CENTG1

ArfGAP with GTPase domain, ankyrin repeat and PH domain 2

PDB rendering based on 2bmj.

Available structures
PDB	2BMJ, 2IWR, 2RLO

Identifiers

Symbols

AGAP2; CENTG1; FLJ16430; GGAP2; KIAA0167; PIKE

External IDs

OMIM: 605476 HomoloGene: 86815 GeneCards: AGAP2 Gene

Gene Ontology
Molecular function	• nucleotide binding • protein binding • GTP binding • ARF GTPase activator activity • zinc ion binding • protein kinase binding • protein kinase activator activity • metal ion binding
Cellular component	• intracellular • nucleus • nucleus • nucleolus • cytoplasm • mitochondrion
Biological process	• small GTPase mediated signal transduction • axon guidance • protein transport • regulation of ARF GTPase activity • positive regulation of mammary gland epithelial cell proliferation • negative regulation of protein catabolic process • negative regulation of neuron apoptosis • positive regulation of transcription from RNA polymerase II promoter • positive regulation of JAK-STAT cascade • mammary gland alveolus development
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

n/a

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 12:
58.12 – 58.14 Mb

Chr 10:
126.52 – 126.53 Mb

PubMed search

[1]

[2]

Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 is an enzyme that in humans is encoded by the AGAP2 gene.^[1]

Interactions

CENTG1 has been shown to interact with PIK3R1,^[2] HOMER1^[3] and EPB41L1.^[2]

References

^ "Entrez Gene: CENTG1 centaurin, gamma 1". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=116986.
^ ^a ^b Ye, K; Hurt K J, Wu F Y, Fang M, Luo H R, Hong J J, Blackshaw S, Ferris C D, Snyder S H (Dec. 2000). "Pike. A nuclear gtpase that enhances PI3kinase activity and is regulated by protein 4.1N". Cell (UNITED STATES) 103 (6): 919–30. doi:10.1016/S0092-8674(00)00195-1. ISSN 0092-8674. PMID 11136977.
^ Rong, Rong; Ahn Jee-Yin, Huang Honglian, Nagata Eiichiro, Kalman Daniel, Kapp Judith A, Tu Jiancheng, Worley Paul F, Snyder Solomon H, Ye Keqiang (Nov. 2003). "PI3 kinase enhancer-Homer complex couples mGluRI to PI3 kinase, preventing neuronal apoptosis". Nat. Neurosci. (United States) 6 (11): 1153–61. doi:10.1038/nn1134. ISSN 1097-6256. PMID 14528310.

