AZGP1

Alpha-2-glycoprotein 1, zinc-binding

PDB rendering based on 1t7v.

Available structures
PDB	1T7V, 1T7W, 1T7X, 1T7Y, 1T7Z, 1T80, 1ZAG, 3ES6

Identifiers

Symbols

AZGP1; ZA2G; ZAG

External IDs

OMIM: 194460 MGI: 103163 HomoloGene: 915 GeneCards: AZGP1 Gene

Gene Ontology
Molecular function	• ribonuclease activity • fatty acid binding • protein transmembrane transporter activity
Cellular component	• extracellular region • membrane • MHC class I protein complex
Biological process	• immune response • cell adhesion • cell adhesion • negative regulation of cell proliferation • lipid catabolic process • antigen processing and presentation
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 7:
99.56 – 99.57 Mb

Chr 5:
138.42 – 138.43 Mb

PubMed search

[1]

[2]

Zinc-alpha-2-glycoprotein is a protein that in humans is encoded by the AZGP1 gene.^[1]^[2]

This gene expresses a soluble protein that stimulates lipolysis, induces a reduction in body fat in mice, is associated with the cachexia related to cancer, and is known to be expressed in secretory cells of lung epithelium.^[3] In 2009, it was found that smoking increases expression of this gene, which is why smoking cessation leads to weight gain^[3].

References

^ Ueyama H, Niwa M, Tada T, Sasaki M, Ohkubo I (Jul 1991). "Cloning and nucleotide sequence of a human Zn-alpha 2-glycoprotein cDNA and chromosomal assignment of its gene". Biochem Biophys Res Commun 177 (2): 696–703. doi:10.1016/0006-291X(91)91844-3. PMID 2049092.
^ "Entrez Gene: AZGP1 alpha-2-glycoprotein 1, zinc-binding". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=563.
^ ^a ^b Vanni, H; Kazeros, A; Wang, R; Harvey, BG; Ferris, B; De, BP; Carolan, BJ; Hübner, RH et al. (2009). "Cigarette Smoking Induces Overexpression of a Fat-Depleting Gene AZGP1 in the Human.". Chest 135 (5): 1197–208. doi:10.1378/chest.08-1024. PMC 2679098. PMID 19188554. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2679098.

