CRSP3

Mediator complex subunit 23

Identifiers

MED23; ARC130; CRSP130; CRSP133; CRSP3; DKFZp434H0117; DRIP130; SUR-2; SUR2

External IDs

OMIM: 605042 MGI: 1917458 HomoloGene: 3552 GeneCards: MED23 Gene

Gene Ontology
Molecular function	• transcription coactivator activity • protein binding
Cellular component	• nucleus • nucleoplasm • transcription factor complex
Biological process	• regulation of transcription, DNA-dependent • regulation of transcription from RNA polymerase II promoter • transcription initiation from RNA polymerase II promoter • gene expression
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

n/a

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 6:
131.9 – 131.95 Mb

Chr 10:
24.59 – 24.63 Mb

PubMed search

[1]

[2]

Mediator of RNA polymerase II transcription subunit 23 is an enzyme that in humans is encoded by the MED23 gene.^[1]^[2]

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a metastasis suppressor. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.^[2]

Interactions

CRSP3 has been shown to interact with Estrogen receptor alpha,^[3] CEBPB^[4] and Cyclin-dependent kinase 8.^[5]^[3]

References

^ Ryu S, Zhou S, Ladurner AG, Tjian R (Feb 1999). "The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1". Nature 397 (6718): 446–50. doi:10.1038/17141. PMID 9989412.
^ ^a ^b "Entrez Gene: CRSP3 cofactor required for Sp1 transcriptional activation, subunit 3, 130kDa". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=9439.
^ ^a ^b Kang, Yun Kyoung; Guermah Mohamed, Yuan Chao-Xing, Roeder Robert G (Mar. 2002). "The TRAP/Mediator coactivator complex interacts directly with estrogen receptors alpha and beta through the TRAP220 subunit and directly enhances estrogen receptor function in vitro". Proc. Natl. Acad. Sci. U.S.A. (United States) 99 (5): 2642–7. doi:10.1073/pnas.261715899. ISSN 0027-8424. PMC 122401. PMID 11867769. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=122401.
^ Mo, Xianming; Kowenz-Leutz Elisabeth, Xu Hong, Leutz Achim (Jan. 2004). "Ras induces mediator complex exchange on C/EBP beta". Mol. Cell (United States) 13 (2): 241–50. doi:10.1016/S1097-2765(03)00521-5. ISSN 1097-2765. PMID 14759369.
^ Wang, G; Cantin G T, Stevens J L, Berk A J (Jul. 2001). "Characterization of mediator complexes from HeLa cell nuclear extract". Mol. Cell. Biol. (United States) 21 (14): 4604–13. doi:10.1128/MCB.21.14.4604-4613.2001. ISSN 0270-7306. PMC 87123. PMID 11416138. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=87123.

Rachez C, Lemon BD, Suldan Z, et al. (1999). "Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex.". Nature 398 (6730): 824–8. doi:10.1038/19783. PMID 10235266.
Näär AM, Beaurang PA, Zhou S, et al. (1999). "Composite co-activator ARC mediates chromatin-directed transcriptional activation.". Nature 398 (6730): 828–32. doi:10.1038/19789. PMID 10235267.
Boyer TG, Martin ME, Lees E, et al. (1999). "Mammalian Srb/Mediator complex is targeted by adenovirus E1A protein.". Nature 399 (6733): 276–9. doi:10.1038/20466. PMID 10353252.
Nagase T, Ishikawa K, Kikuno R, et al. (2000). "Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.". DNA Res. 6 (5): 337–45. doi:10.1093/dnares/6.5.337. PMID 10574462.
Ko L, Cardona GR, Chin WW (2000). "Thyroid hormone receptor-binding protein, an LXXLL motif-containing protein, functions as a general coactivator.". Proc. Natl. Acad. Sci. U.S.A. 97 (11): 6212–7. doi:10.1073/pnas.97.11.6212. PMC 18584. PMID 10823961. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=18584.
Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination.". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=310948.
Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs.". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=311072.
Stevens JL, Cantin GT, Wang G, et al. (2002). "Transcription control by E1A and MAP kinase pathway via Sur2 mediator subunit.". Science 296 (5568): 755–8. doi:10.1126/science.1068943. PMID 11934987.
Asada S, Choi Y, Yamada M, et al. (2002). "External control of Her2 expression and cancer cell growth by targeting a Ras-linked coactivator.". Proc. Natl. Acad. Sci. U.S.A. 99 (20): 12747–52. doi:10.1073/pnas.202162199. PMC 130531. PMID 12242338. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=130531.
Nakayama M, Kikuno R, Ohara O (2003). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs.". Genome Res. 12 (11): 1773–84. doi:10.1101/gr.406902. PMC 187542. PMID 12421765. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=187542.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Goldberg SF, Miele ME, Hatta N, et al. (2003). "Melanoma metastasis suppression by chromosome 6: evidence for a pathway regulated by CRSP3 and TXNIP.". Cancer Res. 63 (2): 432–40. PMID 12543799.
Mungall AJ, Palmer SA, Sims SK, et al. (2003). "The DNA sequence and analysis of human chromosome 6.". Nature 425 (6960): 805–11. doi:10.1038/nature02055. PMID 14574404.
Sato S, Tomomori-Sato C, Banks CA, et al. (2004). "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex.". J. Biol. Chem. 278 (50): 49671–4. doi:10.1074/jbc.C300444200. PMID 14576168.
Tomomori-Sato C, Sato S, Parmely TJ, et al. (2004). "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2.". J. Biol. Chem. 279 (7): 5846–51. doi:10.1074/jbc.M312523200. PMID 14638676.
Mo X, Kowenz-Leutz E, Xu H, Leutz A (2004). "Ras induces mediator complex exchange on C/EBP beta.". Mol. Cell 13 (2): 241–50. doi:10.1016/S1097-2765(03)00521-5. PMID 14759369.
Sato S, Tomomori-Sato C, Parmely TJ, et al. (2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology.". Mol. Cell 14 (5): 685–91. doi:10.1016/j.molcel.2004.05.006. PMID 15175163.
Wada O, Oishi H, Takada I, et al. (2004). "BRCA1 function mediates a TRAP/DRIP complex through direct interaction with TRAP220.". Oncogene 23 (35): 6000–5. doi:10.1038/sj.onc.1207786. PMID 15208681.
Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to biology: a functional genomics pipeline.". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528930.

CRSP3

Interactions

References

Further reading