Liver function tests

Liver function tests
Intervention
MeSH	D008111

Liver function tests (LFTs or LFs), are groups of clinical biochemistry laboratory blood assays designed to give information about the state of a patient's liver.^[1] The parameters measured include PT/INR, aPTT, albumin, billirubin (direct and indirect) and others. According to some, liver transaminases (AST/ALT (SGOT/SGPT) are not liver function tests, but are biomarkers of liver injury in a patient with some degree of intact liver function. Other sources include transaminases.^[2]^[3] Most liver diseases cause only mild symptoms initially, but it is vital that these diseases be detected early. Hepatic (liver) involvement in some diseases can be of crucial importance. This testing is performed by a medical technologist on a patient's serum or plasma sample obtained by phlebotomy. Some tests are associated with functionality (e.g., albumin); some with cellular integrity (e.g., transaminase) and some with conditions linked to the biliary tract (gamma-glutamyl transferase and alkaline phosphatase). Several biochemical tests are useful in the evaluation and management of patients with hepatic dysfunction. These tests can be used to (1) detect the presence of liver disease, (2) distinguish among different types of liver disorders, (3) gauge the extent of known liver damage, and (4) follow the response to treatment. Some or all of these measurements are also carried out (usually about twice a year for routine cases) on those individuals taking certain medications- anticonvulsants are a notable example- in order to ensure that the medications are not damaging the person's liver.

1 Standard liver panel
2 Other tests commonly requested alongside LFTs
3 See also
4 References
5 External links

Standard liver panel

Albumin (Alb)

Reference range
3.5 to 5.3 g/dL

Albumin is a protein made specifically by the liver, and can be measured cheaply and easily. It is the main constituent of total protein; the remaining fraction is called globulin (including the immunoglobulins). Albumin levels are decreased in chronic liver disease, such as cirrhosis. It is also decreased in nephrotic syndrome, where it is lost through the urine. Poor nutrition or states of impaired protein catabolism, such as in Ménétrier's disease, may also lead to hypoalbuminaemia. The half-life of albumin is approximately 20 days. Albumin is not considered to be an especially useful marker of liver synthetic function; coagulation factors (see below) are much more sensitive

Alanine transaminase (ALT)

Reference range
9 to 52 IU/L^[4]

Alanine transaminase (ALT), also called Serum Glutamic Pyruvate Transaminase (SGPT) or Alanine aminotransferase (ALAT) is an enzyme present in hepatocytes (liver cells). When a cell is damaged, it leaks this enzyme into the blood, where it is measured. ALT rises dramatically in acute liver damage, such as viral hepatitis or paracetamol (acetaminophen) overdose. Elevations are often measured in multiples of the upper limit of normal (ULN).

Aspartate transaminase (AST)

Reference range
10 to 35 IU/L^[4]

Aspartate transaminase (AST) also called Serum Glutamic Oxaloacetic Transaminase (SGOT) or aspartate aminotransferase (ASAT) is similar to ALT in that it is another enzyme associated with liver parenchymal cells. It is raised in acute liver damage, but is also present in red blood cells, and cardiac and skeletal muscle and is therefore not specific to the liver. The ratio of AST to ALT is sometimes useful in differentiating between causes of liver damage.^[5]^[6] Elevated AST levels are not specific for liver damage, and AST has also been used as a cardiac marker.

Alkaline phosphatase (ALP)

Reference range
30 to 120 IU/L^[4]

Alkaline phosphatase (ALP) is an enzyme in the cells lining the biliary ducts of the liver. ALP levels in plasma will rise with large bile duct obstruction, intrahepatic cholestasis or infiltrative diseases of the liver. ALP is also present in bone and placental tissue, so it is higher in growing children (as their bones are being remodelled) and elderly patients with Paget's disease.

