ARHGEF1

Rho guanine nucleotide exchange factor (GEF) 1

PDB rendering based on 1iap.

Available structures
PDB	1IAP, 1SHZ

Identifiers

Symbols

ARHGEF1; GEF1; LBCL2; LSC; P115-RHOGEF; SUB1.5

External IDs

OMIM: 601855 MGI: 1353510 HomoloGene: 3454 GeneCards: ARHGEF1 Gene

Gene Ontology
Molecular function	• guanyl-nucleotide exchange factor activity • Rho guanyl-nucleotide exchange factor activity • GTPase activator activity • protein binding
Cellular component	• intracellular • cytoplasm • cytosol • plasma membrane • plasma membrane
Biological process	• Rho protein signal transduction • cell proliferation • regulation of Rho protein signal transduction • nerve growth factor receptor signaling pathway • regulation of axonogenesis • negative regulation of axonogenesis • positive regulation of axonogenesis
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 19:
42.39 – 42.41 Mb

Chr 7:
25.69 – 25.71 Mb

PubMed search

[1]

[2]

Rho guanine nucleotide exchange factor 1 is a protein that in humans is encoded by the ARHGEF1 gene.^[1]^[2]^[3]

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.^[3]

Interactions

ARHGEF1 has been shown to interact with GNA13,^[4]^[5]^[6] CD44,^[4] P110α^[7] and GNA12.^[4]

References

^ Hart MJ, Sharma S, elMasry N, Qiu RG, McCabe P, Polakis P, Bollag G (Nov 1996). "Identification of a novel guanine nucleotide exchange factor for the Rho GTPase". J Biol Chem 271 (41): 25452–8. doi:10.1074/jbc.271.41.25452. PMID 8810315.
^ Aasheim HC, Pedeutour F, Smeland EB (May 1997). "Characterization, expression and chromosomal localization of a human gene homologous to the mouse Lsc oncogene, with strongest expression in hematopoetic tissues". Oncogene 14 (14): 1747–52. doi:10.1038/sj.onc.1200994. PMID 9135076.
^ ^a ^b "Entrez Gene: ARHGEF1 Rho guanine nucleotide exchange factor (GEF) 1". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=9138.
^ ^a ^b ^c
^ Bhattacharyya, Raja; Wedegaertner Philip B (Apr. 2003). "Mutation of an N-terminal acidic-rich region of p115-RhoGEF dissociates alpha13 binding and alpha13-promoted plasma membrane recruitment". FEBS Lett. (Netherlands) 540 (1–3): 211–6. doi:10.1016/S0014-5793(03)00267-9. ISSN 0014-5793. PMID 12681510.
^ Hart, M J; Jiang X, Kozasa T, Roscoe W, Singer W D, Gilman A G, Sternweis P C, Bollag G (Jun. 1998). "Direct stimulation of the guanine nucleotide exchange activity of p115 RhoGEF by Galpha13". Science (UNITED STATES) 280 (5372): 2112–4. doi:10.1126/science.280.5372.2112. ISSN 0036-8075. PMID 9641916.
^ Holinstat, Michael; Mehta Dolly, Kozasa Tohru, Minshall Richard D, Malik Asrar B (Aug. 2003). "Protein kinase Calpha-induced p115RhoGEF phosphorylation signals endothelial cytoskeletal rearrangement". J. Biol. Chem. (United States) 278 (31): 28793–8. doi:10.1074/jbc.M303900200. ISSN 0021-9258. PMID 12754211.

