Uridine monophosphate synthetase

From Wikipedia, the free encyclopedia

Uridine monophosphate synthetase (orotate phosphoribosyl transferase and orotidine-5'-decarboxylase)

PDB rendering based on 2eaw.

Available structures: 2eaw, 2jgy, 2p1f

Identifiers

Symbol(s)

UMPS; OPRT

External IDs

OMIM: 258900 MGI: 1298388 HomoloGene: 319

Gene ontology
Molecular Function:	• orotate phosphoribosyltransferase activity • orotidine-5'-phosphate decarboxylase activity • transferase activity, transferring glycosyl groups • lyase activity
Biological Process:	• 'de novo' pyrimidine base biosynthetic process • pyrimidine nucleotide biosynthetic process • UMP biosynthetic process • metabolic process • nucleoside metabolic process

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

Refseq

NM_000373 (mRNA)
NP_000364 (protein)

NM_009471 (mRNA)
NP_033497 (protein)

Location

Chr 3: 125.93 - 125.95 Mb

Chr 16: 33.87 - 33.89 Mb

Pubmed search

[1]

[2]

Uridine monophosphate synthetase (UMPS) (orotate phosphoribosyl transferase and orotidine-5'-decarboxylase) is the enzyme (EC 4.1.1.23) that catalyses the formation of uridine monophosphate (UMP), an energy carrying molecule in many important biosynthetic pathways. ^[1]

In humans, the gene that codes for this enzyme is located on the long arm of chromosome 3 (3q13).^[2] A UMP synthetase deficiency can result in a metabolic disorder called orotic aciduria.

Deficiency of this enzyme is an inherited autosomal recessive trait in Holstein cattle, and it will cause death before birth.^[3]

[edit] See also

orotidine 5'-phosphate decarboxylase

[edit] References

^ Entrez Gene: UMPS uridine monophosphate synthetase (orotate phosphoribosyl transferase and orotidine-5'-decarboxylase).
^ Qumsiyeh MB, Valentine MB, Suttle DP. (1989). Localization of the gene for uridine monophosphate synthetase to human chromosome region 3q13 by in situ hybridization. Genomics 5(1):160-2.
^ Shanks RD, Robinson JL. (1989). Embryonic mortality attributed to inherited deficiency of uridine monophosphate synthase. J Dairy Sci 72(11):3035-9.

[edit] Further reading

Suchi M, Harada N, Tsuboi T, et al. (1990). "Molecular cloning of human UMP synthase.". Adv. Exp. Med. Biol. 253A: 511-8. PMID 2624233.
Qumsiyeh MB, Valentine MB, Suttle DP (1989). "Localization of the gene for uridine monophosphate synthase to human chromosome region 3q13 by in situ hybridization.". Genomics 5 (1): 160-2. doi:10.1016/0888-7543(89)90103-1. PMID 2767686.
Suttle DP, Bugg BY, Winkler JK, Kanalas JJ (1988). "Molecular cloning and nucleotide sequence for the complete coding region of human UMP synthase.". Proc. Natl. Acad. Sci. U.S.A. 85 (6): 1754-8. doi:10.1073/pnas.85.6.1754. PMID 3279416.
Patterson D, Jones C, Morse H, et al. (1983). "Structural gene coding for multifunctional protein carrying orotate phosphoribosyltransferase and OMP decarboxylase activity is located on long arm of human chromosome 3.". Somatic Cell Genet. 9 (3): 359-74. doi:10.1007/BF01539144. PMID 6574608.
McClard RW, Black MJ, Livingstone LR, Jones ME (1981). "Isolation and initial characterization of the single polypeptide that synthesizes uridine 5'-monophosphate from orotate in Ehrlich ascites carcinoma. Purification by tandem affinity chromatography of uridine-5'-monophosphate synthase.". Biochemistry 19 (20): 4699-706. PMID 6893554.
Iannuzzi L, Di Meo GP, Ryan AM, et al. (1994). "Localization of uridine monophosphate synthase (UMPS) gene to river buffalo chromosomes by FISH.". Chromosome Res. 2 (3): 255-6. doi:10.1007/BF01553326. PMID 8069469.
Suchi M, Mizuno H, Kawai Y, et al. (1997). "Molecular cloning of the human UMP synthase gene and characterization of point mutations in two hereditary orotic aciduria families.". Am. J. Hum. Genet. 60 (3): 525-39. PMID 9042911.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899-903. doi:10.1073/pnas.242603899. PMID 12477932.
Ichikawa W, Uetake H, Shirota Y, et al. (2003). "Both gene expression for orotate phosphoribosyltransferase and its ratio to dihydropyrimidine dehydrogenase influence outcome following fluoropyrimidine-based chemotherapy for metastatic colorectal cancer.". Br. J. Cancer 89 (8): 1486-92. doi:10.1038/sj.bjc.6601335. PMID 14562021.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121-7. doi:10.1101/gr.2596504. PMID 15489334.
Miyoshi Y, Uemura H, Ishiguro H, et al. (2005). "Expression of thymidylate synthase, dihydropyrimidine dehydrogenase, thymidine phosphorylase, and orotate phosphoribosyl transferase in prostate cancer.". Prostate Cancer Prostatic Dis. 8 (3): 260-5. doi:10.1038/sj.pcan.4500817. PMID 15999119.
Ochiai T, Sugitani M, Nishimura K, et al. (2006). "Impact of orotate phosphoribosyl transferase activity as a predictor of lymph node metastasis in gastric cancer.". Oncol. Rep. 14 (4): 987-92. PMID 16142362.
Stelzl U, Worm U, Lalowski M, et al. (2005). "A human protein-protein interaction network: a resource for annotating the proteome.". Cell 122 (6): 957-68. doi:10.1016/j.cell.2005.08.029. PMID 16169070.
Kitajima M, Takita N, Hata M, et al. (2006). "The relationship between 5-fluorouracil sensitivity and single nucleotide polymorphisms of the orotate phosphoribosyl transferase gene in colorectal cancer.". Oncol. Rep. 15 (1): 161-5. PMID 16328050.
Ichikawa W, Takahashi T, Suto K, et al. (2007). "Orotate phosphoribosyltransferase gene polymorphism predicts toxicity in patients treated with bolus 5-fluorouracil regimen.". Clin. Cancer Res. 12 (13): 3928-34. doi:10.1158/1078-0432.CCR-05-2665. PMID 16818689.
Taomoto J, Yoshida K, Wada Y, et al. (2007). "Overexpression of the orotate phosphoribosyl-transferase gene enhances the effect of 5-fluorouracil on gastric cancer cell lines.". Oncology 70 (6): 458-64. doi:10.1159/000098873. PMID 17237621.
Nio Y, Toga T, Maruyama R, Fukushima M (2007). "Expression of orotate phosphoribosyl transferase in human pancreatic cancer: implication for the efficacy of uracil and tegafur-based adjuvant chemotherapy.". Oncol. Rep. 18 (1): 59-64. PMID 17549346.
Sanada Y, Yoshida K, Ohara M, Tsutani Y (2007). "Expression of orotate phosphoribosyltransferase (OPRT) in hepatobiliary and pancreatic carcinoma.". Pathol. Oncol. Res. 13 (2): 105-13. doi:PAOR.2007.13.2.0105. PMID 17607371.
Sakamoto E, Nagase H, Kobunai T, et al. (2007). "Orotate phosphoribosyltransferase expression level in tumors is a potential determinant of the efficacy of 5-fluorouracil.". Biochem. Biophys. Res. Commun. 363 (1): 216-22. doi:10.1016/j.bbrc.2007.08.164. PMID 17854773.

This EC 4.1 enzyme-related article is a stub. You can help Wikipedia by expanding it.

v • d • e Carbon-carbon lyases (EC 4.1)

4.1.1: Carboxy-lyases	Pyruvate decarboxylase -Oxaloacetate decarboxylase - Acetoacetate decarboxylase - Malonyl-CoA decarboxylase - Glutamate decarboxylase - Ornithine decarboxylase - Lysine decarboxylase - Phosphoribosylaminoimidazole carboxylase - Histidine decarboxylase - Uridine monophosphate synthetase/Orotidine 5'-phosphate decarboxylase - Aromatic L-amino acid decarboxylase - Phosphoenolpyruvate carboxylase - Pyrophosphomevalonate decarboxylase - Uroporphyrinogen III decarboxylase - RuBisCO - Phosphoenolpyruvate carboxykinase - Adenosylmethionine decarboxylase

4.1.2: Aldehyde-lyases	Aldolase A/Aldolase B - 2-hydroxyphytanoyl-CoA lyase - Threonine aldolase

4.1.3: Oxo-acid-lyases	3-hydroxy-3-methylglutaryl-CoA lyase

v • d • e

Metabolism: amino acid metabolism - nucleotide enzymes

Purine metabolism

Pyrimidine metabolism

Anabolism	CAD (Carbamoyl phosphate synthase II, Aspartate carbamoyltransferase, Dihydroorotase) Dihydroorotate dehydrogenase - Orotidine 5'-phosphate decarboxylase/Uridine monophosphate synthetase CTP synthase

Catabolism	Dihydropyrimidine dehydrogenase - Dihydropyrimidinase/DPYS - Beta-ureidopropionase/UPB1