Total bilirubin (TBIL)

Reference range
0.2–1.2 mg/dL

Bilirubin is a breakdown product of heme (a part of hemoglobin in red blood cells). The liver is responsible for clearing the blood of bilirubin. It does this by the following mechanism: Bilirubin is taken up into hepatocytes, conjugated (modified to make it water-soluble), and secreted into the bile, which is excreted into the intestine.

Increased total bilirubin causes jaundice, and can signal a number of problems:

1. Prehepatic: Increased bilirubin production. This can be due to a number of causes, including hemolytic anemias and internal hemorrhage.
2. Hepatic: Problems with the liver, which are reflected as deficiencies in bilirubin metabolism (e.g., reduced hepatocyte uptake, impaired conjugation of bilirubin, and reduced hepatocyte secretion of bilirubin). Some examples would be cirrhosis and viral hepatitis.
3. Posthepatic: Obstruction of the bile ducts, reflected as deficiencies in bilirubin excretion. (Obstruction can be located either within the liver or in the bile duct).

Direct bilirubin (Conjugated Bilirubin)

Reference range
0.1–0.4 mg/dL

The diagnosis is narrowed down further by looking at the levels of direct bilirubin.

If direct (i.e. conjugated) bilirubin is normal, then the problem is an excess of unconjugated bilirubin, and the location of the problem is upstream of bilirubin excretion. Hemolysis, viral hepatitis, or cirrhosis can be suspected.
If direct bilirubin is elevated, then the liver is conjugating bilirubin normally, but is not able to excrete it. Bile duct obstruction by gallstones or cancer should be suspected.

Gamma glutamyl transpeptidase (GGT)

Reference range
0 to 42 IU/L^[4]

Although reasonably specific to the liver and a more sensitive marker for cholestatic damage than ALP, Gamma glutamyl transpeptidase (GGT) may be elevated with even minor, sub-clinical levels of liver dysfunction. It can also be helpful in identifying the cause of an isolated elevation in ALP (GGT is raised in chronic alcohol toxicity).

Other tests commonly requested alongside LFTs

Pathophysiology sample values
BMP/ELECTROLYTES:
Na⁺=140	Cl⁻=100	BUN=20	/
			Glu=150
K⁺=4	CO₂=22	PCr=1.0	\
ARTERIAL BLOOD GAS:
HCO₃^-=24	p_aCO₂=40	p_aO₂=95	pH=7.40
ALVEOLAR GAS:
	p_ACO₂=36	p_AO₂=105	A-a g=10
OTHER:
Ca=9.5	Mg²⁺=2.0	PO₄=1
CK=55	BE=−0.36	AG=16
SERUM OSMOLARITY/RENAL:
PMO = 300	PCO=295	POG=5	BUN:Cr=20
URINALYSIS:
UNa⁺=80	UCl⁻=100	UAG=5	FENa=0.95
UK⁺=25	USG=1.01	UCr=60	UO=800
PROTEIN/GI/LIVER FUNCTION TESTS:
LDH=100	TP=7.6	AST=25	TBIL=0.7
ALP=71	Alb=4.0	ALT=40	BC=0.5
		AST/ALT=0.6	BU=0.2
AF alb=3.0	SAAG=1.0		SOG=60
CSF:
CSF alb=30	CSF glu=60	CSF/S alb=7.5	CSF/S glu=0.4

5' Nucleotidase (5'NTD)

5' Nucleotidase is another test specific for cholestasis or damage to the intra or extrahepatic biliary system, and in some laboratories, is used as a substitute for GGT for ascertaining whether an elevated ALP is of biliary or extra-biliary origin.

Coagulation test (e.g., INR)

The liver is responsible for the production of coagulation factors. The international normalized ratio (INR) measures the speed of a particular pathway of coagulation, comparing it to normal. Increased levels of INR means that blood is taking more time than usual to coagulate or clot. The INR will be increased only if the liver is so damaged that synthesis of vitamin K-dependent coagulation factors has been impaired; it is not a sensitive measure of liver function.

It is very important to normalize the INR before operating on people with liver problems (usually by transfusion with blood plasma containing the deficient factors) as they could bleed excessively.