BTAF1

BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae)

Identifiers

BTAF1; KIAA0940; MGC138406; MOT1; TAF(II)170; TAF172; TAFII170

External IDs

OMIM: 605191 MGI: 2147538 HomoloGene: 31978 GeneCards: BTAF1 Gene

Gene Ontology
Molecular function	• nucleotide binding • DNA binding • sequence-specific DNA binding transcription factor activity • helicase activity • ATP binding • hydrolase activity, acting on acid anhydrides
Cellular component	• nucleus
Biological process	• transcription, DNA-dependent • negative regulation of transcription, DNA-dependent
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

n/a

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 10:
93.68 – 93.79 Mb

Chr 19:
37 – 37.09 Mb

PubMed search

[1]

[2]

TATA-binding protein-associated factor 172 is a protein that in humans is encoded by the BTAF1 gene.^[1]^[2]^[3]

Initiation of transcription by RNA polymerase II requires the assistance of TATA box-binding protein (TBP; MIM 600075) and TBP-associated factors, or TAFs (e.g., TAF2B; MIM 604912), in 2 distinct complexes, TFIID and B-TFIID. The TFIID complex is composed of TBP and more than 8 TAFs. However, the majority of TBP is present in the B-TFIID complex, which is composed of TBP and TAFII170, also called TAF172, and has DNA-dependent ATPase activity.[supplied by OMIM]^[3]

Interactions

BTAF1 has been shown to interact with TATA binding protein.^[4]^[2]

References

^ van der Knaap JA, Borst JW, van der Vliet PC, Gentz R, Timmers HT (Dec 1997). "Cloning of the cDNA for the TATA-binding protein-associated factorII170 subunit of transcription factor B-TFIID reveals homology to global transcription regulators in yeast and Drosophila". Proc Natl Acad Sci U S A 94 (22): 11827–32. doi:10.1073/pnas.94.22.11827. PMC 23617. PMID 9342322. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=23617.
^ ^a ^b Chicca JJ 2nd, Auble DT, Pugh BF (Mar 1998). "Cloning and Biochemical Characterization of TAF-172, a Human Homolog of Yeast Mot1". Mol Cell Biol 18 (3): 1701–10. PMC 108885. PMID 9488487. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=108885.
^ ^a ^b "Entrez Gene: BTAF1 BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae)". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=9044.
^ Scully, R; Anderson S F, Chao D M, Wei W, Ye L, Young R A, Livingston D M, Parvin J D (May. 1997). "BRCA1 is a component of the RNA polymerase II holoenzyme". Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 94 (11): 5605–10. doi:10.1073/pnas.94.11.5605. ISSN 0027-8424. PMC 20825. PMID 9159119. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=20825.

Zhou Q, Sharp PA (1995). "Novel mechanism and factor for regulation by HIV-1 Tat". EMBO J. 14 (2): 321–8. PMC 398086. PMID 7835343. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=398086.
Parada CA, Yoon JB, Roeder RG (1995). "A novel LBP-1-mediated restriction of HIV-1 transcription at the level of elongation in vitro". J. Biol. Chem. 270 (5): 2274–83. doi:10.1074/jbc.270.5.2274. PMID 7836461.
Ou SH, Garcia-Martínez LF, Paulssen EJ, Gaynor RB (1994). "Role of flanking E box motifs in human immunodeficiency virus type 1 TATA element function". J. Virol. 68 (11): 7188–99. PMC 237158. PMID 7933101. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=237158.
Kashanchi F, Piras G, Radonovich MF, et al. (1994). "Direct interaction of human TFIID with the HIV-1 transactivator tat". Nature 367 (6460): 295–9. doi:10.1038/367295a0. PMID 8121496.
Wang Z, Morris GF, Rice AP, et al. (1996). "Wild-type and transactivation-defective mutants of human immunodeficiency virus type 1 Tat protein bind human TATA-binding protein in vitro". J. Acquir. Immune Defic. Syndr. Hum. Retrovirol. 12 (2): 128–38. doi:10.1097/00042560-199606010-00005. PMID 8680883.
Pendergrast PS, Morrison D, Tansey WP, Hernandez N (1996). "Mutations in the carboxy-terminal domain of TBP affect the synthesis of human immunodeficiency virus type 1 full-length and short transcripts similarly". J. Virol. 70 (8): 5025–34. PMC 190456. PMID 8764009. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=190456.
Kashanchi F, Khleif SN, Duvall JF, et al. (1996). "Interaction of human immunodeficiency virus type 1 Tat with a unique site of TFIID inhibits negative cofactor Dr1 and stabilizes the TFIID-TFIIA complex". J. Virol. 70 (8): 5503–10. PMC 190508. PMID 8764062. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=190508.
Zhou Q, Sharp PA (1996). "Tat-SF1: cofactor for stimulation of transcriptional elongation by HIV-1 Tat". Science 274 (5287): 605–10. doi:10.1126/science.274.5287.605. PMID 8849451.
García-Martínez LF, Ivanov D, Gaynor RB (1997). "Association of Tat with purified HIV-1 and HIV-2 transcription preinitiation complexes". J. Biol. Chem. 272 (11): 6951–8. doi:10.1074/jbc.272.11.6951. PMID 9054383.
Scully R, Anderson SF, Chao DM, et al. (1997). "BRCA1 is a component of the RNA polymerase II holoenzyme". Proc. Natl. Acad. Sci. U.S.A. 94 (11): 5605–10. doi:10.1073/pnas.94.11.5605. PMC 20825. PMID 9159119. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=20825.
Van Der Knaap JA, Van Den Boom V, Kuipers J, et al. (2000). "The gene for human TATA-binding-protein-associated factor (TAFII) 170: structure, promoter and chromosomal localization". Biochem. J. 345 Pt 3 (Pt 3): 521–7. PMC 1220786. PMID 10642510. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1220786.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Wadhwa R, Yaguchi T, Hasan MK, et al. (2003). "Mortalin-MPD (mevalonate pyrophosphate decarboxylase) interactions and their role in control of cellular proliferation". Biochem. Biophys. Res. Commun. 302 (4): 735–42. doi:10.1016/S0006-291X(03)00226-2. PMID 12646231.
Pereira LA, Klejman MP, Ruhlmann C, et al. (2004). "Molecular architecture of the basal transcription factor B-TFIID". J. Biol. Chem. 279 (21): 21802–7. doi:10.1074/jbc.M313519200. PMID 14988402.
Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=514446.
Klejman MP, Pereira LA, van Zeeburg HJ, et al. (2004). "NC2α Interacts with BTAF1 and Stimulates Its ATP-Dependent Association with TATA-Binding Protein". Mol. Cell. Biol. 24 (22): 10072–82. doi:10.1128/MCB.24.22.10072-10082.2004. PMC 525489. PMID 15509807. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=525489.
Grob P, Cruse MJ, Inouye C, et al. (2006). "Cryo-electron microscopy studies of human TFIID: conformational breathing in the integration of gene regulatory cues". Structure 14 (3): 511–20. doi:10.1016/j.str.2005.11.020. PMID 16531235.

BTAF1

Interactions

References

Further reading