CHD8

Chromodomain helicase DNA binding protein 8

Solution structure of the first BRK domain from human CHD8. PDB rendering based on 2dl6.

Available structures
PDB	2CKA, 2DL6

Identifiers

CHD8; DKFZp686N17164; HELSNF1; KIAA1564

External IDs

OMIM: 610528 MGI: 1915022 HomoloGene: 72405 GeneCards: CHD8 Gene

Gene Ontology
Molecular function	• nucleotide binding • p53 binding • DNA binding • DNA helicase activity • protein binding • ATP binding • beta-catenin binding • DNA-dependent ATPase activity • hydrolase activity, acting on acid anhydrides • methylated histone residue binding • histone binding
Cellular component	• nucleus • protein complex • MLL1 complex
Biological process	• chromatin modification • negative regulation of Wnt receptor signaling pathway • ATP-dependent chromatin remodeling • negative regulation of transcription, DNA-dependent • positive regulation of transcription, DNA-dependent • positive regulation of transcription from RNA polymerase II promoter • positive regulation of transcription from RNA polymerase III promoter • canonical Wnt receptor signaling pathway
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

ENSG00000100888

ENSMUSG00000053754

n/a

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 14:
21.85 – 21.91 Mb

Chr 14:
52.82 – 52.86 Mb

PubMed search

Chromodomain-helicase-DNA-binding protein 8 is an enzyme that in humans is encoded by the CHD8 gene.^[1]^[2]

References

^ Nagase T, Kikuno R, Nakayama M, Hirosawa M, Ohara O (Dec 2000). "Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res 7 (4): 273–81. doi:10.1093/dnares/7.4.271. PMID 10997877.
^ "Entrez Gene: CHD8 chromodomain helicase DNA binding protein 8". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=57680.

Further reading

Nakajima D, Okazaki N, Yamakawa H et al. (2003). "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones". DNA Res. 9 (3): 99–106. doi:10.1093/dnares/9.3.99. PMID 12168954.
Epplen C, Epplen JT (1994). "Expression of (cac)n/(gtg)n simple repetitive sequences in mRNA of human lymphocytes". Hum. Genet. 93 (1): 35–41. doi:10.1007/BF00218910. PMID 7505766.
Sakamoto I, Kishida S, Fukui A et al. (2000). "A novel beta-catenin-binding protein inhibits beta-catenin-dependent Tcf activation and axis formation". J. Biol. Chem. 275 (42): 32871–8. doi:10.1074/jbc.M004089200. PMID 10921920.
Kobayashi M, Hanai R (2001). "M phase-specific association of human topoisomerase IIIbeta with chromosomes". Biochem. Biophys. Res. Commun. 287 (1): 282–7. doi:10.1006/bbrc.2001.5580. PMID 11549288.
Kobayashi M, Kishida S, Fukui A et al. (2002). "Nuclear localization of Duplin, a beta-catenin-binding protein, is essential for its inhibitory activity on the Wnt signaling pathway". J. Biol. Chem. 277 (8): 5816–22. doi:10.1074/jbc.M108433200. PMID 11744694.
Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Ota T, Suzuki Y, Nishikawa T et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Nishiyama M, Nakayama K, Tsunematsu R et al. (2004). "Early Embryonic Death in Mice Lacking the β-Catenin-Binding Protein Duplin". Mol. Cell. Biol. 24 (19): 8386–94. doi:10.1128/MCB.24.19.8386-8394.2004. PMC 516734. PMID 15367660. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=516734.
Lin KT, Lu RM, Tarn WY (2004). "The WW Domain-Containing Proteins Interact with the Early Spliceosome and Participate in Pre-mRNA Splicing In Vivo". Mol. Cell. Biol. 24 (20): 9176–85. doi:10.1128/MCB.24.20.9176-9185.2004. PMC 517884. PMID 15456888. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=517884.
Gerhard DS, Wagner L, Feingold EA et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Ishihara K, Oshimura M, Nakao M (2006). "CTCF-dependent chromatin insulator is linked to epigenetic remodeling". Mol. Cell 23 (5): 733–42. doi:10.1016/j.molcel.2006.08.008. PMID 16949368.
Beausoleil SA, Villén J, Gerber SA et al. (2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nat. Biotechnol. 24 (10): 1285–92. doi:10.1038/nbt1240. PMID 16964243.
Olsen JV, Blagoev B, Gnad F et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.
Yuan, CC, Zhao, X et al. (2007). "CHD8 Associates with Human Staf and Contributes to Efficient U6 RNA Polymerase III Transcription". Mol Cell Biol 27 (24): 8729–38. doi:10.1128/MCB.00846-07. PMC 2169411. PMID 17938208. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2169411.
Thompson, BA, Tremblay, V et al. (2008). "CHD8 Is an ATP-Dependent Chromatin Remodeling Factor That Regulates β-Catenin Target Genes". Mol Cell Biol 28 (12): 3894–904. doi:10.1128/MCB.00322-08. PMC 2423111. PMID 18378692. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2423111.
Caldon, CE, Sergio, CM et al. (2009). "Estrogen Regulation of Cyclin E2 Requires Cyclin D1 but Not c-Myc". Mol Cell Biol 29 (17): 4623–39. doi:10.1128/MCB.00269-09. PMC 2725719. PMID 19564413. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2725719.
Nishiyama, M, Oshikawa, K et al. (2009). "CHD8 suppresses p53-mediated apoptosis through histone H1 recruitment during early embryogenesis". Nat Cell Biol 11 (2): 172–82. doi:10.1038/ncb1831. PMC 3132516. PMID 19151705. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=3132516.
Rodriguez-Paredes, M, Ceballos-Chavez, M et al. (2009). "The chromatin remodeling factor CHD8 interacts with elongating RNA polymerase II and controls expression of the cyclin E2 gene". Nucleic Acids Res 37 (8): 2449–60. doi:10.1093/nar/gkp101. PMC 2677868. PMID 19255092. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2677868.
Yates, JA, Menon, T et al. (2010). "Regulation of HOXA2 gene expression by the ATP-dependent chromatin remodeling enzyme CHD8". FEBS Lett 584 (4): 689–93. doi:10.1016/j.febslet.2010.01.022. PMID 20085832.

PDB gallery

2dl6: Solution structure of the first BRK domain from human chromodomain-helicase-DNA-binding protein 8