Nagase T, Seki N, Ishikawa K, et al. (1996). "Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1.". DNA Res. 3 (1): 17–24. doi:10.1093/dnares/3.1.17. PMID 8724849.
Elkahloun AG, Krizman DB, Wang Z, et al. (1997). "Transcript mapping in a 46-kb sequenced region at the core of 12q13.3 amplification in human cancers.". Genomics 42 (2): 295–301. doi:10.1006/geno.1997.4727. PMID 9192850.
Ye K, Hurt KJ, Wu FY, et al. (2001). "Pike. A nuclear gtpase that enhances PI3kinase activity and is regulated by protein 4.1N.". Cell 103 (6): 919–30. doi:10.1016/S0092-8674(00)00195-1. PMID 11136977.
Ye K, Aghdasi B, Luo HR, et al. (2002). "Phospholipase C gamma 1 is a physiological guanine nucleotide exchange factor for the nuclear GTPase PIKE.". Nature 415 (6871): 541–4. doi:10.1038/415541a. PMID 11823862.
Nie Z, Stanley KT, Stauffer S, et al. (2003). "AGAP1, an endosome-associated, phosphoinositide-dependent ADP-ribosylation factor GTPase-activating protein that affects actin cytoskeleton.". J. Biol. Chem. 277 (50): 48965–75. doi:10.1074/jbc.M202969200. PMID 12388557.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Xia C, Ma W, Stafford LJ, et al. (2003). "GGAPs, a new family of bifunctional GTP-binding and GTPase-activating proteins.". Mol. Cell. Biol. 23 (7): 2476–88. doi:10.1128/MCB.23.7.2476-2488.2003. PMC 150724. PMID 12640130. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=150724.
Rong R, Ahn JY, Huang H, et al. (2003). "PI3 kinase enhancer-Homer complex couples mGluRI to PI3 kinase, preventing neuronal apoptosis.". Nat. Neurosci. 6 (11): 1153–61. doi:10.1038/nn1134. PMID 14528310.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Ahn JY, Rong R, Kroll TG, et al. (2004). "PIKE (phosphatidylinositol 3-kinase enhancer)-A GTPase stimulates Akt activity and mediates cellular invasion.". J. Biol. Chem. 279 (16): 16441–51. doi:10.1074/jbc.M312175200. PMID 14761976.
Ahn JY, Hu Y, Kroll TG, et al. (2004). "PIKE-A is amplified in human cancers and prevents apoptosis by up-regulating Akt.". Proc. Natl. Acad. Sci. U.S.A. 101 (18): 6993–8. doi:10.1073/pnas.0400921101. PMC 406454. PMID 15118108. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=406454.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Nie Z, Fei J, Premont RT, Randazzo PA (2005). "The Arf GAPs AGAP1 and AGAP2 distinguish between the adaptor protein complexes AP-1 and AP-3.". J. Cell. Sci. 118 (Pt 15): 3555–66. doi:10.1242/jcs.02486. PMID 16079295.
Knobbe CB, Trampe-Kieslich A, Reifenberger G (2005). "Genetic alteration and expression of the phosphoinositol-3-kinase/Akt pathway genes PIK3CA and PIKE in human glioblastomas.". Neuropathol. Appl. Neurobiol. 31 (5): 486–90. doi:10.1111/j.1365-2990.2005.00660.x. PMID 16150119.
Hu Y, Liu Z, Ye K (2006). "Phosphoinositol lipids bind to phosphatidylinositol 3 (PI3)-kinase enhancer GTPase and mediate its stimulatory effect on PI3-kinase and Akt signalings.". Proc. Natl. Acad. Sci. U.S.A. 102 (46): 16853–8. doi:10.1073/pnas.0507365102. PMC 1283830. PMID 16263930. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1283830.
Tang X, Feng Y, Ye K (2007). "Src-family tyrosine kinase fyn phosphorylates phosphatidylinositol 3-kinase enhancer-activating Akt, preventing its apoptotic cleavage and promoting cell survival.". Cell Death Differ. 14 (2): 368–77. doi:10.1038/sj.cdd.4402011. PMID 16841086.
Soundararajan M, Yang X, Elkins JM, et al. (2007). "The centaurin gamma-1 GTPase-like domain functions as an NTPase.". Biochem. J. 401 (3): 679–88. doi:10.1042/BJ20060555. PMC 1770848. PMID 17037982. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1770848.
Werden SJ, Barrett JW, Wang G, et al. (2007). "M-T5, the ankyrin repeat, host range protein of myxoma virus, activates Akt and can be functionally replaced by cellular PIKE-A.". J. Virol. 81 (5): 2340–8. doi:10.1128/JVI.01310-06. PMC 1865929. PMID 17151107. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1865929.
Liu X, Hu Y, Hao C, et al. (2007). "PIKE-A is a proto-oncogene promoting cell growth, transformation and invasion.". Oncogene 26 (34): 4918–27. doi:10.1038/sj.onc.1210290. PMID 17297440.
Dunphy JL, Ye K, Casanova JE (2007). "Nuclear functions of the Arf guanine nucleotide exchange factor BRAG2.". Traffic 8 (6): 661–72. doi:10.1111/j.1600-0854.2007.00561.x. PMID 17461797.

PDB gallery

2bmj: GTPASE LIKE DOMAIN OF CENTAURIN GAMMA 1 (HUMAN)

2iwr: GTPASE LIKE DOMAIN OF CENTAURIN GAMMA 1 (HUMAN)

CENTG1

Interactions

References

Further reading