U2 small nuclear RNA auxiliary factor 1

From Wikipedia, the free encyclopedia

PDB rendering based on 1jmt.

Available structures: 1jmt

Identifiers

Symbol(s)

U2AF1; DKFZp313J1712; FP793; RN; RNU2AF1; U2AF35; U2AFBP

External IDs

OMIM: 191317 MGI: 98884 HomoloGene: 14345

Gene ontology
Molecular Function:	• nucleotide binding • RNA binding • protein binding • zinc ion binding • metal ion binding
Cellular Component:	• nucleus • spliceosome • Cajal body
Biological Process:	• nuclear mRNA splicing, via spliceosome • RNA splicing

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

Refseq

NM_001025203 (mRNA)
NP_001020374 (protein)

NM_024187 (mRNA)
NP_077149 (protein)

Location

Chr 21: 43.39 - 43.4 Mb

Chr 17: 31.38 - 31.39 Mb

Pubmed search

[1]

[2]

U2 small nuclear RNA auxiliary factor 1, also known as U2AF1, is a human gene.^[1]

This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified.^[1]

[edit] References

^ ^a ^b Entrez Gene: U2AF1 U2 small nuclear RNA auxiliary factor 1.

[edit] Further reading

Zhang M, Zamore PD, Carmo-Fonseca M, et al. (1992). "Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit.". Proc. Natl. Acad. Sci. U.S.A. 89 (18): 8769–73. PMID 1388271.
Zamore PD, Green MR (1991). "Biochemical characterization of U2 snRNP auxiliary factor: an essential pre-mRNA splicing factor with a novel intranuclear distribution.". EMBO J. 10 (1): 207–14. PMID 1824937.
Kalcheva I, Plass C, Sait S, et al. (1994). "Comparative mapping of the imprinted U2afbpL gene on mouse chromosome 11 and human chromosome 5.". Cytogenet. Cell Genet. 68 (1-2): 19–24. PMID 7956352.
Wu JY, Maniatis T (1994). "Specific interactions between proteins implicated in splice site selection and regulated alternative splicing.". Cell 75 (6): 1061–70. PMID 8261509.
Zuo P, Maniatis T (1996). "The splicing factor U2AF35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing.". Genes Dev. 10 (11): 1356–68. PMID 8647433.
Lalioti MD, Gos A, Green MR, et al. (1997). "The gene for human U2 snRNP auxiliary factor small 35-kDa subunit (U2AF1) maps to the progressive myoclonus epilepsy (EPM1) critical region on chromosome 21q22.3.". Genomics 33 (2): 298–300. doi:10.1006/geno.1996.0196. PMID 8660980.
Hong W, Bennett M, Xiao Y, et al. (1997). "Association of U2 snRNP with the spliceosomal complex E.". Nucleic Acids Res. 25 (2): 354–61. PMID 9016565.
Tronchère H, Wang J, Fu XD (1997). "A protein related to splicing factor U2AF35 that interacts with U2AF65 and SR proteins in splicing of pre-mRNA.". Nature 388 (6640): 397–400. doi:10.1038/41137. PMID 9237760.
Wang HY, Lin W, Dyck JA, et al. (1998). "SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells.". J. Cell Biol. 140 (4): 737–50. PMID 9472028.
Rudner DZ, Kanaar R, Breger KS, Rio DC (1998). "Interaction between subunits of heterodimeric splicing factor U2AF is essential in vivo.". Mol. Cell. Biol. 18 (4): 1765–73. PMID 9528748.
Lallena MJ, Martínez C, Valcárcel J, Correas I (1998). "Functional association of nuclear protein 4.1 with pre-mRNA splicing factors.". J. Cell. Sci. 111 ( Pt 14): 1963–71. PMID 9645944.
Gozani O, Potashkin J, Reed R (1998). "A potential role for U2AF-SAP 155 interactions in recruiting U2 snRNP to the branch site.". Mol. Cell. Biol. 18 (8): 4752–60. PMID 9671485.
Neubauer G, King A, Rappsilber J, et al. (1998). "Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex.". Nat. Genet. 20 (1): 46–50. doi:10.1038/1700. PMID 9731529.
Xiao SH, Manley JL (1999). "Phosphorylation-dephosphorylation differentially affects activities of splicing factor ASF/SF2.". EMBO J. 17 (21): 6359–67. doi:10.1093/emboj/17.21.6359. PMID 9799243.
Hattori M, Fujiyama A, Taylor TD, et al. (2000). "The DNA sequence of human chromosome 21.". Nature 405 (6784): 311–9. doi:10.1038/35012518. PMID 10830953.
de Vries H, Rüegsegger U, Hübner W, et al. (2000). "Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors.". EMBO J. 19 (21): 5895–904. doi:10.1093/emboj/19.21.5895. PMID 11060040.
Gama-Carvalho M, Carvalho MP, Kehlenbach A, et al. (2001). "Nucleocytoplasmic shuttling of heterodimeric splicing factor U2AF.". J. Biol. Chem. 276 (16): 13104–12. doi:10.1074/jbc.M008759200. PMID 11118443.
Adams DJ, van der Weyden L, Mayeda A, et al. (2001). "ZNF265--a novel spliceosomal protein able to induce alternative splicing.". J. Cell Biol. 154 (1): 25–32. PMID 11448987.
Will CL, Schneider C, MacMillan AM, et al. (2001). "A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site.". EMBO J. 20 (16): 4536–46. doi:10.1093/emboj/20.16.4536. PMID 11500380.
Nesic D, Krämer A (2001). "Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation.". Mol. Cell. Biol. 21 (19): 6406–17. PMID 11533230.