Herpesviridae

Herpesviridae
Virus classification
Group: Group I (dsDNA)
Order: Herpesvirales
Family: Herpesviridae
Genera

Subfamily Alphaherpesvirinae

  • Iltovirus
  • Mardivirus
  • Simplexvirus
  • Unassigned
  • Varicellovirus

Subfamily Betaherpesvirinae

Subfamily Gammaherpesvirinae

  • Lymphocryptovirus
  • Macavirus
  • Percavirus
  • Rhadinovirus
  • Unassigned

Subfamily Unassigned

  • Unassigned

The Herpesviridae are a large family of DNA viruses that cause diseases in animals, including humans.[1][2][3] The members of this family are also known as herpesviruses. The family name is derived from the Greek word herpein ("to creep"), referring to the latent, recurring infections typical of this group of viruses. Herpesviridae can cause latent or lytic infections.

Contents

Viral structure

Herpesviruses all share a common structure—all herpesviruses are composed of relatively large double-stranded, linear DNA genomes encoding 100-200 genes encased within an icosahedral protein cage called the capsid which is itself wrapped in a protein layer called the tegument containing both viral proteins and viral mRNAs and a lipid bilayer membrane called the envelope. This whole particle is known as the virion.

Herpes virus life-cycle

All Herpesviruses are nuclear-replicating—the viral DNA is transcribed to RNA within the infected cell's nucleus.

Infection is initiated when a viral particle contacts a cell with specific types of receptor molecules on the cell surface. Following binding of viral envelope glycoproteins to cell membrane receptors, the virion is internalized and dismantled, allowing viral DNA to migrate to the cell nucleus. Within the nucleus, replication of viral DNA and transcription of viral genes occurs.

During symptomatic infection, infected cells transcribe lytic viral genes. In some host cells, a small number of viral genes termed latency associated transcript (LAT) accumulate instead. In this fashion the virus can persist in the cell (and thus the host) indefinitely. While primary infection is often accompanied by a self-limited period of clinical illness, long-term latency is symptom-free.

Reactivation of latent viruses has been implicated in a number of diseases (e.g. Shingles, Pityriasis Rosea). Following activation, transcription of viral genes transitions from latency-associated LAT to multiple lytic genes; these lead to enhanced replication and virus production. Often, lytic activation leads to cell death. Clinically, lytic activation is often accompanied by emergence of non-specific symptoms such as low grade fever, headache, sore throat, malaise, and rash as well as clinical signs such as swollen or tender lymph nodes and immunological findings such as reduced levels of natural killer cells.

Immune system evasions

Herpesviruses are known for their ability to establish lifelong infections. One way this is possible is through immune evasion. Herpesviruses have found many different ways to evade the immune system. One such way is by encoding a protein mimicking human interleukin 10 (hIL-10) and another is by downregulation of the Major Histocompatibility Complex II (MHC II) in infected cells. Research conducted on cytomegalovirus (CMV) indicates that the viral human IL-10 homolog, cmvIL-10, is important in inhibiting pro-inflammatory cytokine synthesis. The cmvIL-10 protein has 27% identity with hIL-10 and only one conserved residue out of the nine amino acids that make up the functional site for cytokine synthesis inhibition on hIL-10. There is, however, much similarity in the functions of hIL-10 and cmvIL-10. Both have been shown to down regulate IFN-γ, IL-1α, GM-CSF, IL-6 and TNF- α, which are all pro-inflammatory cytokines. They have also been shown to play a role in downregulating MHC I and MHC II and up regulating HLA-G (non-classical MHC I). These two events allow for immune evasion by suppressing the cell-mediated immune response and natural killer cell response, respectively. The similarities between hIL-10 and cmvIL-10 may be explained by the fact that hIL-10 and cmvIL-10 both use the same cell surface receptor, the hIL-10 receptor. One difference in the function of hIL-10 and cmvIL-10 is that hIL-10 causes human peripheral blood mononuclear cells (PBMC) to both increase and decrease in proliferation whereas cmvIL-10 only causes a decrease in proliferation of PBMCs. This indicates that cmvIL-10 may lack the stimulatory effects that hIL-10 has on these cells.[4]

It was found that cmvIL-10 functions through phosphorylation of the Stat3 protein. It was originally thought that this phosphorylation was a result of the JAK-STAT pathway. However, despite evidence that JAK does indeed phosphorylate Stat3, its inhibition has no significant influence on cytokine synthesis inhibition. Another protein, PI3K, was also found to phosphorylate Stat3. PI3K inhibition, unlike JAK inhibition, did have a significant impact on cytokine synthesis. The difference between PI3K and JAK in Stat3 phosphorylation is that PI3K phosphorylates Stat3 on the S727 residue whereas JAK phosphorylates Stat3 on the Y705 residue. This difference in phosphorylation positions seems to be the key factor in Stat3 activation leading to inhibition of pro-inflammatory cytokine synthesis. In fact, when a PI3K inhibitor is added to cells, the cytokine synthesis levels are significantly restored. The fact that cytokine levels are not completely restored indicates there is another pathway activated by cmvIL-10 that is inhibiting cytokine synthesis. The proposed mechanism is that cmvIL-10 activates PI3K which in turn activates PKB (Akt). PKB may then activate mTOR, which may target Stat3 for phosphorylation on the S727 residue.[5]

