| BCIP | |
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(5-bromo-4-chloro-1H-indol-3-yl) dihydrogen phosphate |
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Other names
BCIP |
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| Identifiers | |
| CAS number | 38404-93-2 |
| PubChem | 65409 |
| Jmol-3D images | Image 1 |
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| Properties | |
| Molecular formula | C8H6BrClNO4P |
| Molar mass | 326.47 g mol−1 |
| Appearance | Colorless |
| Solubility in water | Very low solubility in water or lipid |
| (verify) (what is: /?) Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) |
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| Infobox references | |
5-Bromo-4-chloro-3-indolyl phosphate (BCIP) is a chemical compound used in immunoblotting, in situ hybridization, and immunohistochemistry, with nitro blue tetrazolium chloride (NBT), for sensitive colorimetric detection of alkaline phosphatase.[1] BCIP is the alkaline phosphatase substrate, which is dephosphorylated by the enzyme and then dimerizes. This dimer reduces nitroblue tetrazolim (NBT) to form an insoluble dark blue diformazan precipitate. Alkaline phosphatase is commonly conjugated to secondary antibodies.