ARFGAP1

ADP-ribosylation factor GTPase activating protein 1

Rendering based on PDB 3DWD.

Available structures
PDB	3DWD, 3O47

Identifiers

Symbols

ARFGAP1; ARF1GAP; HRIHFB2281; MGC39924

External IDs

OMIM: 608377 MGI: 2183559 HomoloGene: 5517 GeneCards: ARFGAP1 Gene

Gene Ontology
Molecular function	• ARF GTPase activator activity • zinc ion binding • metal ion binding
Cellular component	• cytoplasm • Golgi apparatus • cytosol • cytosol • Golgi-associated vesicle membrane
Biological process	• retrograde vesicle-mediated transport, Golgi to ER • protein transport • cellular membrane organization • vesicle-mediated transport • regulation of ARF GTPase activity • COPI coating of Golgi vesicle
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 20:
61.9 – 61.92 Mb

Chr 2:
180.7 – 180.72 Mb

PubMed search

[1]

[2]

ADP-ribosylation factor GTPase-activating protein 1 is an enzyme that in humans is encoded by the ARFGAP1 gene.^[1]^[2]

The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene.^[2]

Interactions

ARFGAP1 has been shown to interact with KDELR1.^[3]^[4]

References

^ Huber I, Rotman M, Pick E, Makler V, Rothem L, Cukierman E, Cassel D (Feb 2001). "Expression, purification, and properties of ADP-ribosylation factor (ARF) GTPase activating protein-1". Methods Enzymol 329: 307–16. doi:10.1016/S0076-6879(01)29092-2. PMID 11210549.
^ ^a ^b "Entrez Gene: ARFGAP1 ADP-ribosylation factor GTPase activating protein 1". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=55738.
^ Aoe, T; Cukierman E, Lee A, Cassel D, Peters P J, Hsu V W (Dec. 1997). "The KDEL receptor, ERD2, regulates intracellular traffic by recruiting a GTPase-activating protein for ARF1". EMBO J. (ENGLAND) 16 (24): 7305–16. doi:10.1093/emboj/16.24.7305. ISSN 0261-4189. PMC 1170331. PMID 9405360. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1170331.
^ Majoul, I; Straub M, Hell S W, Duden R, Söling H D (Jul. 2001). "KDEL-cargo regulates interactions between proteins involved in COPI vesicle traffic: measurements in living cells using FRET". Dev. Cell (United States) 1 (1): 139–53. doi:10.1016/S1534-5807(01)00004-1. ISSN 1534-5807. PMID 11703931.

Rothman JE (1994). "Mechanisms of intracellular protein transport.". Nature 372 (6501): 55–63. doi:10.1038/372055a0. PMID 7969419.
Bernards A, Settleman J (2005). "GAP control: regulating the regulators of small GTPases.". Trends Cell Biol. 14 (7): 377–85. doi:10.1016/j.tcb.2004.05.003. PMID 15246431.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Cukierman E, Huber I, Rotman M, Cassel D (1996). "The ARF1 GTPase-activating protein: zinc finger motif and Golgi complex localization". Science 270 (5244): 1999–2002. doi:10.1126/science.270.5244.1999. PMID 8533093.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Aoe T, Cukierman E, Lee A, et al. (1998). "The KDEL receptor, ERD2, regulates intracellular traffic by recruiting a GTPase-activating protein for ARF1". EMBO J. 16 (24): 7305–16. doi:10.1093/emboj/16.24.7305. PMC 1170331. PMID 9405360. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1170331.
Mossessova E, Gulbis JM, Goldberg J (1998). "Structure of the guanine nucleotide exchange factor Sec7 domain of human arno and analysis of the interaction with ARF GTPase". Cell 92 (3): 415–23. doi:10.1016/S0092-8674(00)80933-2. PMID 9476900.
Huber I, Cukierman E, Rotman M, et al. (1998). "Requirement for both the amino-terminal catalytic domain and a noncatalytic domain for in vivo activity of ADP-ribosylation factor GTPase-activating protein". J. Biol. Chem. 273 (38): 24786–91. doi:10.1074/jbc.273.38.24786. PMID 9733781.
Zhang C, Yu Y, Zhang S, et al. (2000). "Characterization, chromosomal assignment, and tissue expression of a novel human gene belonging to the ARF GAP family". Genomics 63 (3): 400–8. doi:10.1006/geno.1999.6095. PMID 10704287.
Majoul I, Straub M, Hell SW, et al. (2001). "KDEL-cargo regulates interactions between proteins involved in COPI vesicle traffic: measurements in living cells using FRET". Dev. Cell 1 (1): 139–53. doi:10.1016/S1534-5807(01)00004-1. PMID 11703931.
Deloukas P, Matthews LH, Ashurst J, et al. (2002). "The DNA sequence and comparative analysis of human chromosome 20". Nature 414 (6866): 865–71. doi:10.1038/414865a. PMID 11780052.
Yu S, Roth MG (2003). "Casein kinase I regulates membrane binding by ARF GAP1". Mol. Biol. Cell 13 (8): 2559–70. doi:10.1091/mbc.E02-04-0189. PMC 117925. PMID 12181329. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=117925.
Yang JS, Lee SY, Gao M, et al. (2002). "ARFGAP1 promotes the formation of COPI vesicles, suggesting function as a component of the coat". J. Cell Biol. 159 (1): 69–78. doi:10.1083/jcb.200206015. PMC 2173491. PMID 12379802. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2173491.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=514446.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.

ARFGAP1

Interactions

References

Further reading