AP2B1
AP-2 complex subunit beta is a protein that in humans is encoded by the AP2B1 gene.[1][2][3]
The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene.[3]
Interactions
AP2B1 has been shown to interact with TGF beta receptor 2,[4] AP1M2,[5] BUB1B,[6] LDLRAP1[5][7] and Arrestin beta 2.[8][9]
References
- ^ Gallusser A, Kirchhausen T (Jan 1994). "The beta 1 and beta 2 subunits of the AP complexes are the clathrin coat assembly components". EMBO J 12 (13): 5237–44. PMC 413789. PMID 8262066. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=413789.
- ^ Druck T, Gu Y, Prabhala G, Cannizzaro LA, Park SH, Huebner K, Keen JH (Apr 1996). "Chromosome localization of human genes for clathrin adaptor polypeptides AP2 beta and AP50 and the clathrin-binding protein, VCP". Genomics 30 (1): 94–7. doi:10.1006/geno.1995.0016. PMID 8595912.
- ^ a b "Entrez Gene: AP2B1 adaptor-related protein complex 2, beta 1 subunit". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=163.
- ^ Yao, Diying; Ehrlich Marcelo, Henis Yoav I, Leof Edward B (Nov. 2002). "Transforming growth factor-beta receptors interact with AP2 by direct binding to beta2 subunit". Mol. Biol. Cell (United States) 13 (11): 4001–12. doi:10.1091/mbc.02-07-0104. ISSN 1059-1524. PMC 133610. PMID 12429842. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=133610.
- ^ a b Rual, Jean-François; Venkatesan Kavitha, Hao Tong, Hirozane-Kishikawa Tomoko, Dricot Amélie, Li Ning, Berriz Gabriel F, Gibbons Francis D, Dreze Matija, Ayivi-Guedehoussou Nono, Klitgord Niels, Simon Christophe, Boxem Mike, Milstein Stuart, Rosenberg Jennifer, Goldberg Debra S, Zhang Lan V, Wong Sharyl L, Franklin Giovanni, Li Siming, Albala Joanna S, Lim Janghoo, Fraughton Carlene, Llamosas Estelle, Cevik Sebiha, Bex Camille, Lamesch Philippe, Sikorski Robert S, Vandenhaute Jean, Zoghbi Huda Y, Smolyar Alex, Bosak Stephanie, Sequerra Reynaldo, Doucette-Stamm Lynn, Cusick Michael E, Hill David E, Roth Frederick P, Vidal Marc (Oct. 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature (England) 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
- ^ Cayrol, Corinne; Cougoule Céline, Wright Michel (Nov. 2002). "The beta2-adaptin clathrin adaptor interacts with the mitotic checkpoint kinase BubR1". Biochem. Biophys. Res. Commun. (United States) 298 (5): 720–30. doi:10.1016/S0006-291X(02)02522-6. ISSN 0006-291X. PMID 12419313.
- ^ He, Guocheng; Gupta Sarita, Yi Ming, Michaely Peter, Hobbs Helen H, Cohen Jonathan C (Nov. 2002). "ARH is a modular adaptor protein that interacts with the LDL receptor, clathrin, and AP-2". J. Biol. Chem. (United States) 277 (46): 44044–9. doi:10.1074/jbc.M208539200. ISSN 0021-9258. PMID 12221107.
- ^ Laporte, S A; Oakley R H, Zhang J, Holt J A, Ferguson S S, Caron M G, Barak L S (Mar. 1999). "The beta2-adrenergic receptor/betaarrestin complex recruits the clathrin adaptor AP-2 during endocytosis". Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 96 (7): 3712–7. doi:10.1073/pnas.96.7.3712. ISSN 0027-8424. PMC 22359. PMID 10097102. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=22359.
- ^ Kim, You-Me; Benovic Jeffrey L (Aug. 2002). "Differential roles of arrestin-2 interaction with clathrin and adaptor protein 2 in G protein-coupled receptor trafficking". J. Biol. Chem. (United States) 277 (34): 30760–8. doi:10.1074/jbc.M204528200. ISSN 0021-9258. PMID 12070169.
Further reading
- Kirchhausen T (2000). "Clathrin". Annu. Rev. Biochem. 69: 699–727. doi:10.1146/annurev.biochem.69.1.699. PMID 10966473.
- Geyer M, Fackler OT, Peterlin BM (2001). "Structure--function relationships in HIV-1 Nef". EMBO Rep. 2 (7): 580–5. doi:10.1093/embo-reports/kve141. PMC 1083955. PMID 11463741. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1083955.
