UvrABC endonuclease

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UvrABC endonuclease is a multienzyme complex in E.coli bacteria involved in DNA repair mechanism by nucleotide excision repair and it is therefore sometimes called an excinuclease. This UvrABC repair process, sometimes called the short patch process, involves the removal of 12 nucleotides where a genetic mutation has occurred followed by a DNA polymerase replacing these aberrant nucleotides with the correct nucleotides and completing the DNA repair. This enzyme complex is able to repair many different types of damage, including cyclobutyl dimer formation.

[edit] Mechanism:

1. Two UvrA proteins form a dimer and they both have ATPase/GTPase activity.
2. The UvrA dimer binds with UvrB and forms a trimer which is able to detect DNA damage.The UvrA dimer functions as the unit responsible for the detection of DNA damage, probably through a mechanism of detecting distortions in the DNA double helix.
3. The UvrB part of the trimer attaches to the double helix at the damaged site.
4. The UvrA dimer leaves and an UvrC protein comes in and binds to the UvrB monomer and hence froms a new UvrBC dimer.
5. This dimer is responsible for cleaving the nucleotides either side of the DNA damage. UvrB cleaves a phosphodiester bond 5 nucleotides downstream of the DNA damage, and the UvrC cleaves a phosphodiester bond 8 nucleotides upstream of the DNA damage and created 12 nucleotide excised segment.
6. DNA helicase II (sometimes called UvrD) then comes in and removes the excised segment by removing the base pairing. The UvrB still remain in place even though UvrC has disassociated at this stage, as UvrB may be involved in the preventing of the reannealing of the excised DNA.
7. DNA polymerase I comes in and fills in the correct nucleotides sequence and the last phosphodiester bond is completed by DNA ligase.

[edit] See also

• DNA repair
• endonuclease
• Nucleotide excision repair
• DNA

[edit] External links

• MeSH uvrABC+endonuclease

v • d • e Hydrolase, esterase: nucleases (EC 3.1.11 - 3.1.31) - includes deoxyribonuclease and ribonuclease 3.1.11-16: Exonuclease Exodeoxyribonuclease (RecBCD) Exoribonuclease (Oligonucleotidase) 3.1.21-31: Endonuclease Endodeoxyribonuclease (Deoxyribonuclease I, Deoxyribonuclease II, Deoxyribonuclease IV, Restriction enzyme, UvrABC endonuclease) Endoribonuclease (RNase III, RNase H, RNase P, RNase A, RNase T1, RNA-induced silencing complex) either deoxy- or ribo- (Aspergillus nuclease S1, Micrococcal nuclease)