Fukuhara S, Chikumi H, Gutkind JS (2001). "RGS-containing RhoGEFs: the missing link between transforming G proteins and Rho?". Oncogene 20 (13): 1661–8. doi:10.1038/sj.onc.1204182. PMID 11313914.
Kozasa T, Jiang X, Hart MJ et al. (1998). "p115 RhoGEF, a GTPase activating protein for Galpha12 and Galpha13". Science 280 (5372): 2109–11. doi:10.1126/science.280.5372.2109. PMID 9641915.
Hart MJ, Jiang X, Kozasa T et al. (1998). "Direct stimulation of the guanine nucleotide exchange activity of p115 RhoGEF by Galpha13". Science 280 (5372): 2112–4. doi:10.1126/science.280.5372.2112. PMID 9641916.
Zhang H, Wang L, Kao S et al. (1999). "Functional interaction between the cytoplasmic leucine-zipper domain of HIV-1 gp41 and p115-RhoGEF". Curr. Biol. 9 (21): 1271–4. doi:10.1016/S0960-9822(99)80511-9. PMID 10556093.
Kim ST, Lim DS, Canman CE, Kastan MB (2000). "Substrate specificities and identification of putative substrates of ATM kinase family members". J. Biol. Chem. 274 (53): 37538–43. doi:10.1074/jbc.274.53.37538. PMID 10608806.
Bhattacharyya R, Wedegaertner PB (2000). "Galpha 13 requires palmitoylation for plasma membrane localization, Rho-dependent signaling, and promotion of p115-RhoGEF membrane binding". J. Biol. Chem. 275 (20): 14992–9. doi:10.1074/jbc.M000415200. PMID 10747909.
Kozasa T (2001). "Regulation of G protein-mediated signal transduction by RGS proteins". Life Sci. 68 (19–20): 2309–17. doi:10.1016/S0024-3205(01)01020-7. PMID 11358341.
Wells CD, Gutowski S, Bollag G, Sternweis PC (2001). "Identification of potential mechanisms for regulation of p115 RhoGEF through analysis of endogenous and mutant forms of the exchange factor". J. Biol. Chem. 276 (31): 28897–905. doi:10.1074/jbc.M102913200. PMID 11384980.
Chen Z, Wells CD, Sternweis PC, Sprang SR (2001). "Structure of the rgRGS domain of p115RhoGEF". Nat. Struct. Biol. 8 (9): 805–9. doi:10.1038/nsb0901-805. PMID 11524686.
Park B, Nguyen NT, Dutt P et al. (2003). "Association of Lbc Rho guanine nucleotide exchange factor with alpha-catenin-related protein, alpha-catulin/CTNNAL1, supports serum response factor activation". J. Biol. Chem. 277 (47): 45361–70. doi:10.1074/jbc.M202447200. PMID 12270917.
Driessens MH, Olivo C, Nagata K et al. (2002). "B plexins activate Rho through PDZ-RhoGEF". FEBS Lett. 529 (2–3): 168–72. doi:10.1016/S0014-5793(02)03323-9. PMID 12372594.
Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Chen Z, Singer WD, Wells CD et al. (2003). "Mapping the Galpha13 binding interface of the rgRGS domain of p115RhoGEF". J. Biol. Chem. 278 (11): 9912–9. doi:10.1074/jbc.M212695200. PMID 12525488.
Bhattacharyya R, Wedegaertner PB (2003). "Characterization of G alpha 13-dependent plasma membrane recruitment of p115RhoGEF". Biochem. J. 371 (Pt 3): 709–20. doi:10.1042/BJ20021897. PMC 1223324. PMID 12534370. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1223324.
Bhattacharyya R, Wedegaertner PB (2003). "Mutation of an N-terminal acidic-rich region of p115-RhoGEF dissociates alpha13 binding and alpha13-promoted plasma membrane recruitment". FEBS Lett. 540 (1–3): 211–6. doi:10.1016/S0014-5793(03)00267-9. PMID 12681510.
Bourguignon LY, Singleton PA, Zhu H, Diedrich F (2003). "Hyaluronan-mediated CD44 interaction with RhoGEF and Rho kinase promotes Grb2-associated binder-1 phosphorylation and phosphatidylinositol 3-kinase signaling leading to cytokine (macrophage-colony stimulating factor) production and breast tumor progression". J. Biol. Chem. 278 (32): 29420–34. doi:10.1074/jbc.M301885200. PMID 12748184.
Holinstat M, Mehta D, Kozasa T et al. (2003). "Protein kinase Calpha-induced p115RhoGEF phosphorylation signals endothelial cytoskeletal rearrangement". J. Biol. Chem. 278 (31): 28793–8. doi:10.1074/jbc.M303900200. PMID 12754211.

PDB gallery

1iap: CRYSTAL STRUCTURE OF P115RHOGEF RGRGS DOMAIN

1shz: Crystal Structure of the p115RhoGEF rgRGS Domain in A Complex with Galpha(13):Galpha(i1) Chimera

ARHGEF1

Interactions

References

Further reading