CHAF1A

Chromatin assembly factor 1, subunit A (p150)

Identifiers

CHAF1A; CAF-1; CAF1; CAF1B; CAF1P150; MGC71229; P150

External IDs

OMIM: 601246 MGI: 1351331 HomoloGene: 4003 GeneCards: CHAF1A Gene

Gene Ontology
Molecular function	• chromatin binding • protein binding • unfolded protein binding • chromo shadow domain binding
Cellular component	• nucleus • chromatin assembly complex • CAF-1 complex • WINAC complex
Biological process	• DNA replication • DNA repair • DNA replication-dependent nucleosome assembly • regulation of transcription, DNA-dependent • protein complex assembly • cell cycle • chromatin assembly
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 19:
4.4 – 4.44 Mb

Chr 17:
56.18 – 56.21 Mb

PubMed search

[1]

[2]

Chromatin assembly factor 1 subunit A is a protein that in humans is encoded by the CHAF1A gene.^[1]^[2]

Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995).[supplied by OMIM]^[2]

Interactions

CHAF1A has been shown to interact with ASF1B,^[3]^[4] ASF1A,^[4] MBD1,^[5] Bloom syndrome protein^[6] and CBX5.^[5]^[7]

References

^ Kaufman PD, Kobayashi R, Kessler N, Stillman B (Aug 1995). "The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA replication". Cell 81 (7): 1105–14. doi:10.1016/S0092-8674(05)80015-7. PMID 7600578.
^ ^a ^b "Entrez Gene: CHAF1A chromatin assembly factor 1, subunit A (p150)". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=10036.
^ Ewing, Rob M; Chu Peter, Elisma Fred, Li Hongyan, Taylor Paul, Climie Shane, McBroom-Cerajewski Linda, Robinson Mark D, O'Connor Liam, Li Michael, Taylor Rod, Dharsee Moyez, Ho Yuen, Heilbut Adrian, Moore Lynda, Zhang Shudong, Ornatsky Olga, Bukhman Yury V, Ethier Martin, Sheng Yinglun, Vasilescu Julian, Abu-Farha Mohamed, Lambert Jean-Philippe, Duewel Henry S, Stewart Ian I, Kuehl Bonnie, Hogue Kelly, Colwill Karen, Gladwish Katharine, Muskat Brenda, Kinach Robert, Adams Sally-Lin, Moran Michael F, Morin Gregg B, Topaloglou Thodoros, Figeys Daniel (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. (England) 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1847948.
^ ^a ^b Mello, Jill A; Silljé Herman H W, Roche Daniele M J, Kirschner Doris B, Nigg Erich A, Almouzni Geneviève (Apr. 2002). "Human Asf1 and CAF-1 interact and synergize in a repair-coupled nucleosome assembly pathway". EMBO Rep. (England) 3 (4): 329–34. doi:10.1093/embo-reports/kvf068. ISSN 1469-221X. PMC 1084056. PMID 11897662. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1084056.
^ ^a ^b Reese, Brian E; Bachman Kurtis E, Baylin Stephen B, Rountree Michael R (May. 2003). "The Methyl-CpG Binding Protein MBD1 Interacts with the p150 Subunit of Chromatin Assembly Factor 1". Mol. Cell. Biol. (United States) 23 (9): 3226–36. doi:10.1128/MCB.23.9.3226-3236.2003. ISSN 0270-7306. PMC 153189. PMID 12697822. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=153189.
^ Jiao, Renjie; Bachrati Csanád Z, Pedrazzi Graziella, Kuster Patrick, Petkovic Maja, Li Ji-Liang, Egli Dieter, Hickson Ian D, Stagljar Igor (Jun. 2004). "Physical and Functional Interaction between the Bloom's Syndrome Gene Product and the Largest Subunit of Chromatin Assembly Factor 1". Mol. Cell. Biol. (United States) 24 (11): 4710–9. doi:10.1128/MCB.24.11.4710-4719.2004. ISSN 0270-7306. PMC 416397. PMID 15143166. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=416397.
^ Lechner, M S; Begg G E, Speicher D W, Rauscher F J (Sep. 2000). "Molecular Determinants for Targeting Heterochromatin Protein 1-Mediated Gene Silencing: Direct Chromoshadow Domain–KAP-1 Corepressor Interaction Is Essential". Mol. Cell. Biol. (UNITED STATES) 20 (17): 6449–65. doi:10.1128/MCB.20.17.6449-6465.2000. ISSN 0270-7306. PMC 86120. PMID 10938122. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=86120.

