CSTF2

Cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa

PDB rendering based on 1p1t.

Available structures
PDB	1P1T, 2J8P

Identifiers

Symbols

CSTF2; CstF-64

External IDs

OMIM: 600368 MGI: 1343054 HomoloGene: 80171 GeneCards: CSTF2 Gene

Gene Ontology
Molecular function	• nucleotide binding • RNA binding • protein binding
Cellular component	• nucleus • nucleoplasm • nucleolus • mRNA cleavage and polyadenylation specificity factor complex
Biological process	• nuclear mRNA splicing, via spliceosome • transcription from RNA polymerase II promoter • termination of RNA polymerase II transcription • mRNA polyadenylation • mRNA cleavage • mRNA processing • RNA splicing • gene expression • mRNA 3'-end processing
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr X:
100.08 – 100.1 Mb

Chr X:
130.59 – 130.62 Mb

PubMed search

[1]

[2]

Cleavage stimulation factor 64 kDa subunit is a protein that in humans is encoded by the CSTF2 gene.^[1]^[2]

This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs.^[2]

Interactions

CSTF2 has been shown to interact with CSTF3,^[3] SUB1,^[4] SYMPK,^[3] BARD1^[5]^[6] and BRCA1.^[5]^[6]

References

^ Takagaki Y, MacDonald CC, Shenk T, Manley JL (Mar 1992). "The human 64-kDa polyadenylylation factor contains a ribonucleoprotein-type RNA binding domain and unusual auxiliary motifs". Proc Natl Acad Sci U S A 89 (4): 1403–7. doi:10.1073/pnas.89.4.1403. PMC 48459. PMID 1741396. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=48459.
^ ^a ^b "Entrez Gene: CSTF2 cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=1478.
^ ^a ^b Takagaki, Y; Manley J L (Mar. 2000). "Complex protein interactions within the human polyadenylation machinery identify a novel component". Mol. Cell. Biol. (UNITED STATES) 20 (5): 1515–25. doi:10.1128/MCB.20.5.1515-1525.2000. ISSN 0270-7306. PMC 85326. PMID 10669729. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=85326.
^ Calvo, O; Manley J L (May. 2001). "Evolutionarily conserved interaction between CstF-64 and PC4 links transcription, polyadenylation, and termination". Mol. Cell (United States) 7 (5): 1013–23. doi:10.1016/S1097-2765(01)00236-2. ISSN 1097-2765. PMID 11389848.
^ ^a ^b Kleiman, F E; Manley J L (Mar. 2001). "The BARD1-CstF-50 interaction links mRNA 3' end formation to DNA damage and tumor suppression". Cell (United States) 104 (5): 743–53. doi:10.1016/S0092-8674(01)00270-7. ISSN 0092-8674. PMID 11257228.
^ ^a ^b Kleiman, F E; Manley J L (Sep. 1999). "Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50". Science (UNITED STATES) 285 (5433): 1576–9. doi:10.1126/science.285.5433.1576. ISSN 0036-8075. PMID 10477523.