- Ponnambalam S, Robinson MS, Jackson AP, et al. (1990). "Conservation and diversity in families of coated vesicle adaptins". J. Biol. Chem. 265 (9): 4814–20. PMID 1969413.
- Greenberg ME, Bronson S, Lock M, et al. (1998). "Co-localization of HIV-1 Nef with the AP-2 adaptor protein complex correlates with Nef-induced CD4 down-regulation". EMBO J. 16 (23): 6964–76. doi:10.1093/emboj/16.23.6964. PMC 1170300. PMID 9384576. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1170300.
- Lim DS, Kirsch DG, Canman CE, et al. (1998). "ATM binds to beta-adaptin in cytoplasmic vesicles". Proc. Natl. Acad. Sci. U.S.A. 95 (17): 10146–51. doi:10.1073/pnas.95.17.10146. PMC 21476. PMID 9707615. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=21476.
- Greenberg M, DeTulleo L, Rapoport I, et al. (1999). "A dileucine motif in HIV-1 Nef is essential for sorting into clathrin-coated pits and for downregulation of CD4". Curr. Biol. 8 (22): 1239–42. doi:10.1016/S0960-9822(07)00518-0. PMID 9811611.
- Berlioz-Torrent C, Shacklett BL, Erdtmann L, et al. (1999). "Interactions of the cytoplasmic domains of human and simian retroviral transmembrane proteins with components of the clathrin adaptor complexes modulate intracellular and cell surface expression of envelope glycoproteins". J. Virol. 73 (2): 1350–61. PMC 103959. PMID 9882340. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=103959.
- Owen DJ, Vallis Y, Pearse BM, et al. (2000). "The structure and function of the beta 2-adaptin appendage domain". EMBO J. 19 (16): 4216–27. doi:10.1093/emboj/19.16.4216. PMC 302036. PMID 10944104. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=302036.
- Fan GH, Yang W, Wang XJ, et al. (2001). "Identification of a motif in the carboxyl terminus of CXCR2 that is involved in adaptin 2 binding and receptor internalization". Biochemistry 40 (3): 791–800. doi:10.1021/bi001661b. PMC 2664867. PMID 11170396. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2664867.
- Swigut T, Shohdy N, Skowronski J (2001). "Mechanism for down-regulation of CD28 by Nef". EMBO J. 20 (7): 1593–604. doi:10.1093/emboj/20.7.1593. PMC 145469. PMID 11285224. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=145469.
- Laporte SA, Miller WE, Kim KM, Caron MG (2002). "beta-Arrestin/AP-2 interaction in G protein-coupled receptor internalization: identification of a beta-arrestin binging site in beta 2-adaptin". J. Biol. Chem. 277 (11): 9247–54. doi:10.1074/jbc.M108490200. PMID 11777907.
- Collins BM, McCoy AJ, Kent HM, et al. (2002). "Molecular architecture and functional model of the endocytic AP2 complex". Cell 109 (4): 523–35. doi:10.1016/S0092-8674(02)00735-3. PMID 12086608.
- He G, Gupta S, Yi M, et al. (2003). "ARH is a modular adaptor protein that interacts with the LDL receptor, clathrin, and AP-2". J. Biol. Chem. 277 (46): 44044–9. doi:10.1074/jbc.M208539200. PMID 12221107.
- Cayrol C, Cougoule C, Wright M (2003). "The beta2-adaptin clathrin adaptor interacts with the mitotic checkpoint kinase BubR1". Biochem. Biophys. Res. Commun. 298 (5): 720–30. doi:10.1016/S0006-291X(02)02522-6. PMID 12419313.
- Yao D, Ehrlich M, Henis YI, Leof EB (2003). "Transforming growth factor-beta receptors interact with AP2 by direct binding to beta2 subunit". Mol. Biol. Cell 13 (11): 4001–12. doi:10.1091/mbc.02-07-0104. PMC 133610. PMID 12429842. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=133610.
- Kalthoff C, Groos S, Kohl R, et al. (2003). "Clint: a novel clathrin-binding ENTH-domain protein at the Golgi". Mol. Biol. Cell 13 (11): 4060–73. doi:10.1091/mbc.E02-03-0171. PMC 133614. PMID 12429846. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=133614.
|
PDB gallery
|
|
|
|
1e42: BETA2-ADAPTIN APPENDAGE DOMAIN, FROM CLATHRIN ADAPTOR AP2
|
|
|
1w63: AP1 CLATHRIN ADAPTOR CORE
|
|
|
2g30: beta appendage of AP2 complexed with ARH peptide
|
|
|
2iv9: B2-APPENDAGE FROM AP2 IN COMPLEX WITH EPS15 PEPTIDE
|
|
|
|