BNIP3

BCL2/adenovirus E1B 19kDa interacting protein 3

PDB rendering based on 2j5d.

Available structures
PDB	2J5D, 2KA1, 2KA2

Identifiers

Symbols

BNIP3; NIP3

External IDs

OMIM: 603293 MGI: 109326 HomoloGene: 2990 GeneCards: BNIP3 Gene

Gene Ontology
Molecular function	• protein binding • identical protein binding • protein homodimerization activity • protein heterodimerization activity • GTPase binding
Cellular component	• nucleus • nucleus • nuclear envelope • nucleoplasm • cytoplasm • mitochondrion • mitochondrion • membrane • integral to membrane • dendrite • integral to mitochondrial outer membrane • mitochondrial membrane • mitochondrial membrane
Biological process	• response to hypoxia • DNA fragmentation involved in apoptotic nuclear change • chromatin remodeling • apoptosis • apoptosis • anti-apoptosis • induction of apoptosis • cell death • cell death • negative regulation of survival gene product expression • negative regulation of mitochondrial fusion • positive regulation of protein complex disassembly • mitochondrial fragmentation involved in apoptosis • interspecies interaction between organisms • negative regulation of membrane potential • regulation of mitochondrial membrane permeability • brown fat cell differentiation • neuron apoptosis • defense response to virus • cellular response to mechanical stimulus • cellular response to cobalt ion • cellular response to hypoxia • reactive oxygen species metabolic process • positive regulation of mitochondrial fission • positive regulation of release of cytochrome c from mitochondria
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

n/a

n/a

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 10:
133.78 – 133.8 Mb

n/a

PubMed search

[1]

[2]

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 is a protein that in humans is encoded by the BNIP3 gene.^[1]

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. This gene contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein encoded by this gene is known to induce apoptosis, even in the presence of BCL2.^[2]

Interactions

BNIP3 has been shown to interact with CD47,^[3] BCL2-like 1^[4] and Bcl-2.^[1]^[4]

References

^ ^a ^b Boyd JM, Malstrom S, Subramanian T, Venkatesh LK, Schaeper U, Elangovan B, D'Sa-Eipper C, Chinnadurai G (Nov 1994). "Adenovirus E1B 19 kDa and Bcl-2 proteins interact with a common set of cellular proteins". Cell 79 (2): 341–51. doi:10.1016/0092-8674(94)90202-X. PMID 7954800.
^ "Entrez Gene: BNIP3 BCL2/adenovirus E1B 19kDa interacting protein 3". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=664.
^ Lamy, Laurence; Ticchioni Michel, Rouquette-Jazdanian Alexandre K, Samson Michel, Deckert Marcel, Greenberg Arnold H, Bernard Alain (Jun. 2003). "CD47 and the 19 kDa interacting protein-3 (BNIP3) in T cell apoptosis". J. Biol. Chem. (United States) 278 (26): 23915–21. doi:10.1074/jbc.M301869200. ISSN 0021-9258. PMID 12690108.
^ ^a ^b Ray, R; Chen G, Vande Velde C, Cizeau J, Park J H, Reed J C, Gietz R D, Greenberg A H (Jan. 2000). "BNIP3 heterodimerizes with Bcl-2/Bcl-X(L) and induces cell death independent of a Bcl-2 homology 3 (BH3) domain at both mitochondrial and nonmitochondrial sites". J. Biol. Chem. (UNITED STATES) 275 (2): 1439–48. doi:10.1074/jbc.275.2.1439. ISSN 0021-9258. PMID 10625696.

