TOP2B

From Wikipedia, the free encyclopedia

Topoisomerase (DNA) II beta 180kDa

Identifiers

TOP2B; TOPIIB; top2beta

External IDs

OMIM: 126431 MGI: 98791 HomoloGene: 68155

Gene ontology
Molecular Function:	• nucleotide binding • chromatin binding • DNA topoisomerase (ATP-hydrolyzing) activity • protein kinase C binding • ATP binding • protein C-terminus binding • histone deacetylase binding • protein heterodimerization activity
Cellular Component:	• heterochromatin • nucleus • nucleoplasm • chromosome • nucleolus • cytosol
Biological Process:	• neuron migration • DNA topological change • axonogenesis • forebrain development

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

Refseq

NM_001068 (mRNA)
NP_001059 (protein)

NM_009409 (mRNA)
NP_033435 (protein)

Location

Chr 3: 25.61 - 25.68 Mb

Chr 14: 15.16 - 15.22 Mb

Pubmed search

[1]

[2]

Topoisomerase (DNA) II beta 180kDa, also known as TOP2B, is a human gene.

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results in two transcript variants; however, the second variant has not yet been fully described.^[1]

[edit] References

^ Entrez Gene: TOP2B topoisomerase (DNA) II beta 180kDa.

[edit] Further reading

Champoux JJ (2002). "DNA topoisomerases: structure, function, and mechanism.". Annu. Rev. Biochem. 70: 369–413. doi:10.1146/annurev.biochem.70.1.369. PMID 11395412.
Tan KB, Dorman TE, Falls KM, et al. (1992). "Topoisomerase II alpha and topoisomerase II beta genes: characterization and mapping to human chromosomes 17 and 3, respectively.". Cancer Res. 52 (1): 231–4. PMID 1309226.
Jenkins JR, Ayton P, Jones T, et al. (1993). "Isolation of cDNA clones encoding the beta isozyme of human DNA topoisomerase II and localisation of the gene to chromosome 3p24.". Nucleic Acids Res. 20 (21): 5587–92. PMID 1333583.
Austin CA, Fisher LM (1990). "Isolation and characterization of a human cDNA clone encoding a novel DNA topoisomerase II homologue from HeLa cells.". FEBS Lett. 266 (1-2): 115–7. PMID 2163884.
Chung TD, Drake FH, Tan KB, et al. (1990). "Characterization and immunological identification of cDNA clones encoding two human DNA topoisomerase II isozymes.". Proc. Natl. Acad. Sci. U.S.A. 86 (23): 9431–5. PMID 2556712.
Austin CA, Sng JH, Patel S, Fisher LM (1993). "Novel HeLa topoisomerase II is the II beta isoform: complete coding sequence and homology with other type II topoisomerases.". Biochim. Biophys. Acta 1172 (3): 283–91. PMID 8383537.
Davies SL, Jenkins JR, Hickson ID (1993). "Human cells express two differentially spliced forms of topoisomerase II beta mRNA.". Nucleic Acids Res. 21 (16): 3719–23. PMID 8396237.
Nakano H, Yamazaki T, Miyatake S, et al. (1996). "Specific interaction of topoisomerase II beta and the CD3 epsilon chain of the T cell receptor complex.". J. Biol. Chem. 271 (11): 6483–9. PMID 8626450.
Biersack H, Jensen S, Gromova I, et al. (1996). "Active heterodimers are formed from human DNA topoisomerase II alpha and II beta isoforms.". Proc. Natl. Acad. Sci. U.S.A. 93 (16): 8288–93. PMID 8710863.
Meyer KN, Kjeldsen E, Straub T, et al. (1997). "Cell cycle-coupled relocation of types I and II topoisomerases and modulation of catalytic enzyme activities.". J. Cell Biol. 136 (4): 775–88. PMID 9049244.
Fukunaga R, Hunter T (1997). "MNK1, a new MAP kinase-activated protein kinase, isolated by a novel expression screening method for identifying protein kinase substrates.". EMBO J. 16 (8): 1921–33. doi:10.1093/emboj/16.8.1921. PMID 9155018.
Ng SW, Liu Y, Schnipper LE (1998). "Cloning and characterization of the 5'-flanking sequence for the human DNA topoisomerase II beta gene.". Gene 203 (2): 113–9. PMID 9426241.
Yamane K, Kawabata M, Tsuruo T (1998). "A DNA-topoisomerase-II-binding protein with eight repeating regions similar to DNA-repair enzymes and to a cell-cycle regulator.". Eur. J. Biochem. 250 (3): 794–9. PMID 9461304.
Cowell IG, Willmore E, Chalton D, et al. (1998). "Nuclear distribution of human DNA topoisomerase IIbeta: a nuclear targeting signal resides in the 116-residue C-terminal tail.". Exp. Cell Res. 243 (2): 232–40. doi:10.1006/excr.1998.4150. PMID 9743583.
Lang AJ, Mirski SE, Cummings HJ, et al. (1998). "Structural organization of the human TOP2A and TOP2B genes.". Gene 221 (2): 255–66. PMID 9795238.
Sng JH, Heaton VJ, Bell M, et al. (1999). "Molecular cloning and characterization of the human topoisomerase IIalpha and IIbeta genes: evidence for isoform evolution through gene duplication.". Biochim. Biophys. Acta 1444 (3): 395–406. PMID 10095062.
Bjergbaek L, Jensen S, Westergaard O, Andersen AH (1999). "Using a biochemical approach to identify the primary dimerization regions in human DNA topoisomerase IIalpha.". J. Biol. Chem. 274 (37): 26529–36. PMID 10473615.
Cowell IG, Okorokov AL, Cutts SA, et al. (2000). "Human topoisomerase IIalpha and IIbeta interact with the C-terminal region of p53.". Exp. Cell Res. 255 (1): 86–94. doi:10.1006/excr.1999.4772. PMID 10666337.
Mao Y, Desai SD, Liu LF (2000). "SUMO-1 conjugation to human DNA topoisomerase II isozymes.". J. Biol. Chem. 275 (34): 26066–73. doi:10.1074/jbc.M001831200. PMID 10862613.
Mirski SE, Voskoglou-Nomikos T, Young LC, et al. (2000). "Simultaneous quantitation of topoisomerase II alpha and beta isoform mRNAs in lung tumor cells and normal and malignant lung tissue.". Lab. Invest. 80 (6): 787–95. PMID 10879730.