDD8-conjugation site in human cullin-2". Biochem. Biophys. Res. Commun. 257 (1): 100–5. doi:10.1006/bbrc.1999.0339. PMID 10092517.
Ohta T, Michel JJ, Schottelius AJ, Xiong Y (1999). "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity". Mol. Cell 3 (4): 535–41. doi:10.1016/S1097-2765(00)80482-7. PMID 10230407.
Pause A, Peterson B, Schaffar G, et al. (1999). "Studying interactions of four proteins in the yeast two-hybrid system: structural resemblance of the pVHL/elongin BC/hCUL-2 complex with the ubiquitin ligase complex SKP1/cullin/F-box protein". Proc. Natl. Acad. Sci. U.S.A. 96 (17): 9533–8. doi:10.1073/pnas.96.17.9533. PMC 22243. PMID 10449727. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=22243.
Iwai K, Yamanaka K, Kamura T, et al. (1999). "Identification of the von Hippel-lindau tumor-suppressor protein as part of an active E3 ubiquitin ligase complex". Proc. Natl. Acad. Sci. U.S.A. 96 (22): 12436–41. doi:10.1073/pnas.96.22.12436. PMC 22941. PMID 10535940. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=22941.
Hori T, Osaka F, Chiba T, et al. (2000). "Covalent modification of all members of human cullin family proteins by NEDD8". Oncogene 18 (48): 6829–34. doi:10.1038/sj.onc.1203093. PMID 10597293.
Kamura T, Sato S, Iwai K, et al. (2000). "Activation of HIF1alpha ubiquitination by a reconstituted von Hippel-Lindau (VHL) tumor suppressor complex". Proc. Natl. Acad. Sci. U.S.A. 97 (19): 10430–5. doi:10.1073/pnas.190332597. PMC 27041. PMID 10973499. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=27041.
Lyapina S, Cope G, Shevchenko A, et al. (2001). "Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome". Science 292 (5520): 1382–5. doi:10.1126/science.1059780. PMID 11337588.
Kamura T, Burian D, Yan Q, et al. (2001). "Muf1, a novel Elongin BC-interacting leucine-rich repeat protein that can assemble with Cul5 and Rbx1 to reconstitute a ubiquitin ligase". J. Biol. Chem. 276 (32): 29748–53. doi:10.1074/jbc.M103093200. PMID 11384984.
Ohh M, Kim WY, Moslehi JJ, et al. (2002). "An intact NEDD8 pathway is required for Cullin-dependent ubiquitylation in mammalian cells". EMBO Rep. 3 (2): 177–82. doi:10.1093/embo-reports/kvf028. PMC 1083969. PMID 11818338. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1083969.
Min JH, Yang H, Ivan M, et al. (2002). "Structure of an HIF-1alpha -pVHL complex: hydroxyproline recognition in signaling". Science 296 (5574): 1886–9. doi:10.1126/science.1073440. PMID 12004076.
Brower CS, Sato S, Tomomori-Sato C, et al. (2002). "Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase". Proc. Natl. Acad. Sci. U.S.A. 99 (16): 10353–8. doi:10.1073/pnas.162424199. PMC 124918. PMID 12149480. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=124918.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Min KW, Hwang JW, Lee JS, et al. (2003). "TIP120A associates with cullins and modulates ubiquitin ligase activity". J. Biol. Chem. 278 (18): 15905–10. doi:10.1074/jbc.M213070200. PMID 12609982.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Deloukas P, Earthrowl ME, Grafham DV, et al. (2004). "The DNA sequence and comparative analysis of human chromosome 10". Nature 429 (6990): 375–81. doi:10.1038/nature02462. PMID 15164054.
Yan Q, Kamura T, Cai Y, et al. (2004). "Identification of Elongin C and Skp1 sequences that determine Cullin selection". J. Biol. Chem. 279 (41): 43019–26. doi:10.1074/jbc.M408018200. PMID 15280393.