Another one of the many ways in which herpes viruses evade the immune system is by down regulation of MHC I and MHC II. This is observed in almost every human herpesvirus. Down regulation of MHC I and MHC II can come about by many different mechanisms, most causing the MHC to be absent from the cell surface. As discussed above, one way is by a viral chemokine homolog such as IL-10. Another mechanism to down regulate MHCs is to encode viral proteins that detain the newly formed MHC in the endoplasmic reticulum (ER). The MHC cannot reach the cell surface and therefore cannot activate the T cell response. The MHCs can also be targeted for destruction in the proteasome or lysosome. The ER protein TAP also plays a role in MHC down regulation. Viral proteins inhibit TAP preventing the MHC from picking up a viral antigen peptide. This prevents proper folding of the MHC and therefore the MHC does not reach the cell surface.[6]

It is important to note that HLA-G is often up regulated in addition to downregulation of MHC I and MHC II. This prevents the natural killer cell response.

Human herpesviridae infections

There are eight distinct viruses in this family known to cause disease in humans.[7][8]

Human Herpesvirus (HHV) classification[1][7]
Type Synonym Subfamily Primary Target Cell Pathophysiology Site of Latency Means of Spread
HHV-1 Herpes simplex virus-1 (HSV-1) α (Alpha) Mucoepithelial Oral and/or genital herpes (predominantly orofacial), as well as other herpes simplex infections Neuron Close contact
HHV-2 Herpes simplex virus-2 (HSV-2) α Mucoepithelial Oral and/or genital herpes (predominantly genital), as well as other herpes simplex infections Neuron Close contact (sexually transmitted disease)
HHV-3 Varicella zoster virus (VZV) α Mucoepithelial Chickenpox and shingles Neuron Respiratory and close contact
HHV-4 Epstein-Barr virus (EBV), lymphocryptovirus γ (Gamma) B cells and epithelial cells Infectious mononucleosis, Burkitt's lymphoma, CNS lymphoma in AIDS patients,
post-transplant lymphoproliferative syndrome (PTLD), nasopharyngeal carcinoma, HIV-associated hairy leukoplakia		 B cell Close contact, transfusions, tissue transplant, and congenital
HHV-5 Cytomegalovirus (CMV) β (Beta) Monocyte, lymphocyte, and epithelial cells Infectious mononucleosis-like syndrome,[9] retinitis, etc. Monocyte, lymphocyte, and ? Saliva
HHV-6 Roseolovirus, Herpes lymphotropic virus β T cells and ? Sixth disease (roseola infantum or exanthem subitum) T cells and ? Respiratory and close contact?
HHV-7 Roseolovirus β T cells and ? Sixth disease (roseola infantum or exanthem subitum) T cells and ?  ?
HHV-8 Kaposi's sarcoma-associated herpesvirus
(KSHV), a type of rhadinovirus		 γ Lymphocyte and other cells Kaposi's sarcoma, primary effusion lymphoma, some types of multicentric Castleman's disease B cell Close contact (sexual), saliva?


Herpesviruses of other animals

In addition to the herpesviruses considered endemic in humans, some viruses associated primarily with animals may infect humans. These are zoonotic infections:

Zoonotic Herpesviruses
Species Type Synonym Subfamily Human Pathophysiology
Macaque monkey CeHV-1 Cercopithecine herpesvirus-1, (Monkey B virus) α Very unusual, with only approximately 25 human cases reported.[10] Untreated infection is often deadly; sixteen of the 25 cases resulted in fatal encephalomyelitis. At least four cases resulted in survival with severe neurologic impairment.[10][11] Symptom awareness and early treatment are important for laboratory workers facing exposure.[12]
Mouse MuHV-4 Murine gammaherpesvirus-68 (MHV-68) γ Zoonotic infection found in 4.5% of general population and more common in laboratory workers handling infected mice.[13] ELISA tests show factor-of-four (x4) false positive results, due to antibody cross-reaction with other Herpes viruses.[13]


