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Thermus thermophilus is a gram negative eubacterium used in a range of biotechnological applications, including as a model organism for genetic manipulation and systems biology. The bacterium is extremely thermophilic, with an optimal growth temperature of about 65ºC. Thermus thermophilus was originally isolated from a thermal vent environment in Japan. T. thermophilus is classified into several strains, in which HB8 and HB27 are most common and their genome analyses were independently completed in 2004.[1]
[edit] Biotechnological applications of Thermus thermophilus enzymes
- rTth DNA polymerase is a recombinant thermostable DNA polymerase derived from Thermus thermophilus, with optimal activity at 70-80ºC, used in some PCR applications. The enzyme possesses efficient reverse transcriptase activity in the presence of manganese.
[edit] External links
[edit] References
- ^ Henne A et al. (May 2004). "The genome sequence of the extreme thermophile Thermus thermophilus". Nat Biotechnol. 22 (5): 547–53. doi:10.1038/nbt956. PMID 15064768.
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