Two turntables and a microphone
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"Two turntables and a microphone" is the basic concept of a DJ's equipment.
This phrase describes turntables (phonographs) and a microphone connected to a mixer. The DJ uses the mixer's crossfader to fade between two songs playing on the turntables. Fading often includes beatmatching. Live hip hop music also often has an MC rapping into the microphone. In nightclubs the microphone is usually used only for announcements.
The set up was first used by Jimmy Savile in 1946. This phrase is used in the chorus of the song "Needle to the Groove" by Mantronix and "Where It's At" on Beck's album Odelay.
This phrase is also referenced in season 5 of the hit Canadian TV show Trailer Park Boys when Ricky steals J-Roc's DJ equipment. It is unclear whether or not this is an intentional reference to any of the songs in which this line appears.