Toluene dioxygenase

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In enzymology, a toluene dioxygenase (EC 1.14.12.11) is an enzyme that catalyzes the chemical reaction

toluene + NADH + H⁺ + O₂ $\rightleftharpoons$ (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD⁺

The 4 substrates of this enzyme are toluene, NADH, H⁺, and O₂, whereas its two products are (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol and NAD⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of two atoms o oxygen into the other donor. The systematic name of this enzyme class is toluene,NADH:oxygen oxidoreductase (1,2-hydroxylating). This enzyme is also called toluene 2,3-dioxygenase. This enzyme participates in toluene and xylene degradation.

[edit] References

IUBMB entry for 1.14.12.11
BRENDA references for 1.14.12.11 (Recommended.)
PubMed references for 1.14.12.11
PubMed Central references for 1.14.12.11
Google Scholar references for 1.14.12.11
Renganathan V (1989). "Possible involvement of toluene-2,3-dioxygenase in defluorination of 3-fluoro-substituted benzenes by toluene-degrading Pseudomonas sp strain T-12". Appl. Exp. Microbiol. 55: 330–334.
Subramanian V, Liu TN, Yeh WK, Gibson DT (1979). "Toluene dioxygenase: purification of an iron-sulfur protein by affinity chromatography". Biochem. Biophys. Res. Commun. 91: 1131–9. PMID 526270.