Takagaki Y, Manley JL (1992). "A human polyadenylation factor is a G protein beta-subunit homologue.". J. Biol. Chem. 267 (33): 23471–4. PMID 1358884.
Murthy KG, Manley JL (1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation.". Genes Dev. 9 (21): 2672–83. doi:10.1101/gad.9.21.2672. PMID 7590244.
Takagaki Y, Manley JL (1994). "A polyadenylation factor subunit is the human homologue of the Drosophila suppressor of forked protein.". Nature 372 (6505): 471–4. doi:10.1038/372471a0. PMID 7984242.
Rüegsegger U, Beyer K, Keller W (1996). "Purification and characterization of human cleavage factor Im involved in the 3' end processing of messenger RNA precursors.". J. Biol. Chem. 271 (11): 6107–13. doi:10.1074/jbc.271.11.6107. PMID 8626397.
Schul W, Groenhout B, Koberna K, et al. (1996). "The RNA 3' cleavage factors CstF 64 kDa and CPSF 100 kDa are concentrated in nuclear domains closely associated with coiled bodies and newly synthesized RNA.". EMBO J. 15 (11): 2883–92. PMC 450226. PMID 8654386. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=450226.
Takagaki Y, Seipelt RL, Peterson ML, Manley JL (1997). "The polyadenylation factor CstF-64 regulates alternative processing of IgM heavy chain pre-mRNA during B cell differentiation.". Cell 87 (5): 941–52. doi:10.1016/S0092-8674(00)82000-0. PMID 8945520.
Takagaki Y, Manley JL (1997). "RNA recognition by the human polyadenylation factor CstF.". Mol. Cell. Biol. 17 (7): 3907–14. PMC 232243. PMID 9199325. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=232243.
Martincic K, Campbell R, Edwalds-Gilbert G, et al. (1998). "Increase in the 64-kDa subunit of the polyadenylation/cleavage stimulatory factor during the G0 to S phase transition.". Proc. Natl. Acad. Sci. U.S.A. 95 (19): 11095–100. doi:10.1073/pnas.95.19.11095. PMC 21601. PMID 9736695. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=21601.
Takagaki Y, Manley JL (1999). "Levels of polyadenylation factor CstF-64 control IgM heavy chain mRNA accumulation and other events associated with B cell differentiation.". Mol. Cell 2 (6): 761–71. doi:10.1016/S1097-2765(00)80291-9. PMID 9885564.
Kleiman FE, Manley JL (1999). "Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50.". Science 285 (5433): 1576–9. doi:10.1126/science.285.5433.1576. PMID 10477523.
Takagaki Y, Manley JL (2000). "Complex protein interactions within the human polyadenylation machinery identify a novel component.". Mol. Cell. Biol. 20 (5): 1515–25. doi:10.1128/MCB.20.5.1515-1525.2000. PMC 85326. PMID 10669729. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=85326.
Dass B, McMahon KW, Jenkins NA, et al. (2001). "The gene for a variant form of the polyadenylation protein CstF-64 is on chromosome 19 and is expressed in pachytene spermatocytes in mice.". J. Biol. Chem. 276 (11): 8044–50. doi:10.1074/jbc.M009091200. PMID 11113135.
Kleiman FE, Manley JL (2001). "The BARD1-CstF-50 interaction links mRNA 3' end formation to DNA damage and tumor suppression.". Cell 104 (5): 743–53. doi:10.1016/S0092-8674(01)00270-7. PMID 11257228.
Calvo O, Manley JL (2001). "Evolutionarily conserved interaction between CstF-64 and PC4 links transcription, polyadenylation, and termination.". Mol. Cell 7 (5): 1013–23. doi:10.1016/S1097-2765(01)00236-2. PMID 11389848.
Bléoo S, Sun X, Hendzel MJ, et al. (2002). "Association of human DEAD box protein DDX1 with a cleavage stimulation factor involved in 3'-end processing of pre-MRNA.". Mol. Biol. Cell 12 (10): 3046–59. PMC 60154. PMID 11598190. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=60154.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Pérez Cañadillas JM, Varani G (2003). "Recognition of GU-rich polyadenylation regulatory elements by human CstF-64 protein.". EMBO J. 22 (11): 2821–30. doi:10.1093/emboj/cdg259. PMC 156756. PMID 12773396. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=156756.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Xing H, Mayhew CN, Cullen KE, et al. (2004). "HSF1 modulation of Hsp70 mRNA polyadenylation via interaction with symplekin.". J. Biol. Chem. 279 (11): 10551–5. doi:10.1074/jbc.M311719200. PMID 14707147.

PDB gallery

1p1t: NMR Structure of the N-terminal RRM domain of Cleavage stimulation factor 64 KDa subunit

2j8p: NMR STRUCTURE OF C-TERMINAL DOMAIN OF HUMAN CSTF-64

CSTF2

Interactions

References

Further reading