CDC16

Cell division cycle 16 homolog (S. cerevisiae)

Identifiers

CDC16; ANAPC6; APC6; CUT9

External IDs

OMIM: 603461 MGI: 1917207 HomoloGene: 2899 GeneCards: CDC16 Gene

Gene Ontology
Molecular function	• binding
Cellular component	• nucleus • nucleoplasm • nucleoplasm • anaphase-promoting complex • cytoplasm • centrosome • spindle • cytosol • cytosol • cytoskeleton • spindle microtubule
Biological process	• cell cycle checkpoint • mitotic cell cycle • mitosis • regulation of mitosis • mitotic cell cycle spindle assembly checkpoint • cell proliferation • anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process • anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process • cell division • negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle • positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle • regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle • protein K11-linked ubiquitination
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 13:
115 – 115.04 Mb

Chr 8:
13.76 – 13.78 Mb

PubMed search

[1]

[2]

Cell division cycle protein 16 homolog is a protein that in humans is encoded by the CDC16 gene.^[1]^[2]

This gene encodes a component protein of the APC complex, which is composed of eight proteins and functions as a protein ubiquitin ligase. The APC complex is a cyclin degradation system that governs exit from mitosis. Each component protein of the APC complex is highly conserved among eukaryotic organisms. This protein and two other APC complex proteins, CDC23 and CDC27, contain a tetratricopeptide repeat (TPR), a protein domain that may be involved in protein-protein interaction. Multiple alternatively spliced variants, encoding the same protein, have been identified.^[2]

Interactions

CDC16 has been shown to interact with CDC27^[3]^[4]^[5]^[6] and CDC20.^[3]^[5]^[7]^[8]

References

^ Tugendreich S, Tomkiel J, Earnshaw W, Hieter P (Jun 1995). "CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition". Cell 81 (2): 261–8. doi:10.1016/0092-8674(95)90336-4. PMID 7736578.
^ ^a ^b "Entrez Gene: CDC16 cell division cycle 16 homolog (S. cerevisiae)". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=8881.
^ ^a ^b Vodermaier, Hartmut C; Gieffers Christian, Maurer-Stroh Sebastian, Eisenhaber Frank, Peters Jan-Michael (Sep. 2003). "TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Curr. Biol. (England) 13 (17): 1459–68. doi:10.1016/S0960-9822(03)00581-5. ISSN 0960-9822. PMID 12956947.
^ Ollendorff, V; Donoghue D J (Dec. 1997). "The serine/threonine phosphatase PP5 interacts with CDC16 and CDC27, two tetratricopeptide repeat-containing subunits of the anaphase-promoting complex". J. Biol. Chem. (UNITED STATES) 272 (51): 32011–8. doi:10.1074/jbc.272.51.32011. ISSN 0021-9258. PMID 9405394.
^ ^a ^b Kallio, M; Weinstein J, Daum J R, Burke D J, Gorbsky G J (Jun. 1998). "Mammalian p55CDC Mediates Association of the Spindle Checkpoint Protein Mad2 with the Cyclosome/Anaphase-promoting Complex, and is Involved in Regulating Anaphase Onset and Late Mitotic Events". J. Cell Biol. (UNITED STATES) 141 (6): 1393–406. doi:10.1083/jcb.141.6.1393. ISSN 0021-9525. PMC 2132789. PMID 9628895. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2132789.
^ Gmachl, M; Gieffers C, Podtelejnikov A V, Mann M, Peters J M (Aug. 2000). "The RING-H2 finger protein APC11 and the E2 enzyme UBC4 are sufficient to ubiquitinate substrates of the anaphase-promoting complex". Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 97 (16): 8973–8. doi:10.1073/pnas.97.16.8973. ISSN 0027-8424. PMC 16806. PMID 10922056. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=16806.
^ Kallio, Marko J; Beardmore Victoria A, Weinstein Jasminder, Gorbsky Gary J (Sep. 2002). "Rapid microtubule-independent dynamics of Cdc20 at kinetochores and centrosomes in mammalian cells". J. Cell Biol. (United States) 158 (5): 841–7. doi:10.1083/jcb.200201135. ISSN 0021-9525. PMC 2173153. PMID 12196507. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2173153.
^ Nilsson, Jakob; Yekezare Mona, Minshull Jeremy, Pines Jonathon (Dec. 2008). "The APC/C maintains the spindle assembly checkpoint by targeting Cdc20 for destruction". Nat. Cell Biol. (England) 10 (12): 1411–20. doi:10.1038/ncb1799. PMC 2635557. PMID 18997788. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2635557.