Animal herpesviridae

In animal virology the most important herpesviruses belong to the Alphaherpesvirinae. Research on pseudorabies virus (PrV), the causative agent of Aujeszky's disease in pigs, has pioneered animal disease control with genetically modified vaccines. PrV is now extensively studied as a model for basic processes during lytic herpesvirus infection, and for unravelling molecular mechanisms of herpesvirus neurotropism, whereas bovine herpesvirus 1, the causative agent of bovine infectious rhinotracheitis and pustular vulvovaginitis, is analyzed to elucidate molecular mechanisms of latency. The avian infectious laryngotracheitis virus is phylogenetically distant from these two viruses and serves to underline similarity and diversity within the Alphaherpesvirinae.[2][3]

Taxonomy

The family Herpesviridae has been elevated to the order Herpesvirales. This order currently has 3 families, 3 subfamilies plus 1 unassigned, 17 genera plus 4 unassigned, and 90 species.[15]

The following genera are included in the family Herpesviridae:

Research

Research is currently ongoing into a variety of side-effect or co-conditions related to the herpesviruses. These include:

References

  1. ↑ 1.0 1.1 Ryan KJ; Ray CG (editors) (2004). Sherris Medical Microbiology (4th ed.). McGraw Hill. ISBN 0838585299. 
  2. ↑ 2.0 2.1 Mettenleiter et al. (2008). "Molecular Biology of Animal Herpesviruses". Animal Viruses: Molecular Biology. Caister Academic Press. ISBN 978-1-904455-22-6. ISBN 1904455220. http://www.horizonpress.com/avir. 
  3. ↑ 3.0 3.1 Sandri-Goldin RM (editor). (2006). Alpha Herpesviruses: Molecular and Cellular Biology. Caister Academic Press. ISBN 978-1-904455-09-7 . http://www.horizonpress.com/ahv. 
  4. ↑ Spencer, Juliet; et al (Feb. 2002). "Potent Immunosuppressive Activities of Cytomegalovirus-Encoded Interleukin-10". Journal of Virology 76 (3): 1285–1292. PMID 11773404. 
  5. ↑ Spencer, JV (2007). "The Cytomegalovirus Homolog of Interleukin-10 Requires Phosphatidylinositol 3-Kinase Activity for Inhibition of Cytokine Synthesis in Monocytes". Journal of Virology 81 (4): 2083–2086. doi:10.1128/JVI.01655-06. PMID 17121792. 
  6. ↑ Lin, Aifen; Huihui Xu and Weihua Yan (April 2007). "Modulation of HLA Expression in Human Cytomegalovirus Immune Evasion". Cellular and Molecular Immunology 4 (2): 91–98. PMID 17484802. 
  7. ↑ 7.0 7.1 Whitley RJ (1996). Herpesviruses. in: Baron's Medical Microbiology (Baron S et al., eds.) (4th ed.). Univ of Texas Medical Branch. ISBN 0-9631172-1-1. http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=mmed.chapter.3567. 
  8. ↑ Murray PR; Rosenthal KS; Pfaller MA (2005). Medical Microbiology (5th ed.). Elsevier Mosby. ISBN 978-0-323-03303-9; ISBN 0-323-03303-2. 
  9. ↑ Bottieau E, Clerinx J, Van den Enden E, Van Esbroeck M, Colebunders R, Van Gompel A, Van den Ende J (2006). "Infectious mononucleosis-like syndromes in febrile travelers returning from the tropics.". J Travel Med 13 (4): 191–7. doi:10.1111/j.1708-8305.2006.00049.x. PMID 16884400. 
  10. ↑ 10.0 10.1 Weigler BJ (February 1992). "Biology of B virus in macaque and human hosts: a review". Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 14 (2): 555–67. PMID 1313312. 
  11. ↑ Huff J, Barry P (2003). "B-virus (Cercopithecine herpesvirus 1) infection in humans and macaques: potential for zoonotic disease". Emerg Infect Dis 9 (2): 246–50. PMID 12603998. 
  12. ↑ Herpes-B Fact Sheet
  13. ↑ 13.0 13.1 Hricova M, Mistrikova J (2007). "Murine gammaherpesvirus 68 serum antibodies in general human population". Acta virologica 51 (4): 283–7. PMID 18197737. 
  14. ↑ Fenner, Frank J.; Gibbs, E. Paul J.; Murphy, Frederick A.; Rott, Rudolph; Studdert, Michael J.; White, David O. (1993). Veterinary Virology (2nd ed.). Academic Press, Inc. ISBN 0-12-253056-X. 
  15. ↑ Davison AJ (2010) Herpesvirus systematics. Vet. Microbiol.

External links

Microbiology: Virus
Components
Viral envelope · Capsid · Viral protein
Viral life cycle
Viral entry · Viral replication · Viral shedding · Virus latency
Genetics
Reassortment · Antigenic shift · Antigenic drift · Phenotype mixing
Other
Virus disease · Laboratory diagnosis of viral infections · Antiviral drug · Bacteriophage · Neurotropic virus · Oncovirus · History of viruses

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