Xyloglucan-specific endo-beta-1,4-glucanase

From Wikipedia, the free encyclopedia

In enzymology, a xyloglucan-specific endo-beta-1,4-glucanase (EC 3.2.1.151) is an enzyme that catalyzes the chemical reaction

xyloglucan + H₂O $\rightleftharpoons$ xyloglucan oligosaccharides

Thus, the two substrates of this enzyme are xyloglucan and H₂O, whereas its product is xyloglucan oligosaccharides.

This enzyme belongs to the family of hydrolases, specifically those glycosidases that hydrolyse O- and S-glycosyl compounds. The systematic name of this enzyme class is [(1->6)-alpha-D-xylo]-(1->4)-beta-D-glucan glucanohydrolase. Other names in common use include XEG, xyloglucan endo-beta-1,4-glucanase, xyloglucanase, xyloglucanendohydrolase, XH, and 1,4-beta-D-glucan glucanohydrolase.

[edit] Structural studies

As of late 2007, 15 structures have been solved for this class of enzymes, with PDB accession codes 2CN2, 2CN3, 2E0P, 2E4T, 2EEX, 2EJ1, 2EO7, 2EQD, 2JEM, 2JEN, 2JEP, 2JEQ, 2UWA, 2UWB, and 2UWC.

[edit] References

IUBMB entry for 3.2.1.151
BRENDA references for 3.2.1.151 (Recommended.)
PubMed references for 3.2.1.151
PubMed Central references for 3.2.1.151
Google Scholar references for 3.2.1.151
Darvill A (1999). "A xyloglucan-specific endo-beta-1,4-glucanase from Aspergillus aculeatus: expression cloning in yeast, purification and characterization of the recombinant enzyme". Glycobiology. 9: 93–100. doi:10.1093/glycob/9.1.93. PMID 9884411.
Sinitsyn AP (2004). "Specific xyloglucanases as a new class of polysaccharide-degrading enzymes". Biochim. Biophys. Acta. 1674: 268–81. PMID 15541296.