Kataoka N, Ohno M, Moda I, Shimura Y (1995). "Identification of the factors that interact with NCBP, an 80 kDa nuclear cap binding protein.". Nucleic Acids Res. 23 (18): 3638–41. doi:10.1093/nar/23.18.3638. PMC 307259. PMID 7478990. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=307259.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Suzuki Y; Yoshitomo-Nakagawa K; Maruyama K et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Chen G; Ray R; Dubik D et al. (1998). "The E1B 19K/Bcl-2-binding protein Nip3 is a dimeric mitochondrial protein that activates apoptosis". J. Exp. Med. 186 (12): 1975–83. doi:10.1084/jem.186.12.1975. PMC 2199165. PMID 9396766. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2199165.
Yasuda M, Theodorakis P, Subramanian T, Chinnadurai G (1998). "Adenovirus E1B-19K/BCL-2 interacting protein BNIP3 contains a BH3 domain and a mitochondrial targeting sequence". J. Biol. Chem. 273 (20): 12415–21. doi:10.1074/jbc.273.20.12415. PMID 9575197.
Chen G; Cizeau J; Vande Velde C et al. (1999). "Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins". J. Biol. Chem. 274 (1): 7–10. doi:10.1074/jbc.274.1.7. PMID 9867803.
Yasuda M; Han JW; Dionne CA et al. (1999). "BNIP3alpha: a human homolog of mitochondrial proapoptotic protein BNIP3". Cancer Res. 59 (3): 533–7. PMID 9973195.
Ohi N; Tokunaga A; Tsunoda H et al. (1999). "A novel adenovirus E1B19K-binding protein B5 inhibits apoptosis induced by Nip3 by forming a heterodimer through the C-terminal hydrophobic region". Cell Death Differ. 6 (4): 314–25. doi:10.1038/sj.cdd.4400493. PMID 10381623.
Ray R; Chen G; Vande Velde C et al. (2000). "BNIP3 heterodimerizes with Bcl-2/Bcl-X(L) and induces cell death independent of a Bcl-2 homology 3 (BH3) domain at both mitochondrial and nonmitochondrial sites". J. Biol. Chem. 275 (2): 1439–48. doi:10.1074/jbc.275.2.1439. PMID 10625696.
Vande Velde C; Cizeau J; Dubik D et al. (2000). "BNIP3 and genetic control of necrosis-like cell death through the mitochondrial permeability transition pore". Mol. Cell. Biol. 20 (15): 5454–68. doi:10.1128/MCB.20.15.5454-5468.2000. PMC 85997. PMID 10891486. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=85997.
Lee SM; Li ML; Tse YC et al. (2002). "Paeoniae Radix, a Chinese herbal extract, inhibit hepatoma cells growth by inducing apoptosis in a p53 independent pathway". Life Sci. 71 (19): 2267–77. doi:10.1016/S0024-3205(02)01962-8. PMID 12215374.
Strausberg RL; Feingold EA; Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Lamy L; Ticchioni M; Rouquette-Jazdanian AK et al. (2003). "CD47 and the 19 kDa interacting protein-3 (BNIP3) in T cell apoptosis". J. Biol. Chem. 278 (26): 23915–21. doi:10.1074/jbc.M301869200. PMID 12690108.
Kothari S; Cizeau J; McMillan-Ward E et al. (2003). "BNIP3 plays a role in hypoxic cell death in human epithelial cells that is inhibited by growth factors EGF and IGF". Oncogene 22 (30): 4734–44. doi:10.1038/sj.onc.1206666. PMID 12879018.
Deloukas P; Earthrowl ME; Grafham DV et al. (2004). "The DNA sequence and comparative analysis of human chromosome 10". Nature 429 (6990): 375–81. doi:10.1038/nature02462. PMID 15164054.
Okami J, Simeone DM, Logsdon CD (2004). "Silencing of the hypoxia-inducible cell death protein BNIP3 in pancreatic cancer". Cancer Res. 64 (15): 5338–46. doi:10.1158/0008-5472.CAN-04-0089. PMID 15289340.
Giatromanolaki A; Koukourakis MI; Sowter HM et al. (2005). "BNIP3 expression is linked with hypoxia-regulated protein expression and with poor prognosis in non-small cell lung cancer". Clin. Cancer Res. 10 (16): 5566–71. doi:10.1158/1078-0432.CCR-04-0076. PMID 15328198.
Gerhard DS; Wagner L; Feingold EA et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Shen XY, Zacal N, Singh G, Rainbow AJ (2005). "Alterations in mitochondrial and apoptosis-regulating gene expression in photodynamic therapy-resistant variants of HT29 colon carcinoma cells". Photochem. Photobiol. 81 (2): 306–13. doi:10.1562/2004-07-22-RA-242. PMID 15560738.

PDB gallery

2j5d: NMR STRUCTURE OF BNIP3 TRANSMEMBRANE DOMAIN IN LIPID BICELLES

BNIP3

Interactions

References

Further reading