Ollendorff V, Donoghue DJ (1998). "The serine/threonine phosphatase PP5 interacts with CDC16 and CDC27, two tetratricopeptide repeat-containing subunits of the anaphase-promoting complex". J. Biol. Chem. 272 (51): 32011–8. doi:10.1074/jbc.272.51.32011. PMID 9405394.
Yu H, Peters JM, King RW, et al. (1998). "Identification of a cullin homology region in a subunit of the anaphase-promoting complex". Science 279 (5354): 1219–22. doi:10.1126/science.279.5354.1219. PMID 9469815.
Kallio M, Weinstein J, Daum JR, et al. (1998). "Mammalian p55CDC Mediates Association of the Spindle Checkpoint Protein Mad2 with the Cyclosome/Anaphase-promoting Complex, and is Involved in Regulating Anaphase Onset and Late Mitotic Events". J. Cell Biol. 141 (6): 1393–406. doi:10.1083/jcb.141.6.1393. PMC 2132789. PMID 9628895. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2132789.
Grossberger R, Gieffers C, Zachariae W, et al. (1999). "Characterization of the DOC1/APC10 subunit of the yeast and the human anaphase-promoting complex". J. Biol. Chem. 274 (20): 14500–7. doi:10.1074/jbc.274.20.14500. PMID 10318877.
Chan GK, Jablonski SA, Sudakin V, et al. (1999). "Human Bubr1 Is a Mitotic Checkpoint Kinase That Monitors Cenp-E Functions at Kinetochores and Binds the Cyclosome/APC". J. Cell Biol. 146 (5): 941–54. doi:10.1083/jcb.146.5.941. PMC 2169490. PMID 10477750. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2169490.
Gieffers C, Peters BH, Kramer ER, et al. (1999). "Expression of the CDH1-associated form of the anaphase-promoting complex in postmitotic neurons". Proc. Natl. Acad. Sci. U.S.A. 96 (20): 11317–22. doi:10.1073/pnas.96.20.11317. PMC 18031. PMID 10500174. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=18031.
Gmachl M, Gieffers C, Podtelejnikov AV, et al. (2000). "The RING-H2 finger protein APC11 and the E2 enzyme UBC4 are sufficient to ubiquitinate substrates of the anaphase-promoting complex". Proc. Natl. Acad. Sci. U.S.A. 97 (16): 8973–8. doi:10.1073/pnas.97.16.8973. PMC 16806. PMID 10922056. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=16806.
Jörgensen PM, Gräslund S, Betz R, et al. (2001). "Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex". Gene 262 (1–2): 51–9. doi:10.1016/S0378-1119(00)00511-4. PMID 11179667.
Simpson JC, Wellenreuther R, Poustka A, et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1083732.
Stroschein SL, Bonni S, Wrana JL, Luo K (2001). "Smad3 recruits the anaphase-promoting complex for ubiquitination and degradation of SnoN". Genes Dev. 15 (21): 2822–36. doi:10.1101/gad.912901. PMC 312804. PMID 11691834. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=312804.
Yasui K, Arii S, Zhao C, et al. (2002). "TFDP1, CUL4A, and CDC16 identified as targets for amplification at 13q34 in hepatocellular carcinomas". Hepatology 35 (6): 1476–84. doi:10.1053/jhep.2002.33683. PMID 12029633.
Kallio MJ, Beardmore VA, Weinstein J, Gorbsky GJ (2002). "Rapid microtubule-independent dynamics of Cdc20 at kinetochores and centrosomes in mammalian cells". J. Cell Biol. 158 (5): 841–7. doi:10.1083/jcb.200201135. PMC 2173153. PMID 12196507. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2173153.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Wang Q, Moyret-Lalle C, Couzon F, et al. (2003). "Alterations of anaphase-promoting complex genes in human colon cancer cells". Oncogene 22 (10): 1486–90. doi:10.1038/sj.onc.1206224. PMID 12629511.
Vodermaier HC, Gieffers C, Maurer-Stroh S, et al. (2004). "TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Curr. Biol. 13 (17): 1459–68. doi:10.1016/S0960-9822(03)00581-5. PMID 12956947.
Kraft C, Herzog F, Gieffers C, et al. (2004). "Mitotic regulation of the human anaphase-promoting complex by phosphorylation". EMBO J. 22 (24): 6598–609. doi:10.1093/emboj/cdg627. PMC 291822. PMID 14657031. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=291822.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Nousiainen M, Silljé HH, Sauer G, et al. (2006). "Phosphoproteome analysis of the human mitotic spindle". Proc. Natl. Acad. Sci. U.S.A. 103 (14): 5391–6. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1459365.

CDC16

Interactions

References

Further reading