U1 spliceosomal RNA

From Wikipedia, the free encyclopedia

U1 spliceosomal RNA

Type:	Gene; snRNA; splicing;
2° structure:	Published^[1]
Seed alignment:	Zwieb C, The uRNA database^[2]
Avg length:	161.1 nucleotides
Avg identity:	69%

U1 spliceosomal RNA is a small nuclear RNA (snRNA) component of the spliceosome (involved in pre-mRNA splicing). Its 5' end forms complementary base pairs with the 5' splice junction, thus defining the 5' donor site of an intron. There are significant differences in sequence and secondary structure between metazoan and yeast U1 snRNAs, the latter being much longer (568 nucleotides as compared to 164 nucleotides in humans). Nevertheless, secondary structure predictions suggest that all U1 snRNAs share a 'common core' consisting of helices I, II, the proximal region of III, and IV.^[2] This family does not contain the larger yeast sequences.

[edit] References

^ Kretzner, L; Krol A, Rosbash M (1990). "Saccharomyces cerevisiae U1 small nuclear RNA secondary structure contains both universal and yeast-specific domains". Proc Natl Acad Sci U S A 87: 851–855. doi:10.1073/pnas.87.2.851. PMID 2405391.
^ ^a ^b Zwieb, C (1997). "The uRNA database". Nucleic Acids Res 25: 102–103. doi:10.1093/nar/25.1.102. PMID 9016512.

Oubridge, C; Ito N, Evans PR, Teo CH, Nagai K (1994). "Crystal structure at 1.92 A resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin". Nature 372: 432–438. doi:10.1038/372432a0. PMID 7984237.
Katsamba, PS; Myszka DG, Laird-Offringa IA (2001). "Two functionally distinct steps mediate high affinity binding of U1A protein to U1 hairpin II RNA". J Biol Chem 276: 21476–21481. doi:10.1074/jbc.M101624200. PMID 11297556.