Ridgway P, Almouzni G (2000). "CAF-1 and the inheritance of chromatin states: at the crossroads of DNA replication and repair". J. Cell. Sci. 113 ( Pt 15): 2647–58. PMID 10893180.
Verreault A, Kaufman PD, Kobayashi R, Stillman B (1996). "Nucleosome assembly by a complex of CAF-1 and acetylated histones H3/H4". Cell 87 (1): 95–104. doi:10.1016/S0092-8674(00)81326-4. PMID 8858152.
Marheineke K, Krude T (1998). "Nucleosome assembly activity and intracellular localization of human CAF-1 changes during the cell division cycle". J. Biol. Chem. 273 (24): 15279–86. doi:10.1074/jbc.273.24.15279. PMID 9614144.
Shibahara K, Stillman B (1999). "Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin". Cell 96 (4): 575–85. doi:10.1016/S0092-8674(00)80661-3. PMID 10052459.
Murzina N, Verreault A, Laue E, Stillman B (1999). "Heterochromatin dynamics in mouse cells: interaction between chromatin assembly factor 1 and HP1 proteins". Mol. Cell 4 (4): 529–40. doi:10.1016/S1097-2765(00)80204-X. PMID 10549285.
Moggs JG, Grandi P, Quivy JP, et al. (2000). "A CAF-1–PCNA-Mediated Chromatin Assembly Pathway Triggered by Sensing DNA Damage". Mol. Cell. Biol. 20 (4): 1206–18. doi:10.1128/MCB.20.4.1206-1218.2000. PMC 85246. PMID 10648606. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=85246.
Lechner MS, Begg GE, Speicher DW, Rauscher FJ (2000). "Molecular Determinants for Targeting Heterochromatin Protein 1-Mediated Gene Silencing: Direct Chromoshadow Domain–KAP-1 Corepressor Interaction Is Essential". Mol. Cell. Biol. 20 (17): 6449–65. doi:10.1128/MCB.20.17.6449-6465.2000. PMC 86120. PMID 10938122. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=86120.
Dong H, Lin W, Zhang CK, et al. (2001). "Genomic sequence and expression analyses of human chromatin assembly factor 1 p150 gene". Gene 264 (2): 187–96. doi:10.1016/S0378-1119(01)00335-3. PMID 11250073.
Mello JA, Silljé HH, Roche DM, et al. (2002). "Human Asf1 and CAF-1 interact and synergize in a repair-coupled nucleosome assembly pathway". EMBO Rep. 3 (4): 329–34. doi:10.1093/embo-reports/kvf068. PMC 1084056. PMID 11897662. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1084056.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Reese BE, Bachman KE, Baylin SB, Rountree MR (2003). "The Methyl-CpG Binding Protein MBD1 Interacts with the p150 Subunit of Chromatin Assembly Factor 1". Mol. Cell. Biol. 23 (9): 3226–36. doi:10.1128/MCB.23.9.3226-3236.2003. PMC 153189. PMID 12697822. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=153189.
Morel AP, Sentis S, Bianchin C, et al. (2004). "BTG2 antiproliferative protein interacts with the human CCR4 complex existing in vivo in three cell-cycle-regulated forms". J. Cell. Sci. 116 (Pt 14): 2929–36. doi:10.1242/jcs.00480. PMID 12771185.
Kitagawa H, Fujiki R, Yoshimura K, et al. (2003). "The chromatin-remodeling complex WINAC targets a nuclear receptor to promoters and is impaired in Williams syndrome". Cell 113 (7): 905–17. doi:10.1016/S0092-8674(03)00436-7. PMID 12837248.
Glinsky GV, Glinskii AB, Stephenson AJ, et al. (2004). "Gene expression profiling predicts clinical outcome of prostate cancer". J. Clin. Invest. 113 (6): 913–23. doi:10.1172/JCI20032. PMC 362118. PMID 15067324. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=362118.
Jiao R, Bachrati CZ, Pedrazzi G, et al. (2004). "Physical and Functional Interaction between the Bloom's Syndrome Gene Product and the Largest Subunit of Chromatin Assembly Factor 1". Mol. Cell. Biol. 24 (11): 4710–9. doi:10.1128/MCB.24.11.4710-4719.2004. PMC 416397. PMID 15143166. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=416397.
Lehner B, Sanderson CM (2004). "A Protein Interaction Framework for Human mRNA Degradation". Genome Res. 14 (7): 1315–23. doi:10.1101/gr.2122004. PMC 442147. PMID 15231747. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=442147.
Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=514446.
Sarraf SA, Stancheva I (2004). "Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly". Mol. Cell 15 (4): 595–605. doi:10.1016/j.molcel.2004.06.043. PMID 15327775.

CHAF1A

Interactions

References

Further reading