User talk:Terri G

From Wikipedia, the free encyclopedia

Added some background Terri G 14:22, 10 April 2006 (UTC)

The current History of Science Collaboration of the Month is Louis Pasteur.

--ragesoss 15:07, 2 June 2006 (UTC)

Contents 1 Dixie Mission advice 2 FPC requires addtional input 3 Thanks! 4 Oops, copied wrong file - P. aeruginosa

[edit] Dixie Mission advice

Hi Terri, I just wanted to drop a note to thank you for the suggestion on stubifying the red links. For some reason, it never crossed my mind that a stub was better than a red link. I fixed them as you suggested, and again, thank you for the advice. ~ (The Rebel At) ~ 13:02, 11 October 2006 (UTC)

[edit] FPC requires addtional input

You voted on Wikipedia:Featured picture candidates/Vernier caliper, but a new version has been proposed and we need to close the vote. If you could clarify your vote that would be very helpful. ed g2s • talk 15:01, 27 November 2006 (UTC)

[edit] Thanks!

	Thanks again for your comments in Peer Review - White Deer Hole Creek made featured article! Take care, Ruhrfisch 17:11, 11 December 2006 (UTC)

[edit] Oops, copied wrong file - P. aeruginosa

Hello Terry. Please accept all my apologies indeed for the inadvertent cut-and-paste last year in P. aeruginosa article. Just came back a few days ago, found post about it on my talk page and it's been on my to-do list since then. I use at least two files when I edit, usually more, and had copied an unfinished version back to the article page :-( It was my last edit on it too (end of session, tired, closed several articles at once) and I didn't go back to that article after that or I could not have missed the mistake, it was clear with that “our work” left in it. The next day I did have a feeling something like that had happened somewhere, but couldn't think of where, had had too many articles on the go at that time.

From what you wrote last year, you are among the et al. who have worked on these experiments. Since I'm here for my atonement let me tell you how very impressed I have been at reading about these experiments and their results. Hem, it's even embarrassing, come to think of it. This article, pointing out at the vital importance of biofilm, has opened a whole fascinating level of reflections to me. Call that multi-dimensional because it's even made me completely rethink the whole process of tooth-brushing, take that for daily reminder; see another dimension to the concept of network; and no end in sight to the situations to which that concept of biofilm can be applied. I was awed, what can I say? This article could not have had a more capital impact on me than the apple's had to Newton. Now that you know about this you cannot but forgive me for any offence from that unvoluntary slip last year in reporting that work as I beg you to please do.

Of course I find this biofilm concept no less interesting now as then (although admitedly the state of exaltation has abated somewhat). Since the slip brought me back to the P. aeruginosa article, I hope I am not creating further offence by saying I still think that the details of your work are not out of place in that paragraph, and that its reading supports well the importance of the concept of biofilms. So I have gone back on what I had left on the article page last year, and propose this to you with hope that you will consider it as an acceptable presentation of your work:

Walker et al. reported in 2001, that when P. aeruginosa colonizes roots it protects itself by creating a biofilm which resists antibiotics secretions from the roots and spreads around these before the plant dies. Arabidopsis and sweet basil (Ocimum basilicum) died seven days after inoculation with pathogenic strains P. aeruginosa PAO1 and PA14.This infection brings the secretion of rosmarinic acid in sweet basil roots. Rosmarinic acid is a caffeic acid ester; among other fonctions, it demonstrates antibacterial activity when the root is inoculated with planktonic cells of both P. aeruginosa strains at a minimum inhibitory concentration of 3 µg mL-1 in vitro. P. aeruginosa resisted the microbicidal effects of rosmarinic acid by forming a biofilm before sweet basil's root exudates secreted minimum inhibitory concentration levels of rosmarinic acid. The plant subsequently died.

Sweet basil roots and Arabidopsis root exudates, were supplied with rosmarinic acid to induce their own secreting before being infected with P. aeruginosa. Large clusters of dead P. aeruginosa were then seen with a confocal scanning laser microscopy, on the root surface of Arabidopsis and sweet basil; there was no formation of biofilm; and the plant did not die. Three quorum-sensing mutants PAO210 (rhlI), PAO214 (lasI), and PAO216 (lasI rhlI) were pathogenic to Arabidopsis. Root exudates of Arabidopsis do not naturally secrete rosmarinic acid. But of the three, only PAO214 was pathogenic to sweet basil, and its biofilm was similar to biofilms formed by wild-type strains of P. aeruginosa.(ref Walker)

I am grateful for any comment you would care to make. If you do so, as I am not quite sure of how wiki works for alerts for messages can I please ask you to let me know you've done so and where, by a note on my 'talk' page here User_talk:Basicdesign. Thank you very much. Françoise Basicdesign (talk) 16:54, 7 December 2007 (UTC)