U12 minor spliceosomal RNA

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U12 minor spliceosomal RNA

Type:	Gene; snRNA; splicing;
2° structure:	Predicted; Griffiths-Jones S
Seed alignment:	Shukla GC and Padgett RA^[1]
Avg length:	160.4 nucleotides
Avg identity:	67%

U12 minor spliceosomal RNA is formed from U12 small nuclear (snRNA), together with U4atac/U6atac, U5, and U11 snRNAs and associated proteins, forms a spliceosome that cleaves a divergent class of low-abundance pre-mRNA introns. Although the U12 sequence is very divergent from that of U2, the two are functionally analogous.^[2] The predicted secondary structure of U12 RNA is published,^[1] but the alternative single hairpin in the 3' end shown here seems to better match the alignment of divergent Drosophila melanogaster and Arabidopsis thaliana sequences.^[3]

[edit] References

^ ^a ^b Shukla, GC; Padgett RA (1999). "Conservation of functional features of U6atac and U12 snRNAs between vertebrates and higher plants". RNA 5: 525–538. doi:10.1017/S1355838299982213. PMID 10199569.
^ Tarn, WY; Steitz JA (1997). "Pre-mRNA splicing: the discovery of a new spliceosome doubles the challenge". Trends Biochem Sci 22: 132–137. doi:10.1016/S0968-0004(97)01018-9. PMID 9149533.
^ Otake, LR; Scamborova P, Hashimoto C, Steitz JA (2002). "The divergent U12-type spliceosome is required for pre-mRNA splicing and is essential for development in Drosophila". Mol Cell 9: 439–446. doi:10.1016/S1097-2765(02)00441-0. PMID 11864616.