Freije JP, Fueyo A, Uría J, López-Otín C (1991). "Human Zn-alpha 2-glycoprotein cDNA cloning and expression analysis in benign and malignant breast tissues.". FEBS Lett. 290 (1–2): 247–9. doi:10.1016/0014-5793(91)81271-9. PMID 1915885.
Tada T, Ohkubo I, Niwa M, et al. (1991). "Immunohistochemical localization of Zn-alpha 2-glycoprotein in normal human tissues". J. Histochem. Cytochem. 39 (9): 1221–6. PMID 1918940.
Araki T, Gejyo F, Takagaki K, et al. (1988). "Complete amino acid sequence of human plasma Zn-alpha 2-glycoprotein and its homology to histocompatibility antigens". Proc. Natl. Acad. Sci. U.S.A. 85 (3): 679–83. doi:10.1073/pnas.85.3.679. PMC 279618. PMID 3422450. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=279618.
Shibata S, Miura K (1982). "Nephritogenic glycoprotein. IX. Plasma Zn-alpha2-glycoprotein as a second source of nephritogenic glycoprotein in urine". Nephron 31 (2): 170–6. PMID 6896906.
Ueyama H, Deng HX, Ohkubo I (1994). "Molecular cloning and chromosomal assignment of the gene for human Zn-alpha 2-glycoprotein". Biochemistry 32 (48): 12968–76. doi:10.1021/bi00211a004. PMID 8241150.
Freije JP, Fueyo A, Uría JA, et al. (1994). "Human Zn-alpha 2-glycoprotein: complete genomic sequence, identification of a related pseudogene and relationship to class I major histocompatibility complex genes". Genomics 18 (3): 575–87. doi:10.1016/S0888-7543(05)80359-3. PMID 8307568.
Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
Sánchez LM, López-Otín C, Bjorkman PJ (1997). "Biochemical characterization and crystalization of human Zn-alpha2-glycoprotein, a soluble class I major histocompatibility complex homolog". Proc. Natl. Acad. Sci. U.S.A. 94 (9): 4626–30. doi:10.1073/pnas.94.9.4626. PMC 20774. PMID 9114041. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=20774.
Brysk MM, Lei G, Rajaraman S, et al. (1997). "Gene expression of zinc-alpha 2-glycoprotein in normal human epidermal and buccal epithelia". In Vivo 11 (3): 271–4. PMID 9239523.
Lei G, Arany I, Selvanayagam P, et al. (1997). "Detection and cloning of epidermal zinc-alpha 2-glycoprotein cDNA and expression in normal human skin and in tumors". J. Cell. Biochem. 67 (2): 216–22. doi:10.1002/(SICI)1097-4644(19971101)67:2<216::AID-JCB6>3.0.CO;2-#. PMID 9328826.
Ogikubo O, Maeda T, Yamane T, et al. (1998). "Regulation of Zn-alpha2-glycoprotein-mediated cell adhesion by kininogens and their derivatives". Biochem. Biophys. Res. Commun. 252 (1): 257–62. doi:10.1006/bbrc.1998.9614. PMID 9813179.
Sánchez LM, Chirino AJ, Bjorkman P (1999). "Crystal structure of human ZAG, a fat-depleting factor related to MHC molecules". Science 283 (5409): 1914–9. doi:10.1126/science.283.5409.1914. PMID 10206894.
Lei G, Brysk H, Arany I, et al. (1999). "Characterization of zinc-alpha(2)-glycoprotein as a cell adhesion molecule that inhibits the proliferation of an oral tumor cell line". J. Cell. Biochem. 75 (1): 160–9. doi:10.1002/(SICI)1097-4644(19991001)75:1<160::AID-JCB16>3.0.CO;2-B. PMID 10462714.
Davidsson P, Nilsson CL (2000). "Peptide mapping of proteins in cerebrospinal fluid utilizing a rapid preparative two-dimensional electrophoretic procedure and matrix-assisted laser desorption/ionization mass spectrometry". Biochim. Biophys. Acta 1473 (2–3): 391–9. PMID 10594376.
Kennedy MW, Heikema AP, Cooper A, et al. (2001). "Hydrophobic ligand binding by Zn-alpha 2-glycoprotein, a soluble fat-depleting factor related to major histocompatibility complex proteins". J. Biol. Chem. 276 (37): 35008–13. doi:10.1074/jbc.C100301200. PMID 11425849.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Scherer SW, Cheung J, MacDonald JR, et al. (2003). "Human chromosome 7: DNA sequence and biology". Science 300 (5620): 767–72. doi:10.1126/science.1083423. PMC 2882961. PMID 12690205. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2882961.
Hillier LW, Fulton RS, Fulton LA, et al. (2003). "The DNA sequence of human chromosome 7". Nature 424 (6945): 157–64. doi:10.1038/nature01782. PMID 12853948.
BURGI W, SCHMID K (1998). "Preparation and properties of Zn-alpha 2-glycoprotein of normal human plasma". J. Biol. Chem. 236: 1066–74. PMID 13689030.

PDB gallery

1t7v: Zn-alpha-2-glycoprotein; baculo-ZAG PEG 200

1t7w: Zn-alpha-2-glycoprotein; CHO-ZAG PEG 400

1t7x: Zn-alpha-2-glycoprotein; refolded CHO-ZAG PEG 400

1t7y: Zn-alpha-2-glycoprotein; baculo-ZAG PEG 200, no glycerol

1t7z: Zn-alpha-2-glycoprotein; baculo-ZAG no PEG, no glycerol

1t80: Zn-alpha-2-glycoprotein; CHO-ZAG PEG 200

1zag: HUMAN ZINC-ALPHA-2-GLYCOPROTEIN

2icn:

AZGP1

References

Further reading