U4atac minor spliceosomal RNA

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U4atac minor spliceosomal RNA

Type:	Gene;snRNA;splicing;
2° structure:	PMID:15987817; Daub J
Seed alignment:	Published; PMID:11911359
Avg length:	127.4 nucleotides
Avg identity:	76%

U4atac minor spliceosomal RNA is a ncRNA which is an essential component of the minor U12-type spliceosome complex. The U12-type spliceosome is required for removal of the rarer class of eukaryotic introns (AT-AC, U12-type) ^[1].

U4atac snRNA is proposed to form a base-paired complex with another spliceosomal RNA U6atac via two stem loop regions. These interacting stem loops have been shown to be required for in vivo splicing ^[2]. U4atac also contains an 3' Sm protein binding site which has been shown to be essential for splicing activity ^[2] . U4atac is the functional analog of U4 spliceosomal RNA in the major U2-type spliceosomal complex ^[2] .

The Drosophila U4atac snRNA has an additional predicted 3' stem loop terminal to the Sm binding site ^[3].

[edit] References

^ Lorkovic ZJ, Lehner R, Forstner C, Barta A; (2005). "Evolutionary conservation of minor U12-type spliceosome betweenplants and humans.". RNA 11: 1095–1107. doi:10.1261/rna.2440305. PMID 15987817.
^ ^a ^b ^c Shukla GC, Cole AJ, Dietrich RC, Padgett RA; (2002). "Domains of human U4atac snRNA required for U12-dependent splicing invivo.". Nucleic Acids Res 30: 4650–4657. doi:10.1093/nar/gkf609. PMID 12409455.
^ Padgett RA, Shukla GC; (2002). "A revised model for U4atac/U6atac snRNA base pairing.". RNA 8: 125–128. doi:10.1017/S1355838202017156